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Alterations in the proteomic composition of Serratia marcescens in response to manganese (II)

BACKGROUND: Proteomics is an important tool for the investigation of dynamic physiological responses of microbes under heavy metal stress. To gain insight into how bacteria respond to manganese (II) and identify the proteins involved in Mn (II) oxidation, the shotgun proteomics approach was applied...

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Autores principales: Queiroz, Pollyana Santos, Ruas, France Anne Dias, Barboza, Natália Rocha, de Castro Borges, William, Guerra-Sá, Renata
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6311052/
https://www.ncbi.nlm.nih.gov/pubmed/30594179
http://dx.doi.org/10.1186/s12896-018-0493-3
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author Queiroz, Pollyana Santos
Ruas, France Anne Dias
Barboza, Natália Rocha
de Castro Borges, William
Guerra-Sá, Renata
author_facet Queiroz, Pollyana Santos
Ruas, France Anne Dias
Barboza, Natália Rocha
de Castro Borges, William
Guerra-Sá, Renata
author_sort Queiroz, Pollyana Santos
collection PubMed
description BACKGROUND: Proteomics is an important tool for the investigation of dynamic physiological responses of microbes under heavy metal stress. To gain insight into how bacteria respond to manganese (II) and identify the proteins involved in Mn (II) oxidation, the shotgun proteomics approach was applied to a potential Mn (II)-oxidizing Serratia marcescens strain cultivated in the absence and presence of Mn (II). RESULTS: The LG1 strain, which grew equally well in the two conditions, was found to express a set of proteins related to cellular processes vital for survival, as well as proteins involved in adaptation and tolerance to Mn (II). The multicopper oxidase CueO was identified, indicating its probable participation in the Mn (II) bio-oxidation; however, its expression was not modulated by the presence of Mn (II). A set of proteins related to cell and metabolic processes vital to the cells were downregulated in the presence of Mn (II), while cell membrane-related proteins involved in the maintenance of cell integrity and survival under stress were upregulated under this condition. CONCLUSIONS: These findings indicate that the LG1 strain may be applied successfully in the bioremediation of Mn (II), and the shotgun approach provides an efficient means for obtaining the total proteome of this species.
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spelling pubmed-63110522019-01-07 Alterations in the proteomic composition of Serratia marcescens in response to manganese (II) Queiroz, Pollyana Santos Ruas, France Anne Dias Barboza, Natália Rocha de Castro Borges, William Guerra-Sá, Renata BMC Biotechnol Research Article BACKGROUND: Proteomics is an important tool for the investigation of dynamic physiological responses of microbes under heavy metal stress. To gain insight into how bacteria respond to manganese (II) and identify the proteins involved in Mn (II) oxidation, the shotgun proteomics approach was applied to a potential Mn (II)-oxidizing Serratia marcescens strain cultivated in the absence and presence of Mn (II). RESULTS: The LG1 strain, which grew equally well in the two conditions, was found to express a set of proteins related to cellular processes vital for survival, as well as proteins involved in adaptation and tolerance to Mn (II). The multicopper oxidase CueO was identified, indicating its probable participation in the Mn (II) bio-oxidation; however, its expression was not modulated by the presence of Mn (II). A set of proteins related to cell and metabolic processes vital to the cells were downregulated in the presence of Mn (II), while cell membrane-related proteins involved in the maintenance of cell integrity and survival under stress were upregulated under this condition. CONCLUSIONS: These findings indicate that the LG1 strain may be applied successfully in the bioremediation of Mn (II), and the shotgun approach provides an efficient means for obtaining the total proteome of this species. BioMed Central 2018-12-29 /pmc/articles/PMC6311052/ /pubmed/30594179 http://dx.doi.org/10.1186/s12896-018-0493-3 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Queiroz, Pollyana Santos
Ruas, France Anne Dias
Barboza, Natália Rocha
de Castro Borges, William
Guerra-Sá, Renata
Alterations in the proteomic composition of Serratia marcescens in response to manganese (II)
title Alterations in the proteomic composition of Serratia marcescens in response to manganese (II)
title_full Alterations in the proteomic composition of Serratia marcescens in response to manganese (II)
title_fullStr Alterations in the proteomic composition of Serratia marcescens in response to manganese (II)
title_full_unstemmed Alterations in the proteomic composition of Serratia marcescens in response to manganese (II)
title_short Alterations in the proteomic composition of Serratia marcescens in response to manganese (II)
title_sort alterations in the proteomic composition of serratia marcescens in response to manganese (ii)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6311052/
https://www.ncbi.nlm.nih.gov/pubmed/30594179
http://dx.doi.org/10.1186/s12896-018-0493-3
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