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Tricholoma matsutake may take more nitrogen in the organic form than other ectomycorrhizal fungi for its sporocarp development: the isotopic evidence

Tricholoma matsutake is an ectomycorrhizal (ECM) fungus capable of in vitro saprotrophic growth, but the sources of C and N used to generate sporocarps in vivo are not well understood. We examined natural abundance isotope data to investigate this phenomenon. For this purpose, C, N and their stable...

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Autores principales: Vaario, Lu-Min, Sah, Shambhu Prasad, Norisada, Mariko, Narimatsu, Maki, Matsushita, Norihisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6311186/
https://www.ncbi.nlm.nih.gov/pubmed/30406843
http://dx.doi.org/10.1007/s00572-018-0870-8
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author Vaario, Lu-Min
Sah, Shambhu Prasad
Norisada, Mariko
Narimatsu, Maki
Matsushita, Norihisa
author_facet Vaario, Lu-Min
Sah, Shambhu Prasad
Norisada, Mariko
Narimatsu, Maki
Matsushita, Norihisa
author_sort Vaario, Lu-Min
collection PubMed
description Tricholoma matsutake is an ectomycorrhizal (ECM) fungus capable of in vitro saprotrophic growth, but the sources of C and N used to generate sporocarps in vivo are not well understood. We examined natural abundance isotope data to investigate this phenomenon. For this purpose, C, N and their stable isotopes ((13)C, (15)N) content of fungal sporocarps and their potential nutrient sources (i.e., foliage, litter, fine roots, wood, and soil) were investigated from two well-studied sites in Finland and Japan. Our results show that δ(13)C values of T. matsutake and other fungal groups are consistent with those of most studies, but a very high δ(15)N value (16.8‰ ± 2.3) is observed in T. matsutake. Such isotopic pattern of fungal δ(15)N suggests that matsutake has a greater proteolytic potential to digest chemically complex (15)N-enriched organic matter and hydrophobic hyphae. This assumption is further supported by a significant and positive correlation between δ(13)C(cap–stipe) and δ(15)N(cap–stipe) exclusively in T. matsutake, which suggests common C and N sources (protein) possible for isotopically enriched cap. The (13)C increase of caps relative to stipe presumably reflects greater contents of (13)C-enriched protein than (13)C-depleted chitin. We conclude that T. matsutake is a typical ECM fungus which obtains for its sporocarp development for both C and N from a common protein source (vs. photosynthetic carbon) present in soil organic matter. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00572-018-0870-8) contains supplementary material, which is available to authorized users.
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spelling pubmed-63111862019-01-10 Tricholoma matsutake may take more nitrogen in the organic form than other ectomycorrhizal fungi for its sporocarp development: the isotopic evidence Vaario, Lu-Min Sah, Shambhu Prasad Norisada, Mariko Narimatsu, Maki Matsushita, Norihisa Mycorrhiza Original Article Tricholoma matsutake is an ectomycorrhizal (ECM) fungus capable of in vitro saprotrophic growth, but the sources of C and N used to generate sporocarps in vivo are not well understood. We examined natural abundance isotope data to investigate this phenomenon. For this purpose, C, N and their stable isotopes ((13)C, (15)N) content of fungal sporocarps and their potential nutrient sources (i.e., foliage, litter, fine roots, wood, and soil) were investigated from two well-studied sites in Finland and Japan. Our results show that δ(13)C values of T. matsutake and other fungal groups are consistent with those of most studies, but a very high δ(15)N value (16.8‰ ± 2.3) is observed in T. matsutake. Such isotopic pattern of fungal δ(15)N suggests that matsutake has a greater proteolytic potential to digest chemically complex (15)N-enriched organic matter and hydrophobic hyphae. This assumption is further supported by a significant and positive correlation between δ(13)C(cap–stipe) and δ(15)N(cap–stipe) exclusively in T. matsutake, which suggests common C and N sources (protein) possible for isotopically enriched cap. The (13)C increase of caps relative to stipe presumably reflects greater contents of (13)C-enriched protein than (13)C-depleted chitin. We conclude that T. matsutake is a typical ECM fungus which obtains for its sporocarp development for both C and N from a common protein source (vs. photosynthetic carbon) present in soil organic matter. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00572-018-0870-8) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2018-11-08 2019 /pmc/articles/PMC6311186/ /pubmed/30406843 http://dx.doi.org/10.1007/s00572-018-0870-8 Text en © The Author(s) 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Vaario, Lu-Min
Sah, Shambhu Prasad
Norisada, Mariko
Narimatsu, Maki
Matsushita, Norihisa
Tricholoma matsutake may take more nitrogen in the organic form than other ectomycorrhizal fungi for its sporocarp development: the isotopic evidence
title Tricholoma matsutake may take more nitrogen in the organic form than other ectomycorrhizal fungi for its sporocarp development: the isotopic evidence
title_full Tricholoma matsutake may take more nitrogen in the organic form than other ectomycorrhizal fungi for its sporocarp development: the isotopic evidence
title_fullStr Tricholoma matsutake may take more nitrogen in the organic form than other ectomycorrhizal fungi for its sporocarp development: the isotopic evidence
title_full_unstemmed Tricholoma matsutake may take more nitrogen in the organic form than other ectomycorrhizal fungi for its sporocarp development: the isotopic evidence
title_short Tricholoma matsutake may take more nitrogen in the organic form than other ectomycorrhizal fungi for its sporocarp development: the isotopic evidence
title_sort tricholoma matsutake may take more nitrogen in the organic form than other ectomycorrhizal fungi for its sporocarp development: the isotopic evidence
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6311186/
https://www.ncbi.nlm.nih.gov/pubmed/30406843
http://dx.doi.org/10.1007/s00572-018-0870-8
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