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Three-Dimensional Bone Substitutes for Oral and Maxillofacial Surgery: Biological and Structural Characterization

Background: Bone substitutes, either from human (autografts and allografts) or animal (xenografts) sources, suffer from inherent drawbacks including limited availability or potential infectivity to name a few. In the last decade, synthetic biomaterials have emerged as a valid alternative for biomedi...

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Autores principales: Turco, Gianluca, Porrelli, Davide, Marsich, Eleonora, Vecchies, Federica, Lombardi, Teresa, Stacchi, Claudio, Di Lenarda, Roberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6306815/
https://www.ncbi.nlm.nih.gov/pubmed/30413004
http://dx.doi.org/10.3390/jfb9040062
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author Turco, Gianluca
Porrelli, Davide
Marsich, Eleonora
Vecchies, Federica
Lombardi, Teresa
Stacchi, Claudio
Di Lenarda, Roberto
author_facet Turco, Gianluca
Porrelli, Davide
Marsich, Eleonora
Vecchies, Federica
Lombardi, Teresa
Stacchi, Claudio
Di Lenarda, Roberto
author_sort Turco, Gianluca
collection PubMed
description Background: Bone substitutes, either from human (autografts and allografts) or animal (xenografts) sources, suffer from inherent drawbacks including limited availability or potential infectivity to name a few. In the last decade, synthetic biomaterials have emerged as a valid alternative for biomedical applications in the field of orthopedic and maxillofacial surgery. In particular, phosphate-based bone substitution materials have exhibited a high biocompatibility due to their chemical similitude with natural hydroxyapatite. Besides the nature of the biomaterial, its porous and interconnected architecture is essential for a correct osseointegration. This performance could be predicted with an extensive characterization of the biomaterial in vitro. Methods: In this study, we compared the biological, chemical, and structural features of four different commercially available bone substitutes derived from an animal or a synthetic source. To this end, µ-CT and SEM were used to describe the biomaterials structure. Both FTIR and EDS analyses were carried out to provide a chemical characterization. The results obtained by these techniques were correlated with cell adhesion and proliferation of the osteosarcoma MG-63 human cell line cultured in vitro. Results: The findings reported in this paper indicate a significant influence of both the nature and the structure of the biomaterials in cell adhesion and proliferation, which ultimately could affect the clinical performance of the biomaterials. Conclusions: The four commercially available bone substitutes investigated in this work significantly differed in terms of structural features, which ultimately influenced in vitro cell proliferation and may so affect the clinical performance of the biomaterials.
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spelling pubmed-63068152019-01-02 Three-Dimensional Bone Substitutes for Oral and Maxillofacial Surgery: Biological and Structural Characterization Turco, Gianluca Porrelli, Davide Marsich, Eleonora Vecchies, Federica Lombardi, Teresa Stacchi, Claudio Di Lenarda, Roberto J Funct Biomater Article Background: Bone substitutes, either from human (autografts and allografts) or animal (xenografts) sources, suffer from inherent drawbacks including limited availability or potential infectivity to name a few. In the last decade, synthetic biomaterials have emerged as a valid alternative for biomedical applications in the field of orthopedic and maxillofacial surgery. In particular, phosphate-based bone substitution materials have exhibited a high biocompatibility due to their chemical similitude with natural hydroxyapatite. Besides the nature of the biomaterial, its porous and interconnected architecture is essential for a correct osseointegration. This performance could be predicted with an extensive characterization of the biomaterial in vitro. Methods: In this study, we compared the biological, chemical, and structural features of four different commercially available bone substitutes derived from an animal or a synthetic source. To this end, µ-CT and SEM were used to describe the biomaterials structure. Both FTIR and EDS analyses were carried out to provide a chemical characterization. The results obtained by these techniques were correlated with cell adhesion and proliferation of the osteosarcoma MG-63 human cell line cultured in vitro. Results: The findings reported in this paper indicate a significant influence of both the nature and the structure of the biomaterials in cell adhesion and proliferation, which ultimately could affect the clinical performance of the biomaterials. Conclusions: The four commercially available bone substitutes investigated in this work significantly differed in terms of structural features, which ultimately influenced in vitro cell proliferation and may so affect the clinical performance of the biomaterials. MDPI 2018-11-08 /pmc/articles/PMC6306815/ /pubmed/30413004 http://dx.doi.org/10.3390/jfb9040062 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Turco, Gianluca
Porrelli, Davide
Marsich, Eleonora
Vecchies, Federica
Lombardi, Teresa
Stacchi, Claudio
Di Lenarda, Roberto
Three-Dimensional Bone Substitutes for Oral and Maxillofacial Surgery: Biological and Structural Characterization
title Three-Dimensional Bone Substitutes for Oral and Maxillofacial Surgery: Biological and Structural Characterization
title_full Three-Dimensional Bone Substitutes for Oral and Maxillofacial Surgery: Biological and Structural Characterization
title_fullStr Three-Dimensional Bone Substitutes for Oral and Maxillofacial Surgery: Biological and Structural Characterization
title_full_unstemmed Three-Dimensional Bone Substitutes for Oral and Maxillofacial Surgery: Biological and Structural Characterization
title_short Three-Dimensional Bone Substitutes for Oral and Maxillofacial Surgery: Biological and Structural Characterization
title_sort three-dimensional bone substitutes for oral and maxillofacial surgery: biological and structural characterization
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6306815/
https://www.ncbi.nlm.nih.gov/pubmed/30413004
http://dx.doi.org/10.3390/jfb9040062
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