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Occlusive dressing-induced secretomes influence the migration and proliferation of mesenchymal stem cells and fibroblasts differently
BACKGROUND: Fingertip injuries treated with occlusive dressings (ODs) lead to nearly scar-free, functionally, and aesthetically pleasing results. We hypothesized that paracrine factors in the wound fluid (secretome) may influence migration and proliferation of mesenchymal stem cells (MSCs) and fibro...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6306003/ https://www.ncbi.nlm.nih.gov/pubmed/30585140 http://dx.doi.org/10.1186/s40001-018-0357-2 |
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author | Cerny, Michael K. Hopfner, Ursula Kirsch, Manuela Haas, Elisabeth-Maria Wu, Fan Giunta, Riccardo Machens, Hans-Guenther Duscher, Dominik Erne, Holger Schilling, Arndt F. |
author_facet | Cerny, Michael K. Hopfner, Ursula Kirsch, Manuela Haas, Elisabeth-Maria Wu, Fan Giunta, Riccardo Machens, Hans-Guenther Duscher, Dominik Erne, Holger Schilling, Arndt F. |
author_sort | Cerny, Michael K. |
collection | PubMed |
description | BACKGROUND: Fingertip injuries treated with occlusive dressings (ODs) lead to nearly scar-free, functionally, and aesthetically pleasing results. We hypothesized that paracrine factors in the wound fluid (secretome) may influence migration and proliferation of mesenchymal stem cells (MSCs) and fibroblasts and modulate the wound-healing process. METHODS: We could collect wound fluid samples from 4 fingertip injuries and 7 split skin donor sites at the 5th day during dressing change. Blood serum samples served as controls. The proliferation rate of MSCs and fibroblasts (HS27) was continuously measured through impedance analysis for 60 h and by Alamarblue analysis after 72 h. Cell migration was evaluated continuously for 15 h and confirmed by the in vitro wound-healing assay. RESULTS: Migration of MSCs under the influence of both wound fluids was significantly faster than controls from 4 to 6 h after incubation and reversed after 9 h. MSC proliferation in wound fluid groups showed a significant increase at 5 and 10 h and was significantly decreased after 45 h. Fibroblasts in wound fluid groups showed overall a significant increase in migration and a significant decrease in proliferation compared to controls. CONCLUSION: OD-induced secretomes influence MSCs and fibroblasts and thereby possibly modulate wound healing and scar tissue formation. |
format | Online Article Text |
id | pubmed-6306003 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-63060032019-01-02 Occlusive dressing-induced secretomes influence the migration and proliferation of mesenchymal stem cells and fibroblasts differently Cerny, Michael K. Hopfner, Ursula Kirsch, Manuela Haas, Elisabeth-Maria Wu, Fan Giunta, Riccardo Machens, Hans-Guenther Duscher, Dominik Erne, Holger Schilling, Arndt F. Eur J Med Res Research BACKGROUND: Fingertip injuries treated with occlusive dressings (ODs) lead to nearly scar-free, functionally, and aesthetically pleasing results. We hypothesized that paracrine factors in the wound fluid (secretome) may influence migration and proliferation of mesenchymal stem cells (MSCs) and fibroblasts and modulate the wound-healing process. METHODS: We could collect wound fluid samples from 4 fingertip injuries and 7 split skin donor sites at the 5th day during dressing change. Blood serum samples served as controls. The proliferation rate of MSCs and fibroblasts (HS27) was continuously measured through impedance analysis for 60 h and by Alamarblue analysis after 72 h. Cell migration was evaluated continuously for 15 h and confirmed by the in vitro wound-healing assay. RESULTS: Migration of MSCs under the influence of both wound fluids was significantly faster than controls from 4 to 6 h after incubation and reversed after 9 h. MSC proliferation in wound fluid groups showed a significant increase at 5 and 10 h and was significantly decreased after 45 h. Fibroblasts in wound fluid groups showed overall a significant increase in migration and a significant decrease in proliferation compared to controls. CONCLUSION: OD-induced secretomes influence MSCs and fibroblasts and thereby possibly modulate wound healing and scar tissue formation. BioMed Central 2018-12-26 /pmc/articles/PMC6306003/ /pubmed/30585140 http://dx.doi.org/10.1186/s40001-018-0357-2 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Cerny, Michael K. Hopfner, Ursula Kirsch, Manuela Haas, Elisabeth-Maria Wu, Fan Giunta, Riccardo Machens, Hans-Guenther Duscher, Dominik Erne, Holger Schilling, Arndt F. Occlusive dressing-induced secretomes influence the migration and proliferation of mesenchymal stem cells and fibroblasts differently |
title | Occlusive dressing-induced secretomes influence the migration and proliferation of mesenchymal stem cells and fibroblasts differently |
title_full | Occlusive dressing-induced secretomes influence the migration and proliferation of mesenchymal stem cells and fibroblasts differently |
title_fullStr | Occlusive dressing-induced secretomes influence the migration and proliferation of mesenchymal stem cells and fibroblasts differently |
title_full_unstemmed | Occlusive dressing-induced secretomes influence the migration and proliferation of mesenchymal stem cells and fibroblasts differently |
title_short | Occlusive dressing-induced secretomes influence the migration and proliferation of mesenchymal stem cells and fibroblasts differently |
title_sort | occlusive dressing-induced secretomes influence the migration and proliferation of mesenchymal stem cells and fibroblasts differently |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6306003/ https://www.ncbi.nlm.nih.gov/pubmed/30585140 http://dx.doi.org/10.1186/s40001-018-0357-2 |
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