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Pseudomonas aeruginosa - Modified Hodge Test (PAE-MHT) and ChromID Carba Agar for Detection of Carbapenemase Producing Pseudomonas Aeruginosa Recovered from Clinical Specimens
AIMS: This study aims to evaluate the ability of ChromID Carba agar, and Pseudomonas aeruginosa modified Hodge test (PAE-MHT) for detection of carbapenemase-producing P. aeruginosa and to determine the associated carbapenemase gene classes by PCR. METHODS: One hundred Carbapenem-resistant P. aerugin...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Republic of Macedonia
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6311476/ https://www.ncbi.nlm.nih.gov/pubmed/30607177 http://dx.doi.org/10.3889/oamjms.2018.414 |
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author | Othman, Hala B. Halim, Rania Mohamed Abdel Abdul-Wahab, Hoda Ezz El-arab Atta, Hossam Abol Shaaban, Omyma |
author_facet | Othman, Hala B. Halim, Rania Mohamed Abdel Abdul-Wahab, Hoda Ezz El-arab Atta, Hossam Abol Shaaban, Omyma |
author_sort | Othman, Hala B. |
collection | PubMed |
description | AIMS: This study aims to evaluate the ability of ChromID Carba agar, and Pseudomonas aeruginosa modified Hodge test (PAE-MHT) for detection of carbapenemase-producing P. aeruginosa and to determine the associated carbapenemase gene classes by PCR. METHODS: One hundred Carbapenem-resistant P. aeruginosa (CRPA) isolates were tested for: i) carbapenemases production by ChromID carba agar, Modified Hodge test (MHT) and (PAE-MHT) and ii) detection of some carbapenemase genes by PCR. RESULTS: All (100%) of the isolates showed growth on ChromID Carba agar with 100% sensitivity. Using MHT, 54% of isolates were positive, 3% were indeterminate, and 43% were negative, demonstrating 58.9% sensitivity and 80% specificity. On performing PAE-MHT, 91% of the strains were positive, 3% were intermediate, and 6% were negative, demonstrating 97.9% sensitivity and 80% specificity. The most prevalent gene was blaKPC (81%), followed by blaVIM (74%); blaIMP was detected in only one isolate, and blaOXA-48 in 34% of the isolates. CONCLUSIONS: We conclude that PAE-MHT and ChromID Carba are sensitive, specific, simple and cost-effective screening tests for detection of CRPA isolates compared to the traditional MHT. |
format | Online Article Text |
id | pubmed-6311476 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Republic of Macedonia |
record_format | MEDLINE/PubMed |
spelling | pubmed-63114762019-01-03 Pseudomonas aeruginosa - Modified Hodge Test (PAE-MHT) and ChromID Carba Agar for Detection of Carbapenemase Producing Pseudomonas Aeruginosa Recovered from Clinical Specimens Othman, Hala B. Halim, Rania Mohamed Abdel Abdul-Wahab, Hoda Ezz El-arab Atta, Hossam Abol Shaaban, Omyma Open Access Maced J Med Sci Basic Science AIMS: This study aims to evaluate the ability of ChromID Carba agar, and Pseudomonas aeruginosa modified Hodge test (PAE-MHT) for detection of carbapenemase-producing P. aeruginosa and to determine the associated carbapenemase gene classes by PCR. METHODS: One hundred Carbapenem-resistant P. aeruginosa (CRPA) isolates were tested for: i) carbapenemases production by ChromID carba agar, Modified Hodge test (MHT) and (PAE-MHT) and ii) detection of some carbapenemase genes by PCR. RESULTS: All (100%) of the isolates showed growth on ChromID Carba agar with 100% sensitivity. Using MHT, 54% of isolates were positive, 3% were indeterminate, and 43% were negative, demonstrating 58.9% sensitivity and 80% specificity. On performing PAE-MHT, 91% of the strains were positive, 3% were intermediate, and 6% were negative, demonstrating 97.9% sensitivity and 80% specificity. The most prevalent gene was blaKPC (81%), followed by blaVIM (74%); blaIMP was detected in only one isolate, and blaOXA-48 in 34% of the isolates. CONCLUSIONS: We conclude that PAE-MHT and ChromID Carba are sensitive, specific, simple and cost-effective screening tests for detection of CRPA isolates compared to the traditional MHT. Republic of Macedonia 2018-12-15 /pmc/articles/PMC6311476/ /pubmed/30607177 http://dx.doi.org/10.3889/oamjms.2018.414 Text en Copyright: © 2018 Hala B. Othman, Rania Mohamed Abdel Halim, Hoda Ezz El-arab Abdul-wahab, Hossam Abol Atta, Omyma Shaaban. http://creativecommons.org/licenses/CC BY-NC/4.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0). |
spellingShingle | Basic Science Othman, Hala B. Halim, Rania Mohamed Abdel Abdul-Wahab, Hoda Ezz El-arab Atta, Hossam Abol Shaaban, Omyma Pseudomonas aeruginosa - Modified Hodge Test (PAE-MHT) and ChromID Carba Agar for Detection of Carbapenemase Producing Pseudomonas Aeruginosa Recovered from Clinical Specimens |
title | Pseudomonas aeruginosa - Modified Hodge Test (PAE-MHT) and ChromID Carba Agar for Detection of Carbapenemase Producing Pseudomonas Aeruginosa Recovered from Clinical Specimens |
title_full | Pseudomonas aeruginosa - Modified Hodge Test (PAE-MHT) and ChromID Carba Agar for Detection of Carbapenemase Producing Pseudomonas Aeruginosa Recovered from Clinical Specimens |
title_fullStr | Pseudomonas aeruginosa - Modified Hodge Test (PAE-MHT) and ChromID Carba Agar for Detection of Carbapenemase Producing Pseudomonas Aeruginosa Recovered from Clinical Specimens |
title_full_unstemmed | Pseudomonas aeruginosa - Modified Hodge Test (PAE-MHT) and ChromID Carba Agar for Detection of Carbapenemase Producing Pseudomonas Aeruginosa Recovered from Clinical Specimens |
title_short | Pseudomonas aeruginosa - Modified Hodge Test (PAE-MHT) and ChromID Carba Agar for Detection of Carbapenemase Producing Pseudomonas Aeruginosa Recovered from Clinical Specimens |
title_sort | pseudomonas aeruginosa - modified hodge test (pae-mht) and chromid carba agar for detection of carbapenemase producing pseudomonas aeruginosa recovered from clinical specimens |
topic | Basic Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6311476/ https://www.ncbi.nlm.nih.gov/pubmed/30607177 http://dx.doi.org/10.3889/oamjms.2018.414 |
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