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Effect of Conventional and Microwave Tissue Processing Technique on DNA Integrity: A Comparative Molecular Analysis

BACKGROUND: Methods of diagnostic molecular biology are routinely applied on formalin-fixed, paraffin-embedded tissues processed via conventional method. Recently, there has been a growing interest to use microwave technology in histopathology laboratories to overcome the deficiencies of the convent...

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Autores principales: Dwivedi, Dhara, Kasetty, Sowmya, Tijare, Manisha S, Kallianpur, Shreenivas, Prabhakar, Nitin, Ragavendra, Raju T, Desai, Ami
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Research and Publications Office of Jimma University 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6308768/
https://www.ncbi.nlm.nih.gov/pubmed/30607077
http://dx.doi.org/10.4314/ejhs.v28i5.13
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author Dwivedi, Dhara
Kasetty, Sowmya
Tijare, Manisha S
Kallianpur, Shreenivas
Prabhakar, Nitin
Ragavendra, Raju T
Desai, Ami
author_facet Dwivedi, Dhara
Kasetty, Sowmya
Tijare, Manisha S
Kallianpur, Shreenivas
Prabhakar, Nitin
Ragavendra, Raju T
Desai, Ami
author_sort Dwivedi, Dhara
collection PubMed
description BACKGROUND: Methods of diagnostic molecular biology are routinely applied on formalin-fixed, paraffin-embedded tissues processed via conventional method. Recently, there has been a growing interest to use microwave technology in histopathology laboratories to overcome the deficiencies of the conventional processing method. Thefore, this study was aimed to compare and analyze the quality and quantity of DNA obtained from tissues processed by conventional and microwave tissue processing techniques and to further ascertain the applicability of the latter for PCR (polymerase chain reaction based research). METHODS: Thirty fresh tissues of oral squamous cell carcinoma (OSCC) were included, and each sample was cut into two equivalent halves. One tissue half was processed by conventional manual method whereas the other half was processed using a domestic microwave oven. DNA was obtained from all the tissues which were then subjected to Polymerase chain reaction (PCR) to evaluate GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) gene expression. RESULTS: The results revealed better DNA yield from microwave processed tissue while the quality of the DNA was alike from both the techniques. CONCLUSION: On the basis of the results obtained, it can be concluded that DNA produced by microwave processed tissues was similar to that obtained by conventional processing technique in terms of quantity and quality. Thus, microwave processed tissue samples can be successfully used for further molecular studies and researches.
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spelling pubmed-63087682019-01-03 Effect of Conventional and Microwave Tissue Processing Technique on DNA Integrity: A Comparative Molecular Analysis Dwivedi, Dhara Kasetty, Sowmya Tijare, Manisha S Kallianpur, Shreenivas Prabhakar, Nitin Ragavendra, Raju T Desai, Ami Ethiop J Health Sci Original Article BACKGROUND: Methods of diagnostic molecular biology are routinely applied on formalin-fixed, paraffin-embedded tissues processed via conventional method. Recently, there has been a growing interest to use microwave technology in histopathology laboratories to overcome the deficiencies of the conventional processing method. Thefore, this study was aimed to compare and analyze the quality and quantity of DNA obtained from tissues processed by conventional and microwave tissue processing techniques and to further ascertain the applicability of the latter for PCR (polymerase chain reaction based research). METHODS: Thirty fresh tissues of oral squamous cell carcinoma (OSCC) were included, and each sample was cut into two equivalent halves. One tissue half was processed by conventional manual method whereas the other half was processed using a domestic microwave oven. DNA was obtained from all the tissues which were then subjected to Polymerase chain reaction (PCR) to evaluate GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) gene expression. RESULTS: The results revealed better DNA yield from microwave processed tissue while the quality of the DNA was alike from both the techniques. CONCLUSION: On the basis of the results obtained, it can be concluded that DNA produced by microwave processed tissues was similar to that obtained by conventional processing technique in terms of quantity and quality. Thus, microwave processed tissue samples can be successfully used for further molecular studies and researches. Research and Publications Office of Jimma University 2018-09 /pmc/articles/PMC6308768/ /pubmed/30607077 http://dx.doi.org/10.4314/ejhs.v28i5.13 Text en © 2018 Dhara D., et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Original Article
Dwivedi, Dhara
Kasetty, Sowmya
Tijare, Manisha S
Kallianpur, Shreenivas
Prabhakar, Nitin
Ragavendra, Raju T
Desai, Ami
Effect of Conventional and Microwave Tissue Processing Technique on DNA Integrity: A Comparative Molecular Analysis
title Effect of Conventional and Microwave Tissue Processing Technique on DNA Integrity: A Comparative Molecular Analysis
title_full Effect of Conventional and Microwave Tissue Processing Technique on DNA Integrity: A Comparative Molecular Analysis
title_fullStr Effect of Conventional and Microwave Tissue Processing Technique on DNA Integrity: A Comparative Molecular Analysis
title_full_unstemmed Effect of Conventional and Microwave Tissue Processing Technique on DNA Integrity: A Comparative Molecular Analysis
title_short Effect of Conventional and Microwave Tissue Processing Technique on DNA Integrity: A Comparative Molecular Analysis
title_sort effect of conventional and microwave tissue processing technique on dna integrity: a comparative molecular analysis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6308768/
https://www.ncbi.nlm.nih.gov/pubmed/30607077
http://dx.doi.org/10.4314/ejhs.v28i5.13
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