Cargando…
PLGA-based control release of Noggin blocks the premature fusion of cranial sutures caused by retinoic acid
Craniosynostosis (CS), the premature and pathological fusion of cranial sutures, is a relatively common developmental disorder. Elucidation of the pathways involved and thus therapeutically targeting it would be promising for the prevention of CS. In the present study, we examined the role of BMP pa...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6311184/ https://www.ncbi.nlm.nih.gov/pubmed/30392121 http://dx.doi.org/10.1007/s00253-018-9457-8 |
_version_ | 1783383563410866176 |
---|---|
author | Wang, Weicai Zhou, Chen Feng, Zhicai Li, Hongyu Zhang, Yadong Bao, Baicheng Cai, Bin Chen, Mu Huang, Hongzhang |
author_facet | Wang, Weicai Zhou, Chen Feng, Zhicai Li, Hongyu Zhang, Yadong Bao, Baicheng Cai, Bin Chen, Mu Huang, Hongzhang |
author_sort | Wang, Weicai |
collection | PubMed |
description | Craniosynostosis (CS), the premature and pathological fusion of cranial sutures, is a relatively common developmental disorder. Elucidation of the pathways involved and thus therapeutically targeting it would be promising for the prevention of CS. In the present study, we examined the role of BMP pathway in the all-trans retinoic acid (atRA)–induced CS model and tried to target the pathway in vivo via PLGA-based control release. As expected, the posterior frontal suture was found to fuse prematurely in the atRA subcutaneous injection mouse model. Further mechanism study revealed that atRA could repress the proliferation while promote the osteogenic differentiation of suture-derived mesenchymal cells (SMCs). Moreover, BMP signal pathway was found to be activated by atRA, as seen from increased expression of BMPR-2 and pSMAD1/5/9. Recombinant mouse Noggin blocked the atRA-induced enhancement of osteogenesis of SMCs in vitro. In vivo, PLGA microsphere encapsulated with Noggin significantly prevented the atRA-induced suture fusion. Collectively, these data support the hypothesis that BMP signaling is involved in retinoic acid–induced premature fusion of cranial sutures, while PLGA microsphere–based control release of Noggin emerges as a promising strategy for prevention of atRA-induced suture fusion. |
format | Online Article Text |
id | pubmed-6311184 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-63111842019-01-10 PLGA-based control release of Noggin blocks the premature fusion of cranial sutures caused by retinoic acid Wang, Weicai Zhou, Chen Feng, Zhicai Li, Hongyu Zhang, Yadong Bao, Baicheng Cai, Bin Chen, Mu Huang, Hongzhang Appl Microbiol Biotechnol Applied Genetics and Molecular Biotechnology Craniosynostosis (CS), the premature and pathological fusion of cranial sutures, is a relatively common developmental disorder. Elucidation of the pathways involved and thus therapeutically targeting it would be promising for the prevention of CS. In the present study, we examined the role of BMP pathway in the all-trans retinoic acid (atRA)–induced CS model and tried to target the pathway in vivo via PLGA-based control release. As expected, the posterior frontal suture was found to fuse prematurely in the atRA subcutaneous injection mouse model. Further mechanism study revealed that atRA could repress the proliferation while promote the osteogenic differentiation of suture-derived mesenchymal cells (SMCs). Moreover, BMP signal pathway was found to be activated by atRA, as seen from increased expression of BMPR-2 and pSMAD1/5/9. Recombinant mouse Noggin blocked the atRA-induced enhancement of osteogenesis of SMCs in vitro. In vivo, PLGA microsphere encapsulated with Noggin significantly prevented the atRA-induced suture fusion. Collectively, these data support the hypothesis that BMP signaling is involved in retinoic acid–induced premature fusion of cranial sutures, while PLGA microsphere–based control release of Noggin emerges as a promising strategy for prevention of atRA-induced suture fusion. Springer Berlin Heidelberg 2018-11-03 2019 /pmc/articles/PMC6311184/ /pubmed/30392121 http://dx.doi.org/10.1007/s00253-018-9457-8 Text en © The Author(s) 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Applied Genetics and Molecular Biotechnology Wang, Weicai Zhou, Chen Feng, Zhicai Li, Hongyu Zhang, Yadong Bao, Baicheng Cai, Bin Chen, Mu Huang, Hongzhang PLGA-based control release of Noggin blocks the premature fusion of cranial sutures caused by retinoic acid |
title | PLGA-based control release of Noggin blocks the premature fusion of cranial sutures caused by retinoic acid |
title_full | PLGA-based control release of Noggin blocks the premature fusion of cranial sutures caused by retinoic acid |
title_fullStr | PLGA-based control release of Noggin blocks the premature fusion of cranial sutures caused by retinoic acid |
title_full_unstemmed | PLGA-based control release of Noggin blocks the premature fusion of cranial sutures caused by retinoic acid |
title_short | PLGA-based control release of Noggin blocks the premature fusion of cranial sutures caused by retinoic acid |
title_sort | plga-based control release of noggin blocks the premature fusion of cranial sutures caused by retinoic acid |
topic | Applied Genetics and Molecular Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6311184/ https://www.ncbi.nlm.nih.gov/pubmed/30392121 http://dx.doi.org/10.1007/s00253-018-9457-8 |
work_keys_str_mv | AT wangweicai plgabasedcontrolreleaseofnogginblockstheprematurefusionofcranialsuturescausedbyretinoicacid AT zhouchen plgabasedcontrolreleaseofnogginblockstheprematurefusionofcranialsuturescausedbyretinoicacid AT fengzhicai plgabasedcontrolreleaseofnogginblockstheprematurefusionofcranialsuturescausedbyretinoicacid AT lihongyu plgabasedcontrolreleaseofnogginblockstheprematurefusionofcranialsuturescausedbyretinoicacid AT zhangyadong plgabasedcontrolreleaseofnogginblockstheprematurefusionofcranialsuturescausedbyretinoicacid AT baobaicheng plgabasedcontrolreleaseofnogginblockstheprematurefusionofcranialsuturescausedbyretinoicacid AT caibin plgabasedcontrolreleaseofnogginblockstheprematurefusionofcranialsuturescausedbyretinoicacid AT chenmu plgabasedcontrolreleaseofnogginblockstheprematurefusionofcranialsuturescausedbyretinoicacid AT huanghongzhang plgabasedcontrolreleaseofnogginblockstheprematurefusionofcranialsuturescausedbyretinoicacid |