Homology-independent multiallelic disruption via CRISPR/Cas9-based knock-in yields distinct functional outcomes in human cells

BACKGROUND: Cultured human cells are pivotal models to study human gene functions, but introducing complete loss of function in diploid or aneuploid cells has been a challenge. The recently developed CRISPR/Cas9-mediated homology-independent knock-in approach permits targeted insertion of large DNA...

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Detalles Bibliográficos
Autores principales: Zhang, Chenzi, He, Xiangjun, Kwok, Yvonne K., Wang, Feng, Xue, Junyi, Zhao, Hui, Suen, Kin Wah, Wang, Chi Chiu, Ren, Jianwei, Chen, George G., Lai, Paul B. S., Li, Jiangchao, Xia, Yin, Chan, Andrew M., Chan, Wai-Yee, Feng, Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6310992/
https://www.ncbi.nlm.nih.gov/pubmed/30593266
http://dx.doi.org/10.1186/s12915-018-0616-2

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