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Zika Virus Epidemic in Brazil. II. Post-Mortem Analyses of Neonates with Microcephaly, Stillbirths, and Miscarriage

Introduction: The recent Zika virus(ZIKV) epidemic in Brazil was characterized by a range of different clinical presentations, particularly microcephaly, Guillain-Barré syndrome, and death. In this context, we determined the causal relationship between fatal microcephaly cases and ZIKV infection. Me...

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Autores principales: Azevedo, Raimunda S. S., Araujo, Marialva T., Oliveira, Consuelo S., Martins Filho, Arnaldo Jorge, Nunes, Bruno T. D., Henriques, Daniele F., Silva, Eliana V. P., Carvalho, Valéria L., Chiang, Jannifer O., Martins, Lívia C., Vasconcelos, Barbara C. B., Sousa, Jorge R., Araujo, Fernanda Montenegro C., Ribeiro, Erlane M., Castro, Andrezza R. P., de Queiroz, Maria G. L., Verotti, Mariana P., Nunes, Márcio R. T., Cruz, Ana C. R., Rodrigues, Sueli G., Shi, Pei-Yong, Quaresma, Juarez A. S., Tesh, Robert B., Vasconcelos, Pedro F. C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6306831/
https://www.ncbi.nlm.nih.gov/pubmed/30487475
http://dx.doi.org/10.3390/jcm7120496
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author Azevedo, Raimunda S. S.
Araujo, Marialva T.
Oliveira, Consuelo S.
Martins Filho, Arnaldo Jorge
Nunes, Bruno T. D.
Henriques, Daniele F.
Silva, Eliana V. P.
Carvalho, Valéria L.
Chiang, Jannifer O.
Martins, Lívia C.
Vasconcelos, Barbara C. B.
Sousa, Jorge R.
Araujo, Fernanda Montenegro C.
Ribeiro, Erlane M.
Castro, Andrezza R. P.
de Queiroz, Maria G. L.
Verotti, Mariana P.
Nunes, Márcio R. T.
Cruz, Ana C. R.
Rodrigues, Sueli G.
Shi, Pei-Yong
Quaresma, Juarez A. S.
Tesh, Robert B.
Vasconcelos, Pedro F. C.
author_facet Azevedo, Raimunda S. S.
Araujo, Marialva T.
Oliveira, Consuelo S.
Martins Filho, Arnaldo Jorge
Nunes, Bruno T. D.
Henriques, Daniele F.
Silva, Eliana V. P.
Carvalho, Valéria L.
Chiang, Jannifer O.
Martins, Lívia C.
Vasconcelos, Barbara C. B.
Sousa, Jorge R.
Araujo, Fernanda Montenegro C.
Ribeiro, Erlane M.
Castro, Andrezza R. P.
de Queiroz, Maria G. L.
Verotti, Mariana P.
Nunes, Márcio R. T.
Cruz, Ana C. R.
Rodrigues, Sueli G.
Shi, Pei-Yong
Quaresma, Juarez A. S.
Tesh, Robert B.
Vasconcelos, Pedro F. C.
author_sort Azevedo, Raimunda S. S.
collection PubMed
description Introduction: The recent Zika virus(ZIKV) epidemic in Brazil was characterized by a range of different clinical presentations, particularly microcephaly, Guillain-Barré syndrome, and death. In this context, we determined the causal relationship between fatal microcephaly cases and ZIKV infection. Methods: Twelve fatal cases of neonates, whose mothers were infected with ZIKV during pregnancy, were examined; cases included nine neonatal deaths due to microcephaly, one miscarriage, and two stillbirths. Tissue samples were obtained from all cases at necropsy and were submitted for virological investigation (RT-qPCR and virus isolation) and/or histopathology (hematoxylin and eosin staining) and immunohistochemical assay for the detection of ZIKV antigens. Results: ZIKV antigens and/or ZIKV RNA were detected in tissue samples of all 12 cases examined. ZIKV was recovered in one case. Results of the virological and immunohistochemical analyses, as well as the anatomic abnormalities and histopathologic changes observed at necropsy on the 12 fatal cases, are presented. Conclusions: Data from these 12 cases provide strong evidence of the causal relationship between ZIKV and congenital disease in fetuses of women who were infected with the virus during pregnancy.
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spelling pubmed-63068312019-01-02 Zika Virus Epidemic in Brazil. II. Post-Mortem Analyses of Neonates with Microcephaly, Stillbirths, and Miscarriage Azevedo, Raimunda S. S. Araujo, Marialva T. Oliveira, Consuelo S. Martins Filho, Arnaldo Jorge Nunes, Bruno T. D. Henriques, Daniele F. Silva, Eliana V. P. Carvalho, Valéria L. Chiang, Jannifer O. Martins, Lívia C. Vasconcelos, Barbara C. B. Sousa, Jorge R. Araujo, Fernanda Montenegro C. Ribeiro, Erlane M. Castro, Andrezza R. P. de Queiroz, Maria G. L. Verotti, Mariana P. Nunes, Márcio R. T. Cruz, Ana C. R. Rodrigues, Sueli G. Shi, Pei-Yong Quaresma, Juarez A. S. Tesh, Robert B. Vasconcelos, Pedro F. C. J Clin Med Article Introduction: The recent Zika virus(ZIKV) epidemic in Brazil was characterized by a range of different clinical presentations, particularly microcephaly, Guillain-Barré syndrome, and death. In this context, we determined the causal relationship between fatal microcephaly cases and ZIKV infection. Methods: Twelve fatal cases of neonates, whose mothers were infected with ZIKV during pregnancy, were examined; cases included nine neonatal deaths due to microcephaly, one miscarriage, and two stillbirths. Tissue samples were obtained from all cases at necropsy and were submitted for virological investigation (RT-qPCR and virus isolation) and/or histopathology (hematoxylin and eosin staining) and immunohistochemical assay for the detection of ZIKV antigens. Results: ZIKV antigens and/or ZIKV RNA were detected in tissue samples of all 12 cases examined. ZIKV was recovered in one case. Results of the virological and immunohistochemical analyses, as well as the anatomic abnormalities and histopathologic changes observed at necropsy on the 12 fatal cases, are presented. Conclusions: Data from these 12 cases provide strong evidence of the causal relationship between ZIKV and congenital disease in fetuses of women who were infected with the virus during pregnancy. MDPI 2018-11-28 /pmc/articles/PMC6306831/ /pubmed/30487475 http://dx.doi.org/10.3390/jcm7120496 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Azevedo, Raimunda S. S.
Araujo, Marialva T.
Oliveira, Consuelo S.
Martins Filho, Arnaldo Jorge
Nunes, Bruno T. D.
Henriques, Daniele F.
Silva, Eliana V. P.
Carvalho, Valéria L.
Chiang, Jannifer O.
Martins, Lívia C.
Vasconcelos, Barbara C. B.
Sousa, Jorge R.
Araujo, Fernanda Montenegro C.
Ribeiro, Erlane M.
Castro, Andrezza R. P.
de Queiroz, Maria G. L.
Verotti, Mariana P.
Nunes, Márcio R. T.
Cruz, Ana C. R.
Rodrigues, Sueli G.
Shi, Pei-Yong
Quaresma, Juarez A. S.
Tesh, Robert B.
Vasconcelos, Pedro F. C.
Zika Virus Epidemic in Brazil. II. Post-Mortem Analyses of Neonates with Microcephaly, Stillbirths, and Miscarriage
title Zika Virus Epidemic in Brazil. II. Post-Mortem Analyses of Neonates with Microcephaly, Stillbirths, and Miscarriage
title_full Zika Virus Epidemic in Brazil. II. Post-Mortem Analyses of Neonates with Microcephaly, Stillbirths, and Miscarriage
title_fullStr Zika Virus Epidemic in Brazil. II. Post-Mortem Analyses of Neonates with Microcephaly, Stillbirths, and Miscarriage
title_full_unstemmed Zika Virus Epidemic in Brazil. II. Post-Mortem Analyses of Neonates with Microcephaly, Stillbirths, and Miscarriage
title_short Zika Virus Epidemic in Brazil. II. Post-Mortem Analyses of Neonates with Microcephaly, Stillbirths, and Miscarriage
title_sort zika virus epidemic in brazil. ii. post-mortem analyses of neonates with microcephaly, stillbirths, and miscarriage
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6306831/
https://www.ncbi.nlm.nih.gov/pubmed/30487475
http://dx.doi.org/10.3390/jcm7120496
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