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Exploiting correlated molecular-dynamics networks to counteract enzyme activity–stability trade-off
The directed evolution of enzymes for improved activity or substrate specificity commonly leads to a trade-off in stability. We have identified an activity–stability trade-off and a loss in unfolding cooperativity for a variant (3M) of Escherichia coli transketolase (TK) engineered to accept aromati...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
National Academy of Sciences
2018
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6310800/ https://www.ncbi.nlm.nih.gov/pubmed/30530661 http://dx.doi.org/10.1073/pnas.1812204115 |