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Identification of Cytauxzoon felis antigens via protein microarray and assessment of expression library immunization against cytauxzoonosis

BACKGROUND: Cytauxzoonosis is a disease of felids in North America caused by the tick-transmitted apicomplexan parasite Cytauxzoon felis. Cytauxzoonosis is particularly virulent for domestic cats, but no vaccine currently exists. The parasite cannot be cultivated in vitro, presenting a significant l...

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Autores principales: Schreeg, Megan E., Marr, Henry S., Tarigo, Jaime L., Sherrill, Meredith K., Outi, Hilton K., Scholl, Elizabeth H., Bird, David M., Vigil, Adam, Hung, Chris, Nakajima, Rie, Liang, Li, Trieu, Angela, Doolan, Denise L., Thomas, Jennifer E., Levy, Michael G., Reichard, Mason V., Felgner, Philip L., Cohn, Leah A., Birkenheuer, Adam J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6310948/
https://www.ncbi.nlm.nih.gov/pubmed/30618510
http://dx.doi.org/10.1186/s12014-018-9218-9
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author Schreeg, Megan E.
Marr, Henry S.
Tarigo, Jaime L.
Sherrill, Meredith K.
Outi, Hilton K.
Scholl, Elizabeth H.
Bird, David M.
Vigil, Adam
Hung, Chris
Nakajima, Rie
Liang, Li
Trieu, Angela
Doolan, Denise L.
Thomas, Jennifer E.
Levy, Michael G.
Reichard, Mason V.
Felgner, Philip L.
Cohn, Leah A.
Birkenheuer, Adam J.
author_facet Schreeg, Megan E.
Marr, Henry S.
Tarigo, Jaime L.
Sherrill, Meredith K.
Outi, Hilton K.
Scholl, Elizabeth H.
Bird, David M.
Vigil, Adam
Hung, Chris
Nakajima, Rie
Liang, Li
Trieu, Angela
Doolan, Denise L.
Thomas, Jennifer E.
Levy, Michael G.
Reichard, Mason V.
Felgner, Philip L.
Cohn, Leah A.
Birkenheuer, Adam J.
author_sort Schreeg, Megan E.
collection PubMed
description BACKGROUND: Cytauxzoonosis is a disease of felids in North America caused by the tick-transmitted apicomplexan parasite Cytauxzoon felis. Cytauxzoonosis is particularly virulent for domestic cats, but no vaccine currently exists. The parasite cannot be cultivated in vitro, presenting a significant limitation for vaccine development. METHODS: Recent sequencing of the C. felis genome has identified over 4300 putative protein-encoding genes. From this pool we constructed a protein microarray containing 673 putative C. felis proteins. This microarray was probed with sera from C. felis-infected and naïve cats to identify differentially reactive antigens which were incorporated into two expression library vaccines, one polyvalent and one monovalent. We assessed the efficacy of these vaccines to prevent of infection and/or disease in a tick-challenge model. RESULTS: Probing of the protein microarray resulted in identification of 30 differentially reactive C. felis antigens that were incorporated into the two expression library vaccines. However, expression library immunization failed to prevent infection or disease in cats challenged with C. felis. CONCLUSIONS: Protein microarray facilitated high-throughput identification of novel antigens, substantially increasing the pool of characterized C. felis antigens. These antigens should be considered for development of C. felis vaccines, diagnostics, and therapeutics. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12014-018-9218-9) contains supplementary material, which is available to authorized users.
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spelling pubmed-63109482019-01-07 Identification of Cytauxzoon felis antigens via protein microarray and assessment of expression library immunization against cytauxzoonosis Schreeg, Megan E. Marr, Henry S. Tarigo, Jaime L. Sherrill, Meredith K. Outi, Hilton K. Scholl, Elizabeth H. Bird, David M. Vigil, Adam Hung, Chris Nakajima, Rie Liang, Li Trieu, Angela Doolan, Denise L. Thomas, Jennifer E. Levy, Michael G. Reichard, Mason V. Felgner, Philip L. Cohn, Leah A. Birkenheuer, Adam J. Clin Proteomics Research BACKGROUND: Cytauxzoonosis is a disease of felids in North America caused by the tick-transmitted apicomplexan parasite Cytauxzoon felis. Cytauxzoonosis is particularly virulent for domestic cats, but no vaccine currently exists. The parasite cannot be cultivated in vitro, presenting a significant limitation for vaccine development. METHODS: Recent sequencing of the C. felis genome has identified over 4300 putative protein-encoding genes. From this pool we constructed a protein microarray containing 673 putative C. felis proteins. This microarray was probed with sera from C. felis-infected and naïve cats to identify differentially reactive antigens which were incorporated into two expression library vaccines, one polyvalent and one monovalent. We assessed the efficacy of these vaccines to prevent of infection and/or disease in a tick-challenge model. RESULTS: Probing of the protein microarray resulted in identification of 30 differentially reactive C. felis antigens that were incorporated into the two expression library vaccines. However, expression library immunization failed to prevent infection or disease in cats challenged with C. felis. CONCLUSIONS: Protein microarray facilitated high-throughput identification of novel antigens, substantially increasing the pool of characterized C. felis antigens. These antigens should be considered for development of C. felis vaccines, diagnostics, and therapeutics. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12014-018-9218-9) contains supplementary material, which is available to authorized users. BioMed Central 2018-12-29 /pmc/articles/PMC6310948/ /pubmed/30618510 http://dx.doi.org/10.1186/s12014-018-9218-9 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Schreeg, Megan E.
Marr, Henry S.
Tarigo, Jaime L.
Sherrill, Meredith K.
Outi, Hilton K.
Scholl, Elizabeth H.
Bird, David M.
Vigil, Adam
Hung, Chris
Nakajima, Rie
Liang, Li
Trieu, Angela
Doolan, Denise L.
Thomas, Jennifer E.
Levy, Michael G.
Reichard, Mason V.
Felgner, Philip L.
Cohn, Leah A.
Birkenheuer, Adam J.
Identification of Cytauxzoon felis antigens via protein microarray and assessment of expression library immunization against cytauxzoonosis
title Identification of Cytauxzoon felis antigens via protein microarray and assessment of expression library immunization against cytauxzoonosis
title_full Identification of Cytauxzoon felis antigens via protein microarray and assessment of expression library immunization against cytauxzoonosis
title_fullStr Identification of Cytauxzoon felis antigens via protein microarray and assessment of expression library immunization against cytauxzoonosis
title_full_unstemmed Identification of Cytauxzoon felis antigens via protein microarray and assessment of expression library immunization against cytauxzoonosis
title_short Identification of Cytauxzoon felis antigens via protein microarray and assessment of expression library immunization against cytauxzoonosis
title_sort identification of cytauxzoon felis antigens via protein microarray and assessment of expression library immunization against cytauxzoonosis
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6310948/
https://www.ncbi.nlm.nih.gov/pubmed/30618510
http://dx.doi.org/10.1186/s12014-018-9218-9
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