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Normalization of Gene Expression by Quantitative RT-PCR in Human Cell Line: comparison of 12 Endogenous Reference Genes
BACKGROUND: Polymerase Chain Reaction (PCR) has become an important diagnostic and research tool of modern molecular biology globally. Real-time PCR allows for rapid and reliable quantification of mRNA transcription. Reference genes are used as internal reaction control to normalise mRNA levels betw...
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Research and Publications Office of Jimma University
2018
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6308757/ https://www.ncbi.nlm.nih.gov/pubmed/30607091 http://dx.doi.org/10.4314/ejhs.v28i6.9 |