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The single mitochondrion of the kinetoplastid parasite Crithidia fasciculata is a dynamic network

Mitochondria are central organelles in cellular metabolism. Their structure is highly dynamic, allowing them to adapt to different energy requirements, to be partitioned during cell division, and to maintain functionality. Mitochondrial dynamics, including membrane fusion and fission reactions, are...

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Autores principales: DiMaio, John, Ruthel, Gordon, Cannon, Joshua J., Malfara, Madeline F., Povelones, Megan L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6310254/
https://www.ncbi.nlm.nih.gov/pubmed/30592713
http://dx.doi.org/10.1371/journal.pone.0202711
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author DiMaio, John
Ruthel, Gordon
Cannon, Joshua J.
Malfara, Madeline F.
Povelones, Megan L.
author_facet DiMaio, John
Ruthel, Gordon
Cannon, Joshua J.
Malfara, Madeline F.
Povelones, Megan L.
author_sort DiMaio, John
collection PubMed
description Mitochondria are central organelles in cellular metabolism. Their structure is highly dynamic, allowing them to adapt to different energy requirements, to be partitioned during cell division, and to maintain functionality. Mitochondrial dynamics, including membrane fusion and fission reactions, are well studied in yeast and mammals but it is not known if these processes are conserved throughout eukaryotic evolution. Kinetoplastid parasites are some of the earliest-diverging eukaryotes to retain a mitochondrion. Each cell has only a single mitochondrial organelle, making them an interesting model for the role of dynamics in controlling mitochondrial architecture. We have investigated the mitochondrial division cycle in the kinetoplastid Crithidia fasciculata. The majority of mitochondrial biogenesis occurs during the G1 phase of the cell cycle, and the mitochondrion is divided symmetrically in a process coincident with cytokinesis. Live cell imaging revealed that the mitochondrion is highly dynamic, with frequent changes in the topology of the branched network. These remodeling reactions include tubule fission, fusion, and sliding, as well as new tubule formation. We hypothesize that the function of this dynamic remodeling is to homogenize mitochondrial contents and to facilitate rapid transport of mitochondria-encoded gene products from the area containing the mitochondrial nucleoid to other parts of the organelle.
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spelling pubmed-63102542019-01-08 The single mitochondrion of the kinetoplastid parasite Crithidia fasciculata is a dynamic network DiMaio, John Ruthel, Gordon Cannon, Joshua J. Malfara, Madeline F. Povelones, Megan L. PLoS One Research Article Mitochondria are central organelles in cellular metabolism. Their structure is highly dynamic, allowing them to adapt to different energy requirements, to be partitioned during cell division, and to maintain functionality. Mitochondrial dynamics, including membrane fusion and fission reactions, are well studied in yeast and mammals but it is not known if these processes are conserved throughout eukaryotic evolution. Kinetoplastid parasites are some of the earliest-diverging eukaryotes to retain a mitochondrion. Each cell has only a single mitochondrial organelle, making them an interesting model for the role of dynamics in controlling mitochondrial architecture. We have investigated the mitochondrial division cycle in the kinetoplastid Crithidia fasciculata. The majority of mitochondrial biogenesis occurs during the G1 phase of the cell cycle, and the mitochondrion is divided symmetrically in a process coincident with cytokinesis. Live cell imaging revealed that the mitochondrion is highly dynamic, with frequent changes in the topology of the branched network. These remodeling reactions include tubule fission, fusion, and sliding, as well as new tubule formation. We hypothesize that the function of this dynamic remodeling is to homogenize mitochondrial contents and to facilitate rapid transport of mitochondria-encoded gene products from the area containing the mitochondrial nucleoid to other parts of the organelle. Public Library of Science 2018-12-28 /pmc/articles/PMC6310254/ /pubmed/30592713 http://dx.doi.org/10.1371/journal.pone.0202711 Text en © 2018 DiMaio et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
DiMaio, John
Ruthel, Gordon
Cannon, Joshua J.
Malfara, Madeline F.
Povelones, Megan L.
The single mitochondrion of the kinetoplastid parasite Crithidia fasciculata is a dynamic network
title The single mitochondrion of the kinetoplastid parasite Crithidia fasciculata is a dynamic network
title_full The single mitochondrion of the kinetoplastid parasite Crithidia fasciculata is a dynamic network
title_fullStr The single mitochondrion of the kinetoplastid parasite Crithidia fasciculata is a dynamic network
title_full_unstemmed The single mitochondrion of the kinetoplastid parasite Crithidia fasciculata is a dynamic network
title_short The single mitochondrion of the kinetoplastid parasite Crithidia fasciculata is a dynamic network
title_sort single mitochondrion of the kinetoplastid parasite crithidia fasciculata is a dynamic network
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6310254/
https://www.ncbi.nlm.nih.gov/pubmed/30592713
http://dx.doi.org/10.1371/journal.pone.0202711
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