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Gossypol Promotes Bone Formation in Ovariectomy-Induced Osteoporosis through Regulating Cell Apoptosis

Osteoporosis is among the most common forms of age-related diseases, especially for females, which has been a grave public health problem. Drug therapies have shown promising outcomes to promote bone formation and bone density. This study identified a novel potential drug, gossypol, for the treatmen...

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Autores principales: Liang, Jinqian, Chen, Chong, Liu, Hongzhe, Liu, Xiangyang, Li, Zheng, Hu, Jianhua, Zhao, Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6311247/
https://www.ncbi.nlm.nih.gov/pubmed/30643801
http://dx.doi.org/10.1155/2018/3635485
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author Liang, Jinqian
Chen, Chong
Liu, Hongzhe
Liu, Xiangyang
Li, Zheng
Hu, Jianhua
Zhao, Hong
author_facet Liang, Jinqian
Chen, Chong
Liu, Hongzhe
Liu, Xiangyang
Li, Zheng
Hu, Jianhua
Zhao, Hong
author_sort Liang, Jinqian
collection PubMed
description Osteoporosis is among the most common forms of age-related diseases, especially for females, which has been a grave public health problem. Drug therapies have shown promising outcomes to promote bone formation and bone density. This study identified a novel potential drug, gossypol, for the treatment of osteoporosis. Treatments of ovariectomy-induced osteoporosis mice with gossypol significantly increased serum osteocalcin and osteoprotegerin (OPG) levels; meanwhile they decreased serum RANKL levels. Microcomputed tomography (microCT) analysis showed that treatment of gossypol improved bone density and strength and decreased bone postyield displacement for both medullar and cortical bones. In vitro experiments also showed that gossypol increased cell viability in a time- and dose-dependent manner. Furthermore, incubation of the osteoblast MC3T3-E1 cells with gossypol inhibited cell apoptosis through intrinsic apoptotic pathway as evidenced by the Annexin V/PI assay, TUNEL assay, biochemical analysis, and western blot assays. Moreover, the classical Wnt/β-catenin signaling pathway was found to be regulated by gossypol treatments. Inhibition of Wnt/β-catenin signaling reversed the prevention effects of gossypol in osteoporosis. Our findings provided novel clues for the treatment of osteoporosis in clinic.
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spelling pubmed-63112472019-01-14 Gossypol Promotes Bone Formation in Ovariectomy-Induced Osteoporosis through Regulating Cell Apoptosis Liang, Jinqian Chen, Chong Liu, Hongzhe Liu, Xiangyang Li, Zheng Hu, Jianhua Zhao, Hong Biomed Res Int Research Article Osteoporosis is among the most common forms of age-related diseases, especially for females, which has been a grave public health problem. Drug therapies have shown promising outcomes to promote bone formation and bone density. This study identified a novel potential drug, gossypol, for the treatment of osteoporosis. Treatments of ovariectomy-induced osteoporosis mice with gossypol significantly increased serum osteocalcin and osteoprotegerin (OPG) levels; meanwhile they decreased serum RANKL levels. Microcomputed tomography (microCT) analysis showed that treatment of gossypol improved bone density and strength and decreased bone postyield displacement for both medullar and cortical bones. In vitro experiments also showed that gossypol increased cell viability in a time- and dose-dependent manner. Furthermore, incubation of the osteoblast MC3T3-E1 cells with gossypol inhibited cell apoptosis through intrinsic apoptotic pathway as evidenced by the Annexin V/PI assay, TUNEL assay, biochemical analysis, and western blot assays. Moreover, the classical Wnt/β-catenin signaling pathway was found to be regulated by gossypol treatments. Inhibition of Wnt/β-catenin signaling reversed the prevention effects of gossypol in osteoporosis. Our findings provided novel clues for the treatment of osteoporosis in clinic. Hindawi 2018-12-13 /pmc/articles/PMC6311247/ /pubmed/30643801 http://dx.doi.org/10.1155/2018/3635485 Text en Copyright © 2018 Jinqian Liang et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Liang, Jinqian
Chen, Chong
Liu, Hongzhe
Liu, Xiangyang
Li, Zheng
Hu, Jianhua
Zhao, Hong
Gossypol Promotes Bone Formation in Ovariectomy-Induced Osteoporosis through Regulating Cell Apoptosis
title Gossypol Promotes Bone Formation in Ovariectomy-Induced Osteoporosis through Regulating Cell Apoptosis
title_full Gossypol Promotes Bone Formation in Ovariectomy-Induced Osteoporosis through Regulating Cell Apoptosis
title_fullStr Gossypol Promotes Bone Formation in Ovariectomy-Induced Osteoporosis through Regulating Cell Apoptosis
title_full_unstemmed Gossypol Promotes Bone Formation in Ovariectomy-Induced Osteoporosis through Regulating Cell Apoptosis
title_short Gossypol Promotes Bone Formation in Ovariectomy-Induced Osteoporosis through Regulating Cell Apoptosis
title_sort gossypol promotes bone formation in ovariectomy-induced osteoporosis through regulating cell apoptosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6311247/
https://www.ncbi.nlm.nih.gov/pubmed/30643801
http://dx.doi.org/10.1155/2018/3635485
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