Nitric Oxide Production from Nitrite Reduction and Hydroxylamine Oxidation by Copper-containing Dissimilatory Nitrite Reductase (NirK) from the Aerobic Ammonia-oxidizing Archaeon, Nitrososphaera viennensis

Aerobic ammonia-oxidizing archaea (AOA) play a crucial role in the global nitrogen cycle by oxidizing ammonia to nitrite, and nitric oxide (NO) is a key intermediate in AOA for sustaining aerobic ammonia oxidation activity. We herein heterologously expressed the NO-forming, copper-containing, dissim...

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Detalles Bibliográficos
Autores principales: Kobayashi, Shun, Hira, Daisuke, Yoshida, Keitaro, Toyofuku, Masanori, Shida, Yosuke, Ogasawara, Wataru, Yamaguchi, Takashi, Araki, Nobuo, Oshiki, Mamoru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: the Japanese Society of Microbial Ecology (JSME)/the Japanese Society of Soil Microbiology (JSSM)/the Taiwan Society of Microbial Ecology (TSME)/the Japanese Society of Plant Microbe Interactions (JSPMI) 2018
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6308003/
https://www.ncbi.nlm.nih.gov/pubmed/30318500
http://dx.doi.org/10.1264/jsme2.ME18058
Descripción
Sumario:Aerobic ammonia-oxidizing archaea (AOA) play a crucial role in the global nitrogen cycle by oxidizing ammonia to nitrite, and nitric oxide (NO) is a key intermediate in AOA for sustaining aerobic ammonia oxidation activity. We herein heterologously expressed the NO-forming, copper-containing, dissimilatory nitrite reductase (NirK) from Nitrososphaera viennensis and investigated its enzymatic properties. The recombinant protein catalyzed the reduction of (15)NO(2)(−) to (15)NO, the oxidation of hydroxylamine ((15)NH(2)OH) to (15)NO, and the production of (14–15)N(2)O from (15)NH(2)OH and (14)NO(2)(−). To the best of our knowledge, the present study is the first to document the enzymatic properties of AOA NirK.

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