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Recovery rates of combination antibiotic therapy using in vitro microdialysis simulating in vivo conditions

Microdialysis is a technique used to measure the unbound antibiotic concentration in the interstitial spaces, the target site of action. In vitro recovery studies are essential to calibrating the microdialysis system for in vivo studies. The effect of a combination of antibiotics on recovery into mi...

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Autores principales: Dhanani, Jayesh A., Parker, Suzanne L., Lipman, Jeffrey, Wallis, Steven C., Cohen, Jeremy, Fraser, John, Barnett, Adrian, Chew, Michelle, Roberts, Jason A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Xi'an Jiaotong University 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6308031/
https://www.ncbi.nlm.nih.gov/pubmed/30595948
http://dx.doi.org/10.1016/j.jpha.2018.07.003
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author Dhanani, Jayesh A.
Parker, Suzanne L.
Lipman, Jeffrey
Wallis, Steven C.
Cohen, Jeremy
Fraser, John
Barnett, Adrian
Chew, Michelle
Roberts, Jason A.
author_facet Dhanani, Jayesh A.
Parker, Suzanne L.
Lipman, Jeffrey
Wallis, Steven C.
Cohen, Jeremy
Fraser, John
Barnett, Adrian
Chew, Michelle
Roberts, Jason A.
author_sort Dhanani, Jayesh A.
collection PubMed
description Microdialysis is a technique used to measure the unbound antibiotic concentration in the interstitial spaces, the target site of action. In vitro recovery studies are essential to calibrating the microdialysis system for in vivo studies. The effect of a combination of antibiotics on recovery into microdialysate requires investigation. In vitro microdialysis recovery studies were conducted on a combination of vancomycin and tobramycin, in a simulated in vivo model. Comparison was made between recoveries for three different concentrations and three different perfusate flow rates. The overall relative recovery for vancomycin was lower than that of tobramycin. For tobramycin, a concentration of 20μg/mL and flow rate of 1.0μL/min had the best recovery. A concentration of 5.0μg/mL and flow rate of 1.0μL/min yielded maximal recovery for vancomycin. Large molecular size and higher protein binding resulted in lower relative recoveries for vancomycin. Perfusate flow rates and drug concentrations affected the relative recovery when a combination of vancomycin and tobramycin was tested. Low perfusate flow rates were associated with higher recovery rates. For combination antibiotic measurement which includes agents that are highly protein bound, in vitro studies performed prior to in vivo studies may ensure the reliable measurement of unbound concentrations.
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spelling pubmed-63080312018-12-28 Recovery rates of combination antibiotic therapy using in vitro microdialysis simulating in vivo conditions Dhanani, Jayesh A. Parker, Suzanne L. Lipman, Jeffrey Wallis, Steven C. Cohen, Jeremy Fraser, John Barnett, Adrian Chew, Michelle Roberts, Jason A. J Pharm Anal Original Article Microdialysis is a technique used to measure the unbound antibiotic concentration in the interstitial spaces, the target site of action. In vitro recovery studies are essential to calibrating the microdialysis system for in vivo studies. The effect of a combination of antibiotics on recovery into microdialysate requires investigation. In vitro microdialysis recovery studies were conducted on a combination of vancomycin and tobramycin, in a simulated in vivo model. Comparison was made between recoveries for three different concentrations and three different perfusate flow rates. The overall relative recovery for vancomycin was lower than that of tobramycin. For tobramycin, a concentration of 20μg/mL and flow rate of 1.0μL/min had the best recovery. A concentration of 5.0μg/mL and flow rate of 1.0μL/min yielded maximal recovery for vancomycin. Large molecular size and higher protein binding resulted in lower relative recoveries for vancomycin. Perfusate flow rates and drug concentrations affected the relative recovery when a combination of vancomycin and tobramycin was tested. Low perfusate flow rates were associated with higher recovery rates. For combination antibiotic measurement which includes agents that are highly protein bound, in vitro studies performed prior to in vivo studies may ensure the reliable measurement of unbound concentrations. Xi'an Jiaotong University 2018-12 2018-07-06 /pmc/articles/PMC6308031/ /pubmed/30595948 http://dx.doi.org/10.1016/j.jpha.2018.07.003 Text en © 2018 Xi'an Jiaotong University. Production and hosting by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Dhanani, Jayesh A.
Parker, Suzanne L.
Lipman, Jeffrey
Wallis, Steven C.
Cohen, Jeremy
Fraser, John
Barnett, Adrian
Chew, Michelle
Roberts, Jason A.
Recovery rates of combination antibiotic therapy using in vitro microdialysis simulating in vivo conditions
title Recovery rates of combination antibiotic therapy using in vitro microdialysis simulating in vivo conditions
title_full Recovery rates of combination antibiotic therapy using in vitro microdialysis simulating in vivo conditions
title_fullStr Recovery rates of combination antibiotic therapy using in vitro microdialysis simulating in vivo conditions
title_full_unstemmed Recovery rates of combination antibiotic therapy using in vitro microdialysis simulating in vivo conditions
title_short Recovery rates of combination antibiotic therapy using in vitro microdialysis simulating in vivo conditions
title_sort recovery rates of combination antibiotic therapy using in vitro microdialysis simulating in vivo conditions
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6308031/
https://www.ncbi.nlm.nih.gov/pubmed/30595948
http://dx.doi.org/10.1016/j.jpha.2018.07.003
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