Cargando…
Streptomyces Levis ABRIINW111 Inhibits SW480 Cells Growth by Apoptosis Induction
Purpose: Streptomyces sp., a dominant genus in Actinomycetes, is the source of a wide variety of secondary metabolites. Microbial metabolites can be utilized as novel anticancer agents; with fewer side effects. The present article illustrated the anti-carcinogenic effect of the ether extracted organ...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Tabriz University of Medical Sciences
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6311630/ https://www.ncbi.nlm.nih.gov/pubmed/30607340 http://dx.doi.org/10.15171/apb.2018.076 |
_version_ | 1783383639869882368 |
---|---|
author | Faramarzian Azimi Maragheh, Behnaz Fatourachi, Parisa Mohammadi, Seyede Momeneh Valipour, Behnaz Behtari, Meysam Dehnad, Alireza Nozad Charoudeh, Hojjatollah |
author_facet | Faramarzian Azimi Maragheh, Behnaz Fatourachi, Parisa Mohammadi, Seyede Momeneh Valipour, Behnaz Behtari, Meysam Dehnad, Alireza Nozad Charoudeh, Hojjatollah |
author_sort | Faramarzian Azimi Maragheh, Behnaz |
collection | PubMed |
description | Purpose: Streptomyces sp., a dominant genus in Actinomycetes, is the source of a wide variety of secondary metabolites. Microbial metabolites can be utilized as novel anticancer agents; with fewer side effects. The present article illustrated the anti-carcinogenic effect of the ether extracted organic metabolites derived from Streptomyces bacteria on SW480 colon cancer cell line. Methods: MTT assay was performed in order to investigate the cytotoxicity effect of metabolites on SW480 cells. Apoptosis and cell cycle arrests were measured by flowcytometry. Morphological changes were indicated by Propidium iodide staining andP53 gene expression was evaluated by real-time PCR. Results: Streptomyces Levis ABRIINW111 inhibited cell growth, increased Caspases 3 and reduced Ki67 expression in a concentration/time-dependent manner in SW40 cells. Metabolites increased subG1 phase (apoptosis) and also cell cycle arrest in G1, G2/M and S phase. P53 gene expression followed Sw480 cells treatment significantly. Conclusion: Streptomyces sp. metabolites have anti-carcinogenic effect on colon cancer cells. Streptomyces Levis ABRIINW111 metabolites are a candidate for Colon cancer treatment. |
format | Online Article Text |
id | pubmed-6311630 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Tabriz University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-63116302019-01-03 Streptomyces Levis ABRIINW111 Inhibits SW480 Cells Growth by Apoptosis Induction Faramarzian Azimi Maragheh, Behnaz Fatourachi, Parisa Mohammadi, Seyede Momeneh Valipour, Behnaz Behtari, Meysam Dehnad, Alireza Nozad Charoudeh, Hojjatollah Adv Pharm Bull Research Article Purpose: Streptomyces sp., a dominant genus in Actinomycetes, is the source of a wide variety of secondary metabolites. Microbial metabolites can be utilized as novel anticancer agents; with fewer side effects. The present article illustrated the anti-carcinogenic effect of the ether extracted organic metabolites derived from Streptomyces bacteria on SW480 colon cancer cell line. Methods: MTT assay was performed in order to investigate the cytotoxicity effect of metabolites on SW480 cells. Apoptosis and cell cycle arrests were measured by flowcytometry. Morphological changes were indicated by Propidium iodide staining andP53 gene expression was evaluated by real-time PCR. Results: Streptomyces Levis ABRIINW111 inhibited cell growth, increased Caspases 3 and reduced Ki67 expression in a concentration/time-dependent manner in SW40 cells. Metabolites increased subG1 phase (apoptosis) and also cell cycle arrest in G1, G2/M and S phase. P53 gene expression followed Sw480 cells treatment significantly. Conclusion: Streptomyces sp. metabolites have anti-carcinogenic effect on colon cancer cells. Streptomyces Levis ABRIINW111 metabolites are a candidate for Colon cancer treatment. Tabriz University of Medical Sciences 2018-11 2018-11-29 /pmc/articles/PMC6311630/ /pubmed/30607340 http://dx.doi.org/10.15171/apb.2018.076 Text en ©2018 The Authors. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution (CC BY), which permits unrestricted use, distribution, and reproduction in any medium, as long as the original authors and source are cited. No permission is required from the authors or the publishers. |
spellingShingle | Research Article Faramarzian Azimi Maragheh, Behnaz Fatourachi, Parisa Mohammadi, Seyede Momeneh Valipour, Behnaz Behtari, Meysam Dehnad, Alireza Nozad Charoudeh, Hojjatollah Streptomyces Levis ABRIINW111 Inhibits SW480 Cells Growth by Apoptosis Induction |
title | Streptomyces Levis ABRIINW111 Inhibits SW480 Cells Growth by Apoptosis Induction |
title_full | Streptomyces Levis ABRIINW111 Inhibits SW480 Cells Growth by Apoptosis Induction |
title_fullStr | Streptomyces Levis ABRIINW111 Inhibits SW480 Cells Growth by Apoptosis Induction |
title_full_unstemmed | Streptomyces Levis ABRIINW111 Inhibits SW480 Cells Growth by Apoptosis Induction |
title_short | Streptomyces Levis ABRIINW111 Inhibits SW480 Cells Growth by Apoptosis Induction |
title_sort | streptomyces levis abriinw111 inhibits sw480 cells growth by apoptosis induction |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6311630/ https://www.ncbi.nlm.nih.gov/pubmed/30607340 http://dx.doi.org/10.15171/apb.2018.076 |
work_keys_str_mv | AT faramarzianazimimaraghehbehnaz streptomyceslevisabriinw111inhibitssw480cellsgrowthbyapoptosisinduction AT fatourachiparisa streptomyceslevisabriinw111inhibitssw480cellsgrowthbyapoptosisinduction AT mohammadiseyedemomeneh streptomyceslevisabriinw111inhibitssw480cellsgrowthbyapoptosisinduction AT valipourbehnaz streptomyceslevisabriinw111inhibitssw480cellsgrowthbyapoptosisinduction AT behtarimeysam streptomyceslevisabriinw111inhibitssw480cellsgrowthbyapoptosisinduction AT dehnadalireza streptomyceslevisabriinw111inhibitssw480cellsgrowthbyapoptosisinduction AT nozadcharoudehhojjatollah streptomyceslevisabriinw111inhibitssw480cellsgrowthbyapoptosisinduction |