Cargando…

Bone Immune Response to Materials, Part I: Titanium, PEEK and Copper in Comparison to Sham at 10 Days in Rabbit Tibia

Bone anchored biomaterials have become an indispensable solution for the restoration of lost dental elements and for skeletal joint replacements. However, a thorough understanding is still lacking in terms of the biological mechanisms leading to osseointegration and its contrast, unwanted peri-impla...

Descripción completa

Detalles Bibliográficos
Autores principales: Trindade, Ricardo, Albrektsson, Tomas, Galli, Silvia, Prgomet, Zdenka, Tengvall, Pentti, Wennerberg, Ann
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6307090/
https://www.ncbi.nlm.nih.gov/pubmed/30544551
http://dx.doi.org/10.3390/jcm7120526
_version_ 1783382926167113728
author Trindade, Ricardo
Albrektsson, Tomas
Galli, Silvia
Prgomet, Zdenka
Tengvall, Pentti
Wennerberg, Ann
author_facet Trindade, Ricardo
Albrektsson, Tomas
Galli, Silvia
Prgomet, Zdenka
Tengvall, Pentti
Wennerberg, Ann
author_sort Trindade, Ricardo
collection PubMed
description Bone anchored biomaterials have become an indispensable solution for the restoration of lost dental elements and for skeletal joint replacements. However, a thorough understanding is still lacking in terms of the biological mechanisms leading to osseointegration and its contrast, unwanted peri-implant bone loss. We have previously hypothesized on the participation of immune mechanisms in such processes, and later demonstrated enhanced bone immune activation up to 4 weeks around titanium implants. The current experimental study explored and compared in a rabbit tibia model after 10 days of healing time, the bone inflammation/immunological reaction at mRNA level towards titanium, polyether ether ketone (PEEK) and copper compared to a Sham control. Samples from the test and control sites were, after a healing period, processed for gene expression analysis (polymerase chain reaction, (qPCR)) and decalcified histology tissue analysis. All materials displayed immune activation and suppression of bone resorption, when compared to sham. The M1 (inflammatory)/M2 (reparative) -macrophage phenotype balance was correlated to the proximity and volume of bone growth at the implant vicinity, with titanium demonstrating a M2-phenotype at 10 days, whereas copper and PEEK were still dealing with a mixed M1- and M2-phenotype environment. Titanium was the only material showing adequate bone growth and proximity inside the implant threads. There was a consistent upregulation of (T-cell surface glycoprotein CD4) CD4 and downregulation of (T-cell transmembrane glycoprotein CD8) CD8, indicating a CD4-lymphocyte phenotype driven reaction around all materials at 10 days.
format Online
Article
Text
id pubmed-6307090
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-63070902019-01-02 Bone Immune Response to Materials, Part I: Titanium, PEEK and Copper in Comparison to Sham at 10 Days in Rabbit Tibia Trindade, Ricardo Albrektsson, Tomas Galli, Silvia Prgomet, Zdenka Tengvall, Pentti Wennerberg, Ann J Clin Med Article Bone anchored biomaterials have become an indispensable solution for the restoration of lost dental elements and for skeletal joint replacements. However, a thorough understanding is still lacking in terms of the biological mechanisms leading to osseointegration and its contrast, unwanted peri-implant bone loss. We have previously hypothesized on the participation of immune mechanisms in such processes, and later demonstrated enhanced bone immune activation up to 4 weeks around titanium implants. The current experimental study explored and compared in a rabbit tibia model after 10 days of healing time, the bone inflammation/immunological reaction at mRNA level towards titanium, polyether ether ketone (PEEK) and copper compared to a Sham control. Samples from the test and control sites were, after a healing period, processed for gene expression analysis (polymerase chain reaction, (qPCR)) and decalcified histology tissue analysis. All materials displayed immune activation and suppression of bone resorption, when compared to sham. The M1 (inflammatory)/M2 (reparative) -macrophage phenotype balance was correlated to the proximity and volume of bone growth at the implant vicinity, with titanium demonstrating a M2-phenotype at 10 days, whereas copper and PEEK were still dealing with a mixed M1- and M2-phenotype environment. Titanium was the only material showing adequate bone growth and proximity inside the implant threads. There was a consistent upregulation of (T-cell surface glycoprotein CD4) CD4 and downregulation of (T-cell transmembrane glycoprotein CD8) CD8, indicating a CD4-lymphocyte phenotype driven reaction around all materials at 10 days. MDPI 2018-12-07 /pmc/articles/PMC6307090/ /pubmed/30544551 http://dx.doi.org/10.3390/jcm7120526 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Trindade, Ricardo
Albrektsson, Tomas
Galli, Silvia
Prgomet, Zdenka
Tengvall, Pentti
Wennerberg, Ann
Bone Immune Response to Materials, Part I: Titanium, PEEK and Copper in Comparison to Sham at 10 Days in Rabbit Tibia
title Bone Immune Response to Materials, Part I: Titanium, PEEK and Copper in Comparison to Sham at 10 Days in Rabbit Tibia
title_full Bone Immune Response to Materials, Part I: Titanium, PEEK and Copper in Comparison to Sham at 10 Days in Rabbit Tibia
title_fullStr Bone Immune Response to Materials, Part I: Titanium, PEEK and Copper in Comparison to Sham at 10 Days in Rabbit Tibia
title_full_unstemmed Bone Immune Response to Materials, Part I: Titanium, PEEK and Copper in Comparison to Sham at 10 Days in Rabbit Tibia
title_short Bone Immune Response to Materials, Part I: Titanium, PEEK and Copper in Comparison to Sham at 10 Days in Rabbit Tibia
title_sort bone immune response to materials, part i: titanium, peek and copper in comparison to sham at 10 days in rabbit tibia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6307090/
https://www.ncbi.nlm.nih.gov/pubmed/30544551
http://dx.doi.org/10.3390/jcm7120526
work_keys_str_mv AT trindadericardo boneimmuneresponsetomaterialspartititaniumpeekandcopperincomparisontoshamat10daysinrabbittibia
AT albrektssontomas boneimmuneresponsetomaterialspartititaniumpeekandcopperincomparisontoshamat10daysinrabbittibia
AT gallisilvia boneimmuneresponsetomaterialspartititaniumpeekandcopperincomparisontoshamat10daysinrabbittibia
AT prgometzdenka boneimmuneresponsetomaterialspartititaniumpeekandcopperincomparisontoshamat10daysinrabbittibia
AT tengvallpentti boneimmuneresponsetomaterialspartititaniumpeekandcopperincomparisontoshamat10daysinrabbittibia
AT wennerbergann boneimmuneresponsetomaterialspartititaniumpeekandcopperincomparisontoshamat10daysinrabbittibia