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A comparison study of five different methods to measure carotenoids in biofortified yellow cassava (Manihot esculenta)
The most commonly used method for measuring carotenoid concentration is high-performance liquid chromatography (HPLC). Nevertheless, easier, quicker, and less costly proxy methods exist. We aimed to determine the diagnostic performance of several proxy methods: the spectrophotometer, iCheck Carotene...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6310354/ https://www.ncbi.nlm.nih.gov/pubmed/30592746 http://dx.doi.org/10.1371/journal.pone.0209702 |
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author | Jaramillo, Angélica M. Londoño, Luis Fernando Orozco, Juan Camilo Patiño, Gelver Belalcazar, John Davrieux, Fabrice Talsma, Elise F. |
author_facet | Jaramillo, Angélica M. Londoño, Luis Fernando Orozco, Juan Camilo Patiño, Gelver Belalcazar, John Davrieux, Fabrice Talsma, Elise F. |
author_sort | Jaramillo, Angélica M. |
collection | PubMed |
description | The most commonly used method for measuring carotenoid concentration is high-performance liquid chromatography (HPLC). Nevertheless, easier, quicker, and less costly proxy methods exist. We aimed to determine the diagnostic performance of several proxy methods: the spectrophotometer, iCheck Carotene, and near-infrared spectroscopy using both a desktop (dNIRS) and a portable (pNIRS) device for the measurement of total carotenoid concentration (TCC) and all-trans-β-carotene concentration (trans-BC) in 30 fresh cassava (Manihot esculenta Crantz) storage roots in comparison with HPLC. The spectrophotometer presented the highest predictability for TCC, followed by iCheck, dNIRS, and pNIRS. The dNIRS showed the highest predictability and agreement for trans-BC. The pNIRS showed the poorest repeatability and greatest underestimations compared with HPLC. The agreement between all methods was lower for higher carotenoid concentration, with the exception of the spectrophotometer. According to our results, and for screening purposes, the measurement of carotenoids in fresh cassava roots can be carried out by spectrophotometer, iCheck Carotene and NIRS methods depending on the availability of equipment. |
format | Online Article Text |
id | pubmed-6310354 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-63103542019-01-08 A comparison study of five different methods to measure carotenoids in biofortified yellow cassava (Manihot esculenta) Jaramillo, Angélica M. Londoño, Luis Fernando Orozco, Juan Camilo Patiño, Gelver Belalcazar, John Davrieux, Fabrice Talsma, Elise F. PLoS One Research Article The most commonly used method for measuring carotenoid concentration is high-performance liquid chromatography (HPLC). Nevertheless, easier, quicker, and less costly proxy methods exist. We aimed to determine the diagnostic performance of several proxy methods: the spectrophotometer, iCheck Carotene, and near-infrared spectroscopy using both a desktop (dNIRS) and a portable (pNIRS) device for the measurement of total carotenoid concentration (TCC) and all-trans-β-carotene concentration (trans-BC) in 30 fresh cassava (Manihot esculenta Crantz) storage roots in comparison with HPLC. The spectrophotometer presented the highest predictability for TCC, followed by iCheck, dNIRS, and pNIRS. The dNIRS showed the highest predictability and agreement for trans-BC. The pNIRS showed the poorest repeatability and greatest underestimations compared with HPLC. The agreement between all methods was lower for higher carotenoid concentration, with the exception of the spectrophotometer. According to our results, and for screening purposes, the measurement of carotenoids in fresh cassava roots can be carried out by spectrophotometer, iCheck Carotene and NIRS methods depending on the availability of equipment. Public Library of Science 2018-12-28 /pmc/articles/PMC6310354/ /pubmed/30592746 http://dx.doi.org/10.1371/journal.pone.0209702 Text en © 2018 Jaramillo et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Jaramillo, Angélica M. Londoño, Luis Fernando Orozco, Juan Camilo Patiño, Gelver Belalcazar, John Davrieux, Fabrice Talsma, Elise F. A comparison study of five different methods to measure carotenoids in biofortified yellow cassava (Manihot esculenta) |
title | A comparison study of five different methods to measure carotenoids in biofortified yellow cassava (Manihot esculenta) |
title_full | A comparison study of five different methods to measure carotenoids in biofortified yellow cassava (Manihot esculenta) |
title_fullStr | A comparison study of five different methods to measure carotenoids in biofortified yellow cassava (Manihot esculenta) |
title_full_unstemmed | A comparison study of five different methods to measure carotenoids in biofortified yellow cassava (Manihot esculenta) |
title_short | A comparison study of five different methods to measure carotenoids in biofortified yellow cassava (Manihot esculenta) |
title_sort | comparison study of five different methods to measure carotenoids in biofortified yellow cassava (manihot esculenta) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6310354/ https://www.ncbi.nlm.nih.gov/pubmed/30592746 http://dx.doi.org/10.1371/journal.pone.0209702 |
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