Evaluation of two enzyme-linked immunosorbent assays for the detection of antibodies against equine arteritis virus

In order to establish an efficient system for serological diagnosis of equine viral arteritis in Japan, we compared enzyme-linked immunosorbent assays (ELISAs) provided by two manufacturers (Nisseiken Co., Ltd., Tokyo, Japan, and VMRD Inc., Pullman, WA, U.S.A.) by testing a series of horse sera. The...

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Autores principales: BANNAI, Hiroshi, NEMOTO, Manabu, TSUJIMURA, Koji, YAMANAKA, Takashi, KOKADO, Hiroshi, KONDO, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Equine Science 2018
Materias:
Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6306293/
https://www.ncbi.nlm.nih.gov/pubmed/30607135
http://dx.doi.org/10.1294/jes.29.111
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author BANNAI, Hiroshi
NEMOTO, Manabu
TSUJIMURA, Koji
YAMANAKA, Takashi
KOKADO, Hiroshi
KONDO, Takashi
author_facet BANNAI, Hiroshi
NEMOTO, Manabu
TSUJIMURA, Koji
YAMANAKA, Takashi
KOKADO, Hiroshi
KONDO, Takashi
author_sort BANNAI, Hiroshi
collection PubMed
description In order to establish an efficient system for serological diagnosis of equine viral arteritis in Japan, we compared enzyme-linked immunosorbent assays (ELISAs) provided by two manufacturers (Nisseiken Co., Ltd., Tokyo, Japan, and VMRD Inc., Pullman, WA, U.S.A.) by testing a series of horse sera. The results revealed that 159 of 160 virus-neutralizing (VN) antibody-positive serum samples were positive in both the Nisseiken-ELISA and VMRD-ELISA. Of the VN-negative sera (n=157), 134 and 154 samples were negative in the Nisseiken-ELISA and VMRD-ELISA, respectively. Sensitivity was 99.4% for both the Nisseiken-ELISA and VMRD-ELISA. The specificity of the VMRD-ELISA (98.1%) was significantly higher than that of the Nisseiken-ELISA (85.4%, P<0.05). The diagnostic performance of the VMRD-ELISA was superior to that of the Nisseiken-ELISA because of this greater specificity.
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spelling pubmed-63062932019-01-03 Evaluation of two enzyme-linked immunosorbent assays for the detection of antibodies against equine arteritis virus BANNAI, Hiroshi NEMOTO, Manabu TSUJIMURA, Koji YAMANAKA, Takashi KOKADO, Hiroshi KONDO, Takashi J Equine Sci Note In order to establish an efficient system for serological diagnosis of equine viral arteritis in Japan, we compared enzyme-linked immunosorbent assays (ELISAs) provided by two manufacturers (Nisseiken Co., Ltd., Tokyo, Japan, and VMRD Inc., Pullman, WA, U.S.A.) by testing a series of horse sera. The results revealed that 159 of 160 virus-neutralizing (VN) antibody-positive serum samples were positive in both the Nisseiken-ELISA and VMRD-ELISA. Of the VN-negative sera (n=157), 134 and 154 samples were negative in the Nisseiken-ELISA and VMRD-ELISA, respectively. Sensitivity was 99.4% for both the Nisseiken-ELISA and VMRD-ELISA. The specificity of the VMRD-ELISA (98.1%) was significantly higher than that of the Nisseiken-ELISA (85.4%, P<0.05). The diagnostic performance of the VMRD-ELISA was superior to that of the Nisseiken-ELISA because of this greater specificity. The Japanese Society of Equine Science 2018-12-18 2018-12 /pmc/articles/PMC6306293/ /pubmed/30607135 http://dx.doi.org/10.1294/jes.29.111 Text en ©2018 The Japanese Society of Equine Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Note
BANNAI, Hiroshi
NEMOTO, Manabu
TSUJIMURA, Koji
YAMANAKA, Takashi
KOKADO, Hiroshi
KONDO, Takashi
Evaluation of two enzyme-linked immunosorbent assays for the detection of antibodies against equine arteritis virus
title Evaluation of two enzyme-linked immunosorbent assays for the detection of antibodies against equine arteritis virus
title_full Evaluation of two enzyme-linked immunosorbent assays for the detection of antibodies against equine arteritis virus
title_fullStr Evaluation of two enzyme-linked immunosorbent assays for the detection of antibodies against equine arteritis virus
title_full_unstemmed Evaluation of two enzyme-linked immunosorbent assays for the detection of antibodies against equine arteritis virus
title_short Evaluation of two enzyme-linked immunosorbent assays for the detection of antibodies against equine arteritis virus
title_sort evaluation of two enzyme-linked immunosorbent assays for the detection of antibodies against equine arteritis virus
topic Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6306293/
https://www.ncbi.nlm.nih.gov/pubmed/30607135
http://dx.doi.org/10.1294/jes.29.111
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