Cargando…

Comparison of Clinical Manifestations, Antimicrobial Susceptibility Patterns, and Mutations of Fluoroquinolone Target Genes between Elizabethkingia meningoseptica and Elizabethkingia anophelis Isolated in Taiwan

Elizabethkingia meningoseptica and Elizabethkingia anophelis are two major pathogens in the genus Elizabethkingia. Studies have revealed that Elizabethkingia anophelis is frequently misidentified as E. meningoseptica. Therefore, our aim was to explore the clinical and molecular differences between t...

Descripción completa

Detalles Bibliográficos
Autores principales: Lin, Jiun-Nong, Lai, Chung-Hsu, Yang, Chih-Hui, Huang, Yi-Han
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6306790/
https://www.ncbi.nlm.nih.gov/pubmed/30545016
http://dx.doi.org/10.3390/jcm7120538
_version_ 1783382857820930048
author Lin, Jiun-Nong
Lai, Chung-Hsu
Yang, Chih-Hui
Huang, Yi-Han
author_facet Lin, Jiun-Nong
Lai, Chung-Hsu
Yang, Chih-Hui
Huang, Yi-Han
author_sort Lin, Jiun-Nong
collection PubMed
description Elizabethkingia meningoseptica and Elizabethkingia anophelis are two major pathogens in the genus Elizabethkingia. Studies have revealed that Elizabethkingia anophelis is frequently misidentified as E. meningoseptica. Therefore, our aim was to explore the clinical and molecular differences between these two species. The database of a clinical microbiology laboratory in a university-affiliated hospital of Taiwan was searched to identify patients with Elizabethkingia infections between January 2005 and June 2018. Species were reidentified using 16S ribosomal RNA gene sequencing. Twenty E. meningoseptica and 72 E. anophelis samples were collected from consecutive patients. E. meningoseptica was significantly more frequently isolated from the cerebrospinal fluid than was E. anophelis. The most susceptible antibiotic for all Elizabethkingia isolates was minocycline (91.3%), followed by levofloxacin (52.2%), tigecycline (23.9%), and piperacillin tazobactam (23.9%). Compared with E. anophelis, E. meningoseptica was significantly less susceptible to piperacillin tazobactam, minocycline, and levofloxacin. Regarding nonsynonymous substitutions in the quinolone-resistance determining regions of DNA gyrase, six sites were recognized in E. meningoseptica and one site was recognized in E. anophelis. E. meningoseptica had a significantly higher rate of fluoroquinolone target gene mutations than did E. anophelis. Because of less susceptibility to multiple antibiotics than E. anophelis, empirical antimicrobial therapy of E. meningoseptica should be more rigorous.
format Online
Article
Text
id pubmed-6306790
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-63067902019-01-02 Comparison of Clinical Manifestations, Antimicrobial Susceptibility Patterns, and Mutations of Fluoroquinolone Target Genes between Elizabethkingia meningoseptica and Elizabethkingia anophelis Isolated in Taiwan Lin, Jiun-Nong Lai, Chung-Hsu Yang, Chih-Hui Huang, Yi-Han J Clin Med Article Elizabethkingia meningoseptica and Elizabethkingia anophelis are two major pathogens in the genus Elizabethkingia. Studies have revealed that Elizabethkingia anophelis is frequently misidentified as E. meningoseptica. Therefore, our aim was to explore the clinical and molecular differences between these two species. The database of a clinical microbiology laboratory in a university-affiliated hospital of Taiwan was searched to identify patients with Elizabethkingia infections between January 2005 and June 2018. Species were reidentified using 16S ribosomal RNA gene sequencing. Twenty E. meningoseptica and 72 E. anophelis samples were collected from consecutive patients. E. meningoseptica was significantly more frequently isolated from the cerebrospinal fluid than was E. anophelis. The most susceptible antibiotic for all Elizabethkingia isolates was minocycline (91.3%), followed by levofloxacin (52.2%), tigecycline (23.9%), and piperacillin tazobactam (23.9%). Compared with E. anophelis, E. meningoseptica was significantly less susceptible to piperacillin tazobactam, minocycline, and levofloxacin. Regarding nonsynonymous substitutions in the quinolone-resistance determining regions of DNA gyrase, six sites were recognized in E. meningoseptica and one site was recognized in E. anophelis. E. meningoseptica had a significantly higher rate of fluoroquinolone target gene mutations than did E. anophelis. Because of less susceptibility to multiple antibiotics than E. anophelis, empirical antimicrobial therapy of E. meningoseptica should be more rigorous. MDPI 2018-12-11 /pmc/articles/PMC6306790/ /pubmed/30545016 http://dx.doi.org/10.3390/jcm7120538 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lin, Jiun-Nong
Lai, Chung-Hsu
Yang, Chih-Hui
Huang, Yi-Han
Comparison of Clinical Manifestations, Antimicrobial Susceptibility Patterns, and Mutations of Fluoroquinolone Target Genes between Elizabethkingia meningoseptica and Elizabethkingia anophelis Isolated in Taiwan
title Comparison of Clinical Manifestations, Antimicrobial Susceptibility Patterns, and Mutations of Fluoroquinolone Target Genes between Elizabethkingia meningoseptica and Elizabethkingia anophelis Isolated in Taiwan
title_full Comparison of Clinical Manifestations, Antimicrobial Susceptibility Patterns, and Mutations of Fluoroquinolone Target Genes between Elizabethkingia meningoseptica and Elizabethkingia anophelis Isolated in Taiwan
title_fullStr Comparison of Clinical Manifestations, Antimicrobial Susceptibility Patterns, and Mutations of Fluoroquinolone Target Genes between Elizabethkingia meningoseptica and Elizabethkingia anophelis Isolated in Taiwan
title_full_unstemmed Comparison of Clinical Manifestations, Antimicrobial Susceptibility Patterns, and Mutations of Fluoroquinolone Target Genes between Elizabethkingia meningoseptica and Elizabethkingia anophelis Isolated in Taiwan
title_short Comparison of Clinical Manifestations, Antimicrobial Susceptibility Patterns, and Mutations of Fluoroquinolone Target Genes between Elizabethkingia meningoseptica and Elizabethkingia anophelis Isolated in Taiwan
title_sort comparison of clinical manifestations, antimicrobial susceptibility patterns, and mutations of fluoroquinolone target genes between elizabethkingia meningoseptica and elizabethkingia anophelis isolated in taiwan
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6306790/
https://www.ncbi.nlm.nih.gov/pubmed/30545016
http://dx.doi.org/10.3390/jcm7120538
work_keys_str_mv AT linjiunnong comparisonofclinicalmanifestationsantimicrobialsusceptibilitypatternsandmutationsoffluoroquinolonetargetgenesbetweenelizabethkingiameningosepticaandelizabethkingiaanophelisisolatedintaiwan
AT laichunghsu comparisonofclinicalmanifestationsantimicrobialsusceptibilitypatternsandmutationsoffluoroquinolonetargetgenesbetweenelizabethkingiameningosepticaandelizabethkingiaanophelisisolatedintaiwan
AT yangchihhui comparisonofclinicalmanifestationsantimicrobialsusceptibilitypatternsandmutationsoffluoroquinolonetargetgenesbetweenelizabethkingiameningosepticaandelizabethkingiaanophelisisolatedintaiwan
AT huangyihan comparisonofclinicalmanifestationsantimicrobialsusceptibilitypatternsandmutationsoffluoroquinolonetargetgenesbetweenelizabethkingiameningosepticaandelizabethkingiaanophelisisolatedintaiwan