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A Ketoconazole Susceptibility Test for Malassezia pachydermatis Using Modified Leeming–Notman Agar

The aim of this study was to establish a ketoconazole susceptibility test for Malassezia pachydermatis using modified Leeming–Notman agar (mLNA). The susceptibilities of 33 M. pachydermatis isolates obtained by modified CLSI M27-A3 method were compared with the results by disk diffusion method, whic...

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Autores principales: Hsieh, Bo-Young, Chao, Wei-Hsun, Xue, Yi-Jing, Lai, Jyh-Mirn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6308931/
https://www.ncbi.nlm.nih.gov/pubmed/30453525
http://dx.doi.org/10.3390/jof4040126
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author Hsieh, Bo-Young
Chao, Wei-Hsun
Xue, Yi-Jing
Lai, Jyh-Mirn
author_facet Hsieh, Bo-Young
Chao, Wei-Hsun
Xue, Yi-Jing
Lai, Jyh-Mirn
author_sort Hsieh, Bo-Young
collection PubMed
description The aim of this study was to establish a ketoconazole susceptibility test for Malassezia pachydermatis using modified Leeming–Notman agar (mLNA). The susceptibilities of 33 M. pachydermatis isolates obtained by modified CLSI M27-A3 method were compared with the results by disk diffusion method, which used different concentrations of ketoconazole on 6 mm diameter paper disks. Results showed that 93.9% (31/33) of the minimum inhibitory concentration (MIC) values obtained from both methods were similar (consistent with two methods within 2 dilutions). M. pachydermatis BCRC 21676 and Candida parapsilosis ATCC 22019 were used to verify the results obtained from the disk diffusion and modified CLSI M27-A3 tests, and they were found to be consistent. Therefore, the current study concludes that this new novel test—using different concentrations of reagents on cartridge disks to detect MIC values against ketoconazole—can be a cost-effective, time-efficient, and less technically demanding alternative to existing methods.
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spelling pubmed-63089312019-06-17 A Ketoconazole Susceptibility Test for Malassezia pachydermatis Using Modified Leeming–Notman Agar Hsieh, Bo-Young Chao, Wei-Hsun Xue, Yi-Jing Lai, Jyh-Mirn J Fungi (Basel) Article The aim of this study was to establish a ketoconazole susceptibility test for Malassezia pachydermatis using modified Leeming–Notman agar (mLNA). The susceptibilities of 33 M. pachydermatis isolates obtained by modified CLSI M27-A3 method were compared with the results by disk diffusion method, which used different concentrations of ketoconazole on 6 mm diameter paper disks. Results showed that 93.9% (31/33) of the minimum inhibitory concentration (MIC) values obtained from both methods were similar (consistent with two methods within 2 dilutions). M. pachydermatis BCRC 21676 and Candida parapsilosis ATCC 22019 were used to verify the results obtained from the disk diffusion and modified CLSI M27-A3 tests, and they were found to be consistent. Therefore, the current study concludes that this new novel test—using different concentrations of reagents on cartridge disks to detect MIC values against ketoconazole—can be a cost-effective, time-efficient, and less technically demanding alternative to existing methods. MDPI 2018-11-16 /pmc/articles/PMC6308931/ /pubmed/30453525 http://dx.doi.org/10.3390/jof4040126 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hsieh, Bo-Young
Chao, Wei-Hsun
Xue, Yi-Jing
Lai, Jyh-Mirn
A Ketoconazole Susceptibility Test for Malassezia pachydermatis Using Modified Leeming–Notman Agar
title A Ketoconazole Susceptibility Test for Malassezia pachydermatis Using Modified Leeming–Notman Agar
title_full A Ketoconazole Susceptibility Test for Malassezia pachydermatis Using Modified Leeming–Notman Agar
title_fullStr A Ketoconazole Susceptibility Test for Malassezia pachydermatis Using Modified Leeming–Notman Agar
title_full_unstemmed A Ketoconazole Susceptibility Test for Malassezia pachydermatis Using Modified Leeming–Notman Agar
title_short A Ketoconazole Susceptibility Test for Malassezia pachydermatis Using Modified Leeming–Notman Agar
title_sort ketoconazole susceptibility test for malassezia pachydermatis using modified leeming–notman agar
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6308931/
https://www.ncbi.nlm.nih.gov/pubmed/30453525
http://dx.doi.org/10.3390/jof4040126
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