Cargando…

A comparison study of five different methods to measure carotenoids in biofortified yellow cassava (Manihot esculenta)

The most commonly used method for measuring carotenoid concentration is high-performance liquid chromatography (HPLC). Nevertheless, easier, quicker, and less costly proxy methods exist. We aimed to determine the diagnostic performance of several proxy methods: the spectrophotometer, iCheck Carotene...

Descripción completa

Detalles Bibliográficos
Autores principales: Jaramillo, Angélica M., Londoño, Luis Fernando, Orozco, Juan Camilo, Patiño, Gelver, Belalcazar, John, Davrieux, Fabrice, Talsma, Elise F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6310354/
https://www.ncbi.nlm.nih.gov/pubmed/30592746
http://dx.doi.org/10.1371/journal.pone.0209702
_version_ 1783383416646926336
author Jaramillo, Angélica M.
Londoño, Luis Fernando
Orozco, Juan Camilo
Patiño, Gelver
Belalcazar, John
Davrieux, Fabrice
Talsma, Elise F.
author_facet Jaramillo, Angélica M.
Londoño, Luis Fernando
Orozco, Juan Camilo
Patiño, Gelver
Belalcazar, John
Davrieux, Fabrice
Talsma, Elise F.
author_sort Jaramillo, Angélica M.
collection PubMed
description The most commonly used method for measuring carotenoid concentration is high-performance liquid chromatography (HPLC). Nevertheless, easier, quicker, and less costly proxy methods exist. We aimed to determine the diagnostic performance of several proxy methods: the spectrophotometer, iCheck Carotene, and near-infrared spectroscopy using both a desktop (dNIRS) and a portable (pNIRS) device for the measurement of total carotenoid concentration (TCC) and all-trans-β-carotene concentration (trans-BC) in 30 fresh cassava (Manihot esculenta Crantz) storage roots in comparison with HPLC. The spectrophotometer presented the highest predictability for TCC, followed by iCheck, dNIRS, and pNIRS. The dNIRS showed the highest predictability and agreement for trans-BC. The pNIRS showed the poorest repeatability and greatest underestimations compared with HPLC. The agreement between all methods was lower for higher carotenoid concentration, with the exception of the spectrophotometer. According to our results, and for screening purposes, the measurement of carotenoids in fresh cassava roots can be carried out by spectrophotometer, iCheck Carotene and NIRS methods depending on the availability of equipment.
format Online
Article
Text
id pubmed-6310354
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-63103542019-01-08 A comparison study of five different methods to measure carotenoids in biofortified yellow cassava (Manihot esculenta) Jaramillo, Angélica M. Londoño, Luis Fernando Orozco, Juan Camilo Patiño, Gelver Belalcazar, John Davrieux, Fabrice Talsma, Elise F. PLoS One Research Article The most commonly used method for measuring carotenoid concentration is high-performance liquid chromatography (HPLC). Nevertheless, easier, quicker, and less costly proxy methods exist. We aimed to determine the diagnostic performance of several proxy methods: the spectrophotometer, iCheck Carotene, and near-infrared spectroscopy using both a desktop (dNIRS) and a portable (pNIRS) device for the measurement of total carotenoid concentration (TCC) and all-trans-β-carotene concentration (trans-BC) in 30 fresh cassava (Manihot esculenta Crantz) storage roots in comparison with HPLC. The spectrophotometer presented the highest predictability for TCC, followed by iCheck, dNIRS, and pNIRS. The dNIRS showed the highest predictability and agreement for trans-BC. The pNIRS showed the poorest repeatability and greatest underestimations compared with HPLC. The agreement between all methods was lower for higher carotenoid concentration, with the exception of the spectrophotometer. According to our results, and for screening purposes, the measurement of carotenoids in fresh cassava roots can be carried out by spectrophotometer, iCheck Carotene and NIRS methods depending on the availability of equipment. Public Library of Science 2018-12-28 /pmc/articles/PMC6310354/ /pubmed/30592746 http://dx.doi.org/10.1371/journal.pone.0209702 Text en © 2018 Jaramillo et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Jaramillo, Angélica M.
Londoño, Luis Fernando
Orozco, Juan Camilo
Patiño, Gelver
Belalcazar, John
Davrieux, Fabrice
Talsma, Elise F.
A comparison study of five different methods to measure carotenoids in biofortified yellow cassava (Manihot esculenta)
title A comparison study of five different methods to measure carotenoids in biofortified yellow cassava (Manihot esculenta)
title_full A comparison study of five different methods to measure carotenoids in biofortified yellow cassava (Manihot esculenta)
title_fullStr A comparison study of five different methods to measure carotenoids in biofortified yellow cassava (Manihot esculenta)
title_full_unstemmed A comparison study of five different methods to measure carotenoids in biofortified yellow cassava (Manihot esculenta)
title_short A comparison study of five different methods to measure carotenoids in biofortified yellow cassava (Manihot esculenta)
title_sort comparison study of five different methods to measure carotenoids in biofortified yellow cassava (manihot esculenta)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6310354/
https://www.ncbi.nlm.nih.gov/pubmed/30592746
http://dx.doi.org/10.1371/journal.pone.0209702
work_keys_str_mv AT jaramilloangelicam acomparisonstudyoffivedifferentmethodstomeasurecarotenoidsinbiofortifiedyellowcassavamanihotesculenta
AT londonoluisfernando acomparisonstudyoffivedifferentmethodstomeasurecarotenoidsinbiofortifiedyellowcassavamanihotesculenta
AT orozcojuancamilo acomparisonstudyoffivedifferentmethodstomeasurecarotenoidsinbiofortifiedyellowcassavamanihotesculenta
AT patinogelver acomparisonstudyoffivedifferentmethodstomeasurecarotenoidsinbiofortifiedyellowcassavamanihotesculenta
AT belalcazarjohn acomparisonstudyoffivedifferentmethodstomeasurecarotenoidsinbiofortifiedyellowcassavamanihotesculenta
AT davrieuxfabrice acomparisonstudyoffivedifferentmethodstomeasurecarotenoidsinbiofortifiedyellowcassavamanihotesculenta
AT talsmaelisef acomparisonstudyoffivedifferentmethodstomeasurecarotenoidsinbiofortifiedyellowcassavamanihotesculenta
AT jaramilloangelicam comparisonstudyoffivedifferentmethodstomeasurecarotenoidsinbiofortifiedyellowcassavamanihotesculenta
AT londonoluisfernando comparisonstudyoffivedifferentmethodstomeasurecarotenoidsinbiofortifiedyellowcassavamanihotesculenta
AT orozcojuancamilo comparisonstudyoffivedifferentmethodstomeasurecarotenoidsinbiofortifiedyellowcassavamanihotesculenta
AT patinogelver comparisonstudyoffivedifferentmethodstomeasurecarotenoidsinbiofortifiedyellowcassavamanihotesculenta
AT belalcazarjohn comparisonstudyoffivedifferentmethodstomeasurecarotenoidsinbiofortifiedyellowcassavamanihotesculenta
AT davrieuxfabrice comparisonstudyoffivedifferentmethodstomeasurecarotenoidsinbiofortifiedyellowcassavamanihotesculenta
AT talsmaelisef comparisonstudyoffivedifferentmethodstomeasurecarotenoidsinbiofortifiedyellowcassavamanihotesculenta