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Zingiber Officinale Roscoe and Echinops Kebericho Mesfin Showed Antiplasmodial Activities against Plasmodium Berghei in a Dose-dependent Manner in Ethiopia
BACKGROUND: The emergence and spread of Plasmodium falciparum resistance to antimalarial drugs necessitated the search for new drugs from natural products. Zingiber officinal Roscoe and Echinops Kebericho Mesfin are traditional herbal medicines widely used for the treatment of malaria in Ethiopia. T...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Research and Publications Office of Jimma University
2018
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6308778/ https://www.ncbi.nlm.nih.gov/pubmed/30607081 http://dx.doi.org/10.4314/ejhs.v28i5.17 |
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author | Biruksew, Abdissa Zeynudin, Ahmed Alemu, Yonas Golassa, Lemu Yohannes, Moti Debella, Asfaw Urge, Geme De Spiegeleer, Bart Suleman, Sultan |
author_facet | Biruksew, Abdissa Zeynudin, Ahmed Alemu, Yonas Golassa, Lemu Yohannes, Moti Debella, Asfaw Urge, Geme De Spiegeleer, Bart Suleman, Sultan |
author_sort | Biruksew, Abdissa |
collection | PubMed |
description | BACKGROUND: The emergence and spread of Plasmodium falciparum resistance to antimalarial drugs necessitated the search for new drugs from natural products. Zingiber officinal Roscoe and Echinops Kebericho Mesfin are traditional herbal medicines widely used for the treatment of malaria in Ethiopia. The aim of the study was to assess the toxicity profile and in vivo antiplasmodial activities of 70% methanol crude extracts of both plant materials against Plasmodium berghei. METHODS: Healthy male Swiss Albino mice of age 4–5 weeks and weight 25–36 g were infected by P. berghei. The extracts were administered orally at doses 5000, 2500 and 1250 mg/kg for acute toxicity of E. kebericho Mesfin. Graded doses at 1000, 500 and 250 mg/kg used for four days suppressive studies. Parasitemia, body weight, packed cell volume (PCV) and survival time were determined. SPSS Version 20 was used for the analysis of data of parasitemia, body weight, PCV, and survival times. Statistical significance was determined by one-way ANOVA. Independent t-test was used to compare results. Results were presented as a mean ± standard error of the mean (M ± SEM). All data were analyzed at a 95% confidence interval (α= 0.05). RESULTS: At the dose of 5000 mg/kg, E. kebericho Mesfin showed no toxic effects. The LD50 of extract could go beyond the dose used. In vivo antiplasmodial activity of extracts showed excellent chemo suppression at 500 and 1000 mg/kg in a dose dependent manner compared with the negative control. The chemo suppressions of the 1000 mg/kg of both plant extracts were 49.53 ± 1.90% and 32.83 ± 1.03%, respectively. The survival times of P. berghei infected mice were also a dose dependent manner while failed to prevent weight loss. CONCLUSION: The extracts of both medicinal plants showed antiplasmodial activities against P. berghei. It confirmed the literature findings and their traditional uses. |
format | Online Article Text |
id | pubmed-6308778 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Research and Publications Office of Jimma University |
record_format | MEDLINE/PubMed |
spelling | pubmed-63087782019-01-03 Zingiber Officinale Roscoe and Echinops Kebericho Mesfin Showed Antiplasmodial Activities against Plasmodium Berghei in a Dose-dependent Manner in Ethiopia Biruksew, Abdissa Zeynudin, Ahmed Alemu, Yonas Golassa, Lemu Yohannes, Moti Debella, Asfaw Urge, Geme De Spiegeleer, Bart Suleman, Sultan Ethiop J Health Sci Original Article BACKGROUND: The emergence and spread of Plasmodium falciparum resistance to antimalarial drugs necessitated the search for new drugs from natural products. Zingiber officinal Roscoe and Echinops Kebericho Mesfin are traditional herbal medicines widely used for the treatment of malaria in Ethiopia. The aim of the study was to assess the toxicity profile and in vivo antiplasmodial activities of 70% methanol crude extracts of both plant materials against Plasmodium berghei. METHODS: Healthy male Swiss Albino mice of age 4–5 weeks and weight 25–36 g were infected by P. berghei. The extracts were administered orally at doses 5000, 2500 and 1250 mg/kg for acute toxicity of E. kebericho Mesfin. Graded doses at 1000, 500 and 250 mg/kg used for four days suppressive studies. Parasitemia, body weight, packed cell volume (PCV) and survival time were determined. SPSS Version 20 was used for the analysis of data of parasitemia, body weight, PCV, and survival times. Statistical significance was determined by one-way ANOVA. Independent t-test was used to compare results. Results were presented as a mean ± standard error of the mean (M ± SEM). All data were analyzed at a 95% confidence interval (α= 0.05). RESULTS: At the dose of 5000 mg/kg, E. kebericho Mesfin showed no toxic effects. The LD50 of extract could go beyond the dose used. In vivo antiplasmodial activity of extracts showed excellent chemo suppression at 500 and 1000 mg/kg in a dose dependent manner compared with the negative control. The chemo suppressions of the 1000 mg/kg of both plant extracts were 49.53 ± 1.90% and 32.83 ± 1.03%, respectively. The survival times of P. berghei infected mice were also a dose dependent manner while failed to prevent weight loss. CONCLUSION: The extracts of both medicinal plants showed antiplasmodial activities against P. berghei. It confirmed the literature findings and their traditional uses. Research and Publications Office of Jimma University 2018-09 /pmc/articles/PMC6308778/ /pubmed/30607081 http://dx.doi.org/10.4314/ejhs.v28i5.17 Text en © 2018 Abdissa Biruksew, et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Original Article Biruksew, Abdissa Zeynudin, Ahmed Alemu, Yonas Golassa, Lemu Yohannes, Moti Debella, Asfaw Urge, Geme De Spiegeleer, Bart Suleman, Sultan Zingiber Officinale Roscoe and Echinops Kebericho Mesfin Showed Antiplasmodial Activities against Plasmodium Berghei in a Dose-dependent Manner in Ethiopia |
title | Zingiber Officinale Roscoe and Echinops Kebericho Mesfin Showed Antiplasmodial Activities against Plasmodium Berghei in a Dose-dependent Manner in Ethiopia |
title_full | Zingiber Officinale Roscoe and Echinops Kebericho Mesfin Showed Antiplasmodial Activities against Plasmodium Berghei in a Dose-dependent Manner in Ethiopia |
title_fullStr | Zingiber Officinale Roscoe and Echinops Kebericho Mesfin Showed Antiplasmodial Activities against Plasmodium Berghei in a Dose-dependent Manner in Ethiopia |
title_full_unstemmed | Zingiber Officinale Roscoe and Echinops Kebericho Mesfin Showed Antiplasmodial Activities against Plasmodium Berghei in a Dose-dependent Manner in Ethiopia |
title_short | Zingiber Officinale Roscoe and Echinops Kebericho Mesfin Showed Antiplasmodial Activities against Plasmodium Berghei in a Dose-dependent Manner in Ethiopia |
title_sort | zingiber officinale roscoe and echinops kebericho mesfin showed antiplasmodial activities against plasmodium berghei in a dose-dependent manner in ethiopia |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6308778/ https://www.ncbi.nlm.nih.gov/pubmed/30607081 http://dx.doi.org/10.4314/ejhs.v28i5.17 |
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