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Combination of ELISA screening and seroneutralisation tests to expedite Zika virus seroprevalence studies

Here we propose a strategy allowing implementing efficient and practicable large-scale seroepidemiological studies for Zika Virus (ZIKV). It combines screening by a commercial NS1 protein-based Zika IgG ELISA, and confirmation by a cytopathic effect-based virus neutralization test (CPE-based VNT). I...

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Autores principales: Nurtop, Elif, Villarroel, Paola Mariela Saba, Pastorino, Boris, Ninove, Laetitia, Drexler, Jan-Felix, Roca, Yelin, Gake, Bouba, Dubot-Peres, Audrey, Grard, Gilda, Peyrefitte, Christophe, Priet, Stéphane, de Lamballerie, Xavier, Gallian, Pierre
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6307276/
https://www.ncbi.nlm.nih.gov/pubmed/30587193
http://dx.doi.org/10.1186/s12985-018-1105-5
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author Nurtop, Elif
Villarroel, Paola Mariela Saba
Pastorino, Boris
Ninove, Laetitia
Drexler, Jan-Felix
Roca, Yelin
Gake, Bouba
Dubot-Peres, Audrey
Grard, Gilda
Peyrefitte, Christophe
Priet, Stéphane
de Lamballerie, Xavier
Gallian, Pierre
author_facet Nurtop, Elif
Villarroel, Paola Mariela Saba
Pastorino, Boris
Ninove, Laetitia
Drexler, Jan-Felix
Roca, Yelin
Gake, Bouba
Dubot-Peres, Audrey
Grard, Gilda
Peyrefitte, Christophe
Priet, Stéphane
de Lamballerie, Xavier
Gallian, Pierre
author_sort Nurtop, Elif
collection PubMed
description Here we propose a strategy allowing implementing efficient and practicable large-scale seroepidemiological studies for Zika Virus (ZIKV). It combines screening by a commercial NS1 protein-based Zika IgG ELISA, and confirmation by a cytopathic effect-based virus neutralization test (CPE-based VNT). In post-epidemic samples from Martinique Island blood donors (a population with a dengue seroprevalence above 90%), this strategy allowed reaching specificity and sensitivity values over 98%. The CPE-based VNT consists of recording CPE directly under the optical microscope, which is easy to identify with ZIKV strain H/PF/2013 at day 5 pi. Overall, considered that CPE-based VNT is cost effective and widely automatable, the NS1 protein-based Zika IgG ELISA+CPE-based VNT combination strategy represents a convenient tool to expedite ZIKV seroprevalence studies. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12985-018-1105-5) contains supplementary material, which is available to authorized users.
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spelling pubmed-63072762019-01-02 Combination of ELISA screening and seroneutralisation tests to expedite Zika virus seroprevalence studies Nurtop, Elif Villarroel, Paola Mariela Saba Pastorino, Boris Ninove, Laetitia Drexler, Jan-Felix Roca, Yelin Gake, Bouba Dubot-Peres, Audrey Grard, Gilda Peyrefitte, Christophe Priet, Stéphane de Lamballerie, Xavier Gallian, Pierre Virol J Short Report Here we propose a strategy allowing implementing efficient and practicable large-scale seroepidemiological studies for Zika Virus (ZIKV). It combines screening by a commercial NS1 protein-based Zika IgG ELISA, and confirmation by a cytopathic effect-based virus neutralization test (CPE-based VNT). In post-epidemic samples from Martinique Island blood donors (a population with a dengue seroprevalence above 90%), this strategy allowed reaching specificity and sensitivity values over 98%. The CPE-based VNT consists of recording CPE directly under the optical microscope, which is easy to identify with ZIKV strain H/PF/2013 at day 5 pi. Overall, considered that CPE-based VNT is cost effective and widely automatable, the NS1 protein-based Zika IgG ELISA+CPE-based VNT combination strategy represents a convenient tool to expedite ZIKV seroprevalence studies. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12985-018-1105-5) contains supplementary material, which is available to authorized users. BioMed Central 2018-12-27 /pmc/articles/PMC6307276/ /pubmed/30587193 http://dx.doi.org/10.1186/s12985-018-1105-5 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Report
Nurtop, Elif
Villarroel, Paola Mariela Saba
Pastorino, Boris
Ninove, Laetitia
Drexler, Jan-Felix
Roca, Yelin
Gake, Bouba
Dubot-Peres, Audrey
Grard, Gilda
Peyrefitte, Christophe
Priet, Stéphane
de Lamballerie, Xavier
Gallian, Pierre
Combination of ELISA screening and seroneutralisation tests to expedite Zika virus seroprevalence studies
title Combination of ELISA screening and seroneutralisation tests to expedite Zika virus seroprevalence studies
title_full Combination of ELISA screening and seroneutralisation tests to expedite Zika virus seroprevalence studies
title_fullStr Combination of ELISA screening and seroneutralisation tests to expedite Zika virus seroprevalence studies
title_full_unstemmed Combination of ELISA screening and seroneutralisation tests to expedite Zika virus seroprevalence studies
title_short Combination of ELISA screening and seroneutralisation tests to expedite Zika virus seroprevalence studies
title_sort combination of elisa screening and seroneutralisation tests to expedite zika virus seroprevalence studies
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6307276/
https://www.ncbi.nlm.nih.gov/pubmed/30587193
http://dx.doi.org/10.1186/s12985-018-1105-5
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