Cargando…

Analysis of PTEN expression and promoter methylation in Uyghur patients with mild type 2 diabetes mellitus

Phosphatase and tension homolog deleted on chromosome 10 (PTEN) was considered as a promising target in type 2 diabetes mellitus (T2DM) because of its negative effects on insulin resistance. Alteration in DNA methylation is thought to play a role in the pathogenesis of T2DM. The aim of the present s...

Descripción completa

Detalles Bibliográficos
Autores principales: Yin, Liang, Cai, Wei-Juan, Chang, Xiang-Yun, Li, Jun, Zhu, Ling-Yun, Su, Xiang-Hui, Yu, Xue-Feng, Sun, Kan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer Health 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6310531/
https://www.ncbi.nlm.nih.gov/pubmed/30544451
http://dx.doi.org/10.1097/MD.0000000000013513
_version_ 1783383454070603776
author Yin, Liang
Cai, Wei-Juan
Chang, Xiang-Yun
Li, Jun
Zhu, Ling-Yun
Su, Xiang-Hui
Yu, Xue-Feng
Sun, Kan
author_facet Yin, Liang
Cai, Wei-Juan
Chang, Xiang-Yun
Li, Jun
Zhu, Ling-Yun
Su, Xiang-Hui
Yu, Xue-Feng
Sun, Kan
author_sort Yin, Liang
collection PubMed
description Phosphatase and tension homolog deleted on chromosome 10 (PTEN) was considered as a promising target in type 2 diabetes mellitus (T2DM) because of its negative effects on insulin resistance. Alteration in DNA methylation is thought to play a role in the pathogenesis of T2DM. The aim of the present study was to quantitatively evaluate the promoter methylation of PTEN in Uyghur patients with mild T2DM. We evaluated methylation levels in 21 CpG sites from −2515 bp to −2186 bp relative to the translation initiation site in 55 cases of T2DM and 50 cases of normal glucose tolerance (NGT) using the MassARRAY spectrometry. In addition, PTEN mRNA and protein levels were measured by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) and western blotting to determine whether DNA methylation alterations were responsible for PTEN expression. Compared with NGT groups, the PTEN mRNA expression was significantly higher in Uyghur patients with mild T2DM groups. We also showed that PTEN protein expression was upregulated in Uyghur patients with mild T2DM groups, but the level of protein kinase B (AKT) was downregulated. PTEN methylation in T2DM patients was significantly lower than that in NGT groups. In addition, 2 CpG units demonstrated a significant difference between the NGT and Uyghur patients with mild T2DM groups. Furthermore, there was a negative association between promoter methylation and PTEN expression. Together, these findings suggest that epigenetic inactivation of PTEN plays an important role in Uyghur patients with mild T2DM. The aberrant methylation of CpG sites within the PTEN promoter may serve as a potential candidate biomarker for T2DM in the Uyghur population.
format Online
Article
Text
id pubmed-6310531
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Wolters Kluwer Health
record_format MEDLINE/PubMed
spelling pubmed-63105312019-01-14 Analysis of PTEN expression and promoter methylation in Uyghur patients with mild type 2 diabetes mellitus Yin, Liang Cai, Wei-Juan Chang, Xiang-Yun Li, Jun Zhu, Ling-Yun Su, Xiang-Hui Yu, Xue-Feng Sun, Kan Medicine (Baltimore) Research Article Phosphatase and tension homolog deleted on chromosome 10 (PTEN) was considered as a promising target in type 2 diabetes mellitus (T2DM) because of its negative effects on insulin resistance. Alteration in DNA methylation is thought to play a role in the pathogenesis of T2DM. The aim of the present study was to quantitatively evaluate the promoter methylation of PTEN in Uyghur patients with mild T2DM. We evaluated methylation levels in 21 CpG sites from −2515 bp to −2186 bp relative to the translation initiation site in 55 cases of T2DM and 50 cases of normal glucose tolerance (NGT) using the MassARRAY spectrometry. In addition, PTEN mRNA and protein levels were measured by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) and western blotting to determine whether DNA methylation alterations were responsible for PTEN expression. Compared with NGT groups, the PTEN mRNA expression was significantly higher in Uyghur patients with mild T2DM groups. We also showed that PTEN protein expression was upregulated in Uyghur patients with mild T2DM groups, but the level of protein kinase B (AKT) was downregulated. PTEN methylation in T2DM patients was significantly lower than that in NGT groups. In addition, 2 CpG units demonstrated a significant difference between the NGT and Uyghur patients with mild T2DM groups. Furthermore, there was a negative association between promoter methylation and PTEN expression. Together, these findings suggest that epigenetic inactivation of PTEN plays an important role in Uyghur patients with mild T2DM. The aberrant methylation of CpG sites within the PTEN promoter may serve as a potential candidate biomarker for T2DM in the Uyghur population. Wolters Kluwer Health 2018-12-10 /pmc/articles/PMC6310531/ /pubmed/30544451 http://dx.doi.org/10.1097/MD.0000000000013513 Text en Copyright © 2018 the Author(s). Published by Wolters Kluwer Health, Inc. http://creativecommons.org/licenses/by-nc-nd/4.0 This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. http://creativecommons.org/licenses/by-nc-nd/4.0
spellingShingle Research Article
Yin, Liang
Cai, Wei-Juan
Chang, Xiang-Yun
Li, Jun
Zhu, Ling-Yun
Su, Xiang-Hui
Yu, Xue-Feng
Sun, Kan
Analysis of PTEN expression and promoter methylation in Uyghur patients with mild type 2 diabetes mellitus
title Analysis of PTEN expression and promoter methylation in Uyghur patients with mild type 2 diabetes mellitus
title_full Analysis of PTEN expression and promoter methylation in Uyghur patients with mild type 2 diabetes mellitus
title_fullStr Analysis of PTEN expression and promoter methylation in Uyghur patients with mild type 2 diabetes mellitus
title_full_unstemmed Analysis of PTEN expression and promoter methylation in Uyghur patients with mild type 2 diabetes mellitus
title_short Analysis of PTEN expression and promoter methylation in Uyghur patients with mild type 2 diabetes mellitus
title_sort analysis of pten expression and promoter methylation in uyghur patients with mild type 2 diabetes mellitus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6310531/
https://www.ncbi.nlm.nih.gov/pubmed/30544451
http://dx.doi.org/10.1097/MD.0000000000013513
work_keys_str_mv AT yinliang analysisofptenexpressionandpromotermethylationinuyghurpatientswithmildtype2diabetesmellitus
AT caiweijuan analysisofptenexpressionandpromotermethylationinuyghurpatientswithmildtype2diabetesmellitus
AT changxiangyun analysisofptenexpressionandpromotermethylationinuyghurpatientswithmildtype2diabetesmellitus
AT lijun analysisofptenexpressionandpromotermethylationinuyghurpatientswithmildtype2diabetesmellitus
AT zhulingyun analysisofptenexpressionandpromotermethylationinuyghurpatientswithmildtype2diabetesmellitus
AT suxianghui analysisofptenexpressionandpromotermethylationinuyghurpatientswithmildtype2diabetesmellitus
AT yuxuefeng analysisofptenexpressionandpromotermethylationinuyghurpatientswithmildtype2diabetesmellitus
AT sunkan analysisofptenexpressionandpromotermethylationinuyghurpatientswithmildtype2diabetesmellitus