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A Simple Cloning-free Method to Efficiently Induce Gene Expression Using CRISPR/Cas9
Gain-of-function studies often require the tedious cloning of transgene cDNA into vectors for overexpression beyond the physiological expression levels. The rapid development of CRISPR/Cas technology presents promising opportunities to address these issues. Here, we report a simple, cloning-free met...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society of Gene & Cell Therapy
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6307107/ https://www.ncbi.nlm.nih.gov/pubmed/30594894 http://dx.doi.org/10.1016/j.omtn.2018.11.008 |