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Alterations in the proteomic composition of Serratia marcescens in response to manganese (II)
BACKGROUND: Proteomics is an important tool for the investigation of dynamic physiological responses of microbes under heavy metal stress. To gain insight into how bacteria respond to manganese (II) and identify the proteins involved in Mn (II) oxidation, the shotgun proteomics approach was applied...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6311052/ https://www.ncbi.nlm.nih.gov/pubmed/30594179 http://dx.doi.org/10.1186/s12896-018-0493-3 |
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author | Queiroz, Pollyana Santos Ruas, France Anne Dias Barboza, Natália Rocha de Castro Borges, William Guerra-Sá, Renata |
author_facet | Queiroz, Pollyana Santos Ruas, France Anne Dias Barboza, Natália Rocha de Castro Borges, William Guerra-Sá, Renata |
author_sort | Queiroz, Pollyana Santos |
collection | PubMed |
description | BACKGROUND: Proteomics is an important tool for the investigation of dynamic physiological responses of microbes under heavy metal stress. To gain insight into how bacteria respond to manganese (II) and identify the proteins involved in Mn (II) oxidation, the shotgun proteomics approach was applied to a potential Mn (II)-oxidizing Serratia marcescens strain cultivated in the absence and presence of Mn (II). RESULTS: The LG1 strain, which grew equally well in the two conditions, was found to express a set of proteins related to cellular processes vital for survival, as well as proteins involved in adaptation and tolerance to Mn (II). The multicopper oxidase CueO was identified, indicating its probable participation in the Mn (II) bio-oxidation; however, its expression was not modulated by the presence of Mn (II). A set of proteins related to cell and metabolic processes vital to the cells were downregulated in the presence of Mn (II), while cell membrane-related proteins involved in the maintenance of cell integrity and survival under stress were upregulated under this condition. CONCLUSIONS: These findings indicate that the LG1 strain may be applied successfully in the bioremediation of Mn (II), and the shotgun approach provides an efficient means for obtaining the total proteome of this species. |
format | Online Article Text |
id | pubmed-6311052 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-63110522019-01-07 Alterations in the proteomic composition of Serratia marcescens in response to manganese (II) Queiroz, Pollyana Santos Ruas, France Anne Dias Barboza, Natália Rocha de Castro Borges, William Guerra-Sá, Renata BMC Biotechnol Research Article BACKGROUND: Proteomics is an important tool for the investigation of dynamic physiological responses of microbes under heavy metal stress. To gain insight into how bacteria respond to manganese (II) and identify the proteins involved in Mn (II) oxidation, the shotgun proteomics approach was applied to a potential Mn (II)-oxidizing Serratia marcescens strain cultivated in the absence and presence of Mn (II). RESULTS: The LG1 strain, which grew equally well in the two conditions, was found to express a set of proteins related to cellular processes vital for survival, as well as proteins involved in adaptation and tolerance to Mn (II). The multicopper oxidase CueO was identified, indicating its probable participation in the Mn (II) bio-oxidation; however, its expression was not modulated by the presence of Mn (II). A set of proteins related to cell and metabolic processes vital to the cells were downregulated in the presence of Mn (II), while cell membrane-related proteins involved in the maintenance of cell integrity and survival under stress were upregulated under this condition. CONCLUSIONS: These findings indicate that the LG1 strain may be applied successfully in the bioremediation of Mn (II), and the shotgun approach provides an efficient means for obtaining the total proteome of this species. BioMed Central 2018-12-29 /pmc/articles/PMC6311052/ /pubmed/30594179 http://dx.doi.org/10.1186/s12896-018-0493-3 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Queiroz, Pollyana Santos Ruas, France Anne Dias Barboza, Natália Rocha de Castro Borges, William Guerra-Sá, Renata Alterations in the proteomic composition of Serratia marcescens in response to manganese (II) |
title | Alterations in the proteomic composition of Serratia marcescens in response to manganese (II) |
title_full | Alterations in the proteomic composition of Serratia marcescens in response to manganese (II) |
title_fullStr | Alterations in the proteomic composition of Serratia marcescens in response to manganese (II) |
title_full_unstemmed | Alterations in the proteomic composition of Serratia marcescens in response to manganese (II) |
title_short | Alterations in the proteomic composition of Serratia marcescens in response to manganese (II) |
title_sort | alterations in the proteomic composition of serratia marcescens in response to manganese (ii) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6311052/ https://www.ncbi.nlm.nih.gov/pubmed/30594179 http://dx.doi.org/10.1186/s12896-018-0493-3 |
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