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Assessment of a Rabies Virus Rapid Diagnostic Test for the Detection of Australian Bat Lyssavirus

Australian bat lyssavirus (ABLV) is closely related to the classical rabies virus and has been associated with three human fatalities and two equine fatalities in Australia. ABLV infection in humans causes encephalomyelitis, resulting in fatal disease, but has no effective therapy. The virus is main...

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Autores principales: Certoma, Andrea, Lunt, Ross A., Vosloo, Wilna, Smith, Ina, Colling, Axel, Williams, David T., Tran, Thao, Blacksell, Stuart D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6306826/
https://www.ncbi.nlm.nih.gov/pubmed/30287778
http://dx.doi.org/10.3390/tropicalmed3040109
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author Certoma, Andrea
Lunt, Ross A.
Vosloo, Wilna
Smith, Ina
Colling, Axel
Williams, David T.
Tran, Thao
Blacksell, Stuart D.
author_facet Certoma, Andrea
Lunt, Ross A.
Vosloo, Wilna
Smith, Ina
Colling, Axel
Williams, David T.
Tran, Thao
Blacksell, Stuart D.
author_sort Certoma, Andrea
collection PubMed
description Australian bat lyssavirus (ABLV) is closely related to the classical rabies virus and has been associated with three human fatalities and two equine fatalities in Australia. ABLV infection in humans causes encephalomyelitis, resulting in fatal disease, but has no effective therapy. The virus is maintained in enzootic circulation within fruit bats (Pteropid spp.) and at least one insectivorous bat variety (Saccolaimus flaviventris). Most frequently, laboratory testing is conducted on pteropodid bat brains, either following a potential human exposure through bites, scratches and other direct contacts with bats, or as opportunistic assessment of sick or dead bats. The level of medical intervention and post-exposure prophylaxis is largely determined on laboratory testing for antigen/virus as the demonstrable infection status of the in-contact bat. This study evaluates the comparative diagnostic performance of a lateral flow test, Anigen Rabies Ag detection rapid test (RDT), in pteropodid variant of ABLV-infected bat brain tissues. The RDT demonstrated 100% agreement with the reference standard fluorescent antibody test on 43 clinical samples suggesting a potential application in rapid diagnosis of pteropodid variant of ABLV infection. A weighted Kappa value of 0.95 confirmed a high level of agreement between both tests.
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spelling pubmed-63068262019-01-02 Assessment of a Rabies Virus Rapid Diagnostic Test for the Detection of Australian Bat Lyssavirus Certoma, Andrea Lunt, Ross A. Vosloo, Wilna Smith, Ina Colling, Axel Williams, David T. Tran, Thao Blacksell, Stuart D. Trop Med Infect Dis Article Australian bat lyssavirus (ABLV) is closely related to the classical rabies virus and has been associated with three human fatalities and two equine fatalities in Australia. ABLV infection in humans causes encephalomyelitis, resulting in fatal disease, but has no effective therapy. The virus is maintained in enzootic circulation within fruit bats (Pteropid spp.) and at least one insectivorous bat variety (Saccolaimus flaviventris). Most frequently, laboratory testing is conducted on pteropodid bat brains, either following a potential human exposure through bites, scratches and other direct contacts with bats, or as opportunistic assessment of sick or dead bats. The level of medical intervention and post-exposure prophylaxis is largely determined on laboratory testing for antigen/virus as the demonstrable infection status of the in-contact bat. This study evaluates the comparative diagnostic performance of a lateral flow test, Anigen Rabies Ag detection rapid test (RDT), in pteropodid variant of ABLV-infected bat brain tissues. The RDT demonstrated 100% agreement with the reference standard fluorescent antibody test on 43 clinical samples suggesting a potential application in rapid diagnosis of pteropodid variant of ABLV infection. A weighted Kappa value of 0.95 confirmed a high level of agreement between both tests. MDPI 2018-10-04 /pmc/articles/PMC6306826/ /pubmed/30287778 http://dx.doi.org/10.3390/tropicalmed3040109 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Certoma, Andrea
Lunt, Ross A.
Vosloo, Wilna
Smith, Ina
Colling, Axel
Williams, David T.
Tran, Thao
Blacksell, Stuart D.
Assessment of a Rabies Virus Rapid Diagnostic Test for the Detection of Australian Bat Lyssavirus
title Assessment of a Rabies Virus Rapid Diagnostic Test for the Detection of Australian Bat Lyssavirus
title_full Assessment of a Rabies Virus Rapid Diagnostic Test for the Detection of Australian Bat Lyssavirus
title_fullStr Assessment of a Rabies Virus Rapid Diagnostic Test for the Detection of Australian Bat Lyssavirus
title_full_unstemmed Assessment of a Rabies Virus Rapid Diagnostic Test for the Detection of Australian Bat Lyssavirus
title_short Assessment of a Rabies Virus Rapid Diagnostic Test for the Detection of Australian Bat Lyssavirus
title_sort assessment of a rabies virus rapid diagnostic test for the detection of australian bat lyssavirus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6306826/
https://www.ncbi.nlm.nih.gov/pubmed/30287778
http://dx.doi.org/10.3390/tropicalmed3040109
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