Cargando…

Occlusive dressing-induced secretomes influence the migration and proliferation of mesenchymal stem cells and fibroblasts differently

BACKGROUND: Fingertip injuries treated with occlusive dressings (ODs) lead to nearly scar-free, functionally, and aesthetically pleasing results. We hypothesized that paracrine factors in the wound fluid (secretome) may influence migration and proliferation of mesenchymal stem cells (MSCs) and fibro...

Descripción completa

Detalles Bibliográficos
Autores principales: Cerny, Michael K., Hopfner, Ursula, Kirsch, Manuela, Haas, Elisabeth-Maria, Wu, Fan, Giunta, Riccardo, Machens, Hans-Guenther, Duscher, Dominik, Erne, Holger, Schilling, Arndt F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6306003/
https://www.ncbi.nlm.nih.gov/pubmed/30585140
http://dx.doi.org/10.1186/s40001-018-0357-2
_version_ 1783382692922916864
author Cerny, Michael K.
Hopfner, Ursula
Kirsch, Manuela
Haas, Elisabeth-Maria
Wu, Fan
Giunta, Riccardo
Machens, Hans-Guenther
Duscher, Dominik
Erne, Holger
Schilling, Arndt F.
author_facet Cerny, Michael K.
Hopfner, Ursula
Kirsch, Manuela
Haas, Elisabeth-Maria
Wu, Fan
Giunta, Riccardo
Machens, Hans-Guenther
Duscher, Dominik
Erne, Holger
Schilling, Arndt F.
author_sort Cerny, Michael K.
collection PubMed
description BACKGROUND: Fingertip injuries treated with occlusive dressings (ODs) lead to nearly scar-free, functionally, and aesthetically pleasing results. We hypothesized that paracrine factors in the wound fluid (secretome) may influence migration and proliferation of mesenchymal stem cells (MSCs) and fibroblasts and modulate the wound-healing process. METHODS: We could collect wound fluid samples from 4 fingertip injuries and 7 split skin donor sites at the 5th day during dressing change. Blood serum samples served as controls. The proliferation rate of MSCs and fibroblasts (HS27) was continuously measured through impedance analysis for 60 h and by Alamarblue analysis after 72 h. Cell migration was evaluated continuously for 15 h and confirmed by the in vitro wound-healing assay. RESULTS: Migration of MSCs under the influence of both wound fluids was significantly faster than controls from 4 to 6 h after incubation and reversed after 9 h. MSC proliferation in wound fluid groups showed a significant increase at 5 and 10 h and was significantly decreased after 45 h. Fibroblasts in wound fluid groups showed overall a significant increase in migration and a significant decrease in proliferation compared to controls. CONCLUSION: OD-induced secretomes influence MSCs and fibroblasts and thereby possibly modulate wound healing and scar tissue formation.
format Online
Article
Text
id pubmed-6306003
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-63060032019-01-02 Occlusive dressing-induced secretomes influence the migration and proliferation of mesenchymal stem cells and fibroblasts differently Cerny, Michael K. Hopfner, Ursula Kirsch, Manuela Haas, Elisabeth-Maria Wu, Fan Giunta, Riccardo Machens, Hans-Guenther Duscher, Dominik Erne, Holger Schilling, Arndt F. Eur J Med Res Research BACKGROUND: Fingertip injuries treated with occlusive dressings (ODs) lead to nearly scar-free, functionally, and aesthetically pleasing results. We hypothesized that paracrine factors in the wound fluid (secretome) may influence migration and proliferation of mesenchymal stem cells (MSCs) and fibroblasts and modulate the wound-healing process. METHODS: We could collect wound fluid samples from 4 fingertip injuries and 7 split skin donor sites at the 5th day during dressing change. Blood serum samples served as controls. The proliferation rate of MSCs and fibroblasts (HS27) was continuously measured through impedance analysis for 60 h and by Alamarblue analysis after 72 h. Cell migration was evaluated continuously for 15 h and confirmed by the in vitro wound-healing assay. RESULTS: Migration of MSCs under the influence of both wound fluids was significantly faster than controls from 4 to 6 h after incubation and reversed after 9 h. MSC proliferation in wound fluid groups showed a significant increase at 5 and 10 h and was significantly decreased after 45 h. Fibroblasts in wound fluid groups showed overall a significant increase in migration and a significant decrease in proliferation compared to controls. CONCLUSION: OD-induced secretomes influence MSCs and fibroblasts and thereby possibly modulate wound healing and scar tissue formation. BioMed Central 2018-12-26 /pmc/articles/PMC6306003/ /pubmed/30585140 http://dx.doi.org/10.1186/s40001-018-0357-2 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Cerny, Michael K.
Hopfner, Ursula
Kirsch, Manuela
Haas, Elisabeth-Maria
Wu, Fan
Giunta, Riccardo
Machens, Hans-Guenther
Duscher, Dominik
Erne, Holger
Schilling, Arndt F.
Occlusive dressing-induced secretomes influence the migration and proliferation of mesenchymal stem cells and fibroblasts differently
title Occlusive dressing-induced secretomes influence the migration and proliferation of mesenchymal stem cells and fibroblasts differently
title_full Occlusive dressing-induced secretomes influence the migration and proliferation of mesenchymal stem cells and fibroblasts differently
title_fullStr Occlusive dressing-induced secretomes influence the migration and proliferation of mesenchymal stem cells and fibroblasts differently
title_full_unstemmed Occlusive dressing-induced secretomes influence the migration and proliferation of mesenchymal stem cells and fibroblasts differently
title_short Occlusive dressing-induced secretomes influence the migration and proliferation of mesenchymal stem cells and fibroblasts differently
title_sort occlusive dressing-induced secretomes influence the migration and proliferation of mesenchymal stem cells and fibroblasts differently
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6306003/
https://www.ncbi.nlm.nih.gov/pubmed/30585140
http://dx.doi.org/10.1186/s40001-018-0357-2
work_keys_str_mv AT cernymichaelk occlusivedressinginducedsecretomesinfluencethemigrationandproliferationofmesenchymalstemcellsandfibroblastsdifferently
AT hopfnerursula occlusivedressinginducedsecretomesinfluencethemigrationandproliferationofmesenchymalstemcellsandfibroblastsdifferently
AT kirschmanuela occlusivedressinginducedsecretomesinfluencethemigrationandproliferationofmesenchymalstemcellsandfibroblastsdifferently
AT haaselisabethmaria occlusivedressinginducedsecretomesinfluencethemigrationandproliferationofmesenchymalstemcellsandfibroblastsdifferently
AT wufan occlusivedressinginducedsecretomesinfluencethemigrationandproliferationofmesenchymalstemcellsandfibroblastsdifferently
AT giuntariccardo occlusivedressinginducedsecretomesinfluencethemigrationandproliferationofmesenchymalstemcellsandfibroblastsdifferently
AT machenshansguenther occlusivedressinginducedsecretomesinfluencethemigrationandproliferationofmesenchymalstemcellsandfibroblastsdifferently
AT duscherdominik occlusivedressinginducedsecretomesinfluencethemigrationandproliferationofmesenchymalstemcellsandfibroblastsdifferently
AT erneholger occlusivedressinginducedsecretomesinfluencethemigrationandproliferationofmesenchymalstemcellsandfibroblastsdifferently
AT schillingarndtf occlusivedressinginducedsecretomesinfluencethemigrationandproliferationofmesenchymalstemcellsandfibroblastsdifferently