Cargando…

Development and Evaluation of an Injectable Chitosan/β-Glycerophosphate Paste as a Local Antibiotic Delivery System for Trauma Care

Complex open musculoskeletal wounds are a leading cause of morbidity worldwide, partially due to a high risk of bacterial contamination. Local delivery systems may be used as adjunctive therapies to prevent infection, but they may be nondegradable, possess inadequate wound coverage, or migrate from...

Descripción completa

Detalles Bibliográficos
Autores principales: Boles, Logan, Alexander, Christopher, Pace, Leslie, Haggard, Warren, Bumgardner, Joel, Jennings, Jessica
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6306749/
https://www.ncbi.nlm.nih.gov/pubmed/30322006
http://dx.doi.org/10.3390/jfb9040056
_version_ 1783382848319782912
author Boles, Logan
Alexander, Christopher
Pace, Leslie
Haggard, Warren
Bumgardner, Joel
Jennings, Jessica
author_facet Boles, Logan
Alexander, Christopher
Pace, Leslie
Haggard, Warren
Bumgardner, Joel
Jennings, Jessica
author_sort Boles, Logan
collection PubMed
description Complex open musculoskeletal wounds are a leading cause of morbidity worldwide, partially due to a high risk of bacterial contamination. Local delivery systems may be used as adjunctive therapies to prevent infection, but they may be nondegradable, possess inadequate wound coverage, or migrate from the wound site. To address this issue, a thermo-responsive, injectable chitosan paste was fabricated by incorporating beta-glycerophosphate. The efficacy of thermo-paste as an adjunctive infection prevention tool was evaluated in terms of cytocompatibility, degradation, antibacterial, injectability, and inflammation properties. In vitro studies demonstrated thermo-paste may be loaded with amikacin and vancomycin and release inhibitory levels for at least 3 days. Further, approximately 60% of thermo-paste was enzymatically degraded within 7 days in vitro. The viability of cells exposed to thermo-paste exceeded ISO 10993-5 standards with approximately 73% relative viability of a control chitosan sponge. The ejection force of thermo-paste, approximately 20 N, was lower than previously studied paste formulations and within relevant clinical ejection force ranges. An in vivo murine biocompatibility study demonstrated that thermo-paste induced minimal inflammation after implantation for 7 days, similar to previously developed chitosan pastes. Results from these preliminary preclinical studies indicate that thermo-paste shows promise for further development as an antibiotic delivery system for infection prevention.
format Online
Article
Text
id pubmed-6306749
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-63067492019-01-02 Development and Evaluation of an Injectable Chitosan/β-Glycerophosphate Paste as a Local Antibiotic Delivery System for Trauma Care Boles, Logan Alexander, Christopher Pace, Leslie Haggard, Warren Bumgardner, Joel Jennings, Jessica J Funct Biomater Article Complex open musculoskeletal wounds are a leading cause of morbidity worldwide, partially due to a high risk of bacterial contamination. Local delivery systems may be used as adjunctive therapies to prevent infection, but they may be nondegradable, possess inadequate wound coverage, or migrate from the wound site. To address this issue, a thermo-responsive, injectable chitosan paste was fabricated by incorporating beta-glycerophosphate. The efficacy of thermo-paste as an adjunctive infection prevention tool was evaluated in terms of cytocompatibility, degradation, antibacterial, injectability, and inflammation properties. In vitro studies demonstrated thermo-paste may be loaded with amikacin and vancomycin and release inhibitory levels for at least 3 days. Further, approximately 60% of thermo-paste was enzymatically degraded within 7 days in vitro. The viability of cells exposed to thermo-paste exceeded ISO 10993-5 standards with approximately 73% relative viability of a control chitosan sponge. The ejection force of thermo-paste, approximately 20 N, was lower than previously studied paste formulations and within relevant clinical ejection force ranges. An in vivo murine biocompatibility study demonstrated that thermo-paste induced minimal inflammation after implantation for 7 days, similar to previously developed chitosan pastes. Results from these preliminary preclinical studies indicate that thermo-paste shows promise for further development as an antibiotic delivery system for infection prevention. MDPI 2018-10-12 /pmc/articles/PMC6306749/ /pubmed/30322006 http://dx.doi.org/10.3390/jfb9040056 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Boles, Logan
Alexander, Christopher
Pace, Leslie
Haggard, Warren
Bumgardner, Joel
Jennings, Jessica
Development and Evaluation of an Injectable Chitosan/β-Glycerophosphate Paste as a Local Antibiotic Delivery System for Trauma Care
title Development and Evaluation of an Injectable Chitosan/β-Glycerophosphate Paste as a Local Antibiotic Delivery System for Trauma Care
title_full Development and Evaluation of an Injectable Chitosan/β-Glycerophosphate Paste as a Local Antibiotic Delivery System for Trauma Care
title_fullStr Development and Evaluation of an Injectable Chitosan/β-Glycerophosphate Paste as a Local Antibiotic Delivery System for Trauma Care
title_full_unstemmed Development and Evaluation of an Injectable Chitosan/β-Glycerophosphate Paste as a Local Antibiotic Delivery System for Trauma Care
title_short Development and Evaluation of an Injectable Chitosan/β-Glycerophosphate Paste as a Local Antibiotic Delivery System for Trauma Care
title_sort development and evaluation of an injectable chitosan/β-glycerophosphate paste as a local antibiotic delivery system for trauma care
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6306749/
https://www.ncbi.nlm.nih.gov/pubmed/30322006
http://dx.doi.org/10.3390/jfb9040056
work_keys_str_mv AT boleslogan developmentandevaluationofaninjectablechitosanbglycerophosphatepasteasalocalantibioticdeliverysystemfortraumacare
AT alexanderchristopher developmentandevaluationofaninjectablechitosanbglycerophosphatepasteasalocalantibioticdeliverysystemfortraumacare
AT paceleslie developmentandevaluationofaninjectablechitosanbglycerophosphatepasteasalocalantibioticdeliverysystemfortraumacare
AT haggardwarren developmentandevaluationofaninjectablechitosanbglycerophosphatepasteasalocalantibioticdeliverysystemfortraumacare
AT bumgardnerjoel developmentandevaluationofaninjectablechitosanbglycerophosphatepasteasalocalantibioticdeliverysystemfortraumacare
AT jenningsjessica developmentandevaluationofaninjectablechitosanbglycerophosphatepasteasalocalantibioticdeliverysystemfortraumacare