A Turn-On Fluorescent Probe for Sensitive Detection of Cysteine in a Fully Aqueous Environment and in Living Cells

We reported here a turn-on fluorescent probe (1) for the detection of cysteine (Cys) by incorporating the recognition unit of 2,4-dinitrobenzenesulfonyl ester (DNBS) to a coumarin derivative. The structure of the obtained probe was confirmed by NMR and HRMS techniques. The probe shows a remarkable f...

Descripción completa

Detalles Bibliográficos
Autores principales: Ma, Xiaohua, Wu, Guoguang, Zhao, Yuehua, Yuan, Zibo, Zhang, Yu, Xia, Ning, Yang, Mengnan, Liu, Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6311829/
https://www.ncbi.nlm.nih.gov/pubmed/30647984
http://dx.doi.org/10.1155/2018/1986468
_version_ 1783383679093964800
author Ma, Xiaohua
Wu, Guoguang
Zhao, Yuehua
Yuan, Zibo
Zhang, Yu
Xia, Ning
Yang, Mengnan
Liu, Lin
author_facet Ma, Xiaohua
Wu, Guoguang
Zhao, Yuehua
Yuan, Zibo
Zhang, Yu
Xia, Ning
Yang, Mengnan
Liu, Lin
author_sort Ma, Xiaohua
collection PubMed
description We reported here a turn-on fluorescent probe (1) for the detection of cysteine (Cys) by incorporating the recognition unit of 2,4-dinitrobenzenesulfonyl ester (DNBS) to a coumarin derivative. The structure of the obtained probe was confirmed by NMR and HRMS techniques. The probe shows a remarkable fluorescence off-on response (∼52-fold) by the reaction with Cys in 100% aqueous buffer. The sensing mechanism was verified by the HPLC test. Probe 1 also displays high selectivity towards Cys. The detection limit was calculated to be 23 nM. Moreover, cellular experiments demonstrated that the probe is highly biocompatible and can be used for monitoring intracellular Cys.
format Online
Article
Text
id pubmed-6311829
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-63118292019-01-15 A Turn-On Fluorescent Probe for Sensitive Detection of Cysteine in a Fully Aqueous Environment and in Living Cells Ma, Xiaohua Wu, Guoguang Zhao, Yuehua Yuan, Zibo Zhang, Yu Xia, Ning Yang, Mengnan Liu, Lin J Anal Methods Chem Research Article We reported here a turn-on fluorescent probe (1) for the detection of cysteine (Cys) by incorporating the recognition unit of 2,4-dinitrobenzenesulfonyl ester (DNBS) to a coumarin derivative. The structure of the obtained probe was confirmed by NMR and HRMS techniques. The probe shows a remarkable fluorescence off-on response (∼52-fold) by the reaction with Cys in 100% aqueous buffer. The sensing mechanism was verified by the HPLC test. Probe 1 also displays high selectivity towards Cys. The detection limit was calculated to be 23 nM. Moreover, cellular experiments demonstrated that the probe is highly biocompatible and can be used for monitoring intracellular Cys. Hindawi 2018-12-13 /pmc/articles/PMC6311829/ /pubmed/30647984 http://dx.doi.org/10.1155/2018/1986468 Text en Copyright © 2018 Xiaohua Ma et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ma, Xiaohua
Wu, Guoguang
Zhao, Yuehua
Yuan, Zibo
Zhang, Yu
Xia, Ning
Yang, Mengnan
Liu, Lin
A Turn-On Fluorescent Probe for Sensitive Detection of Cysteine in a Fully Aqueous Environment and in Living Cells
title A Turn-On Fluorescent Probe for Sensitive Detection of Cysteine in a Fully Aqueous Environment and in Living Cells
title_full A Turn-On Fluorescent Probe for Sensitive Detection of Cysteine in a Fully Aqueous Environment and in Living Cells
title_fullStr A Turn-On Fluorescent Probe for Sensitive Detection of Cysteine in a Fully Aqueous Environment and in Living Cells
title_full_unstemmed A Turn-On Fluorescent Probe for Sensitive Detection of Cysteine in a Fully Aqueous Environment and in Living Cells
title_short A Turn-On Fluorescent Probe for Sensitive Detection of Cysteine in a Fully Aqueous Environment and in Living Cells
title_sort turn-on fluorescent probe for sensitive detection of cysteine in a fully aqueous environment and in living cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6311829/
https://www.ncbi.nlm.nih.gov/pubmed/30647984
http://dx.doi.org/10.1155/2018/1986468
work_keys_str_mv AT maxiaohua aturnonfluorescentprobeforsensitivedetectionofcysteineinafullyaqueousenvironmentandinlivingcells
AT wuguoguang aturnonfluorescentprobeforsensitivedetectionofcysteineinafullyaqueousenvironmentandinlivingcells
AT zhaoyuehua aturnonfluorescentprobeforsensitivedetectionofcysteineinafullyaqueousenvironmentandinlivingcells
AT yuanzibo aturnonfluorescentprobeforsensitivedetectionofcysteineinafullyaqueousenvironmentandinlivingcells
AT zhangyu aturnonfluorescentprobeforsensitivedetectionofcysteineinafullyaqueousenvironmentandinlivingcells
AT xianing aturnonfluorescentprobeforsensitivedetectionofcysteineinafullyaqueousenvironmentandinlivingcells
AT yangmengnan aturnonfluorescentprobeforsensitivedetectionofcysteineinafullyaqueousenvironmentandinlivingcells
AT liulin aturnonfluorescentprobeforsensitivedetectionofcysteineinafullyaqueousenvironmentandinlivingcells
AT maxiaohua turnonfluorescentprobeforsensitivedetectionofcysteineinafullyaqueousenvironmentandinlivingcells
AT wuguoguang turnonfluorescentprobeforsensitivedetectionofcysteineinafullyaqueousenvironmentandinlivingcells
AT zhaoyuehua turnonfluorescentprobeforsensitivedetectionofcysteineinafullyaqueousenvironmentandinlivingcells
AT yuanzibo turnonfluorescentprobeforsensitivedetectionofcysteineinafullyaqueousenvironmentandinlivingcells
AT zhangyu turnonfluorescentprobeforsensitivedetectionofcysteineinafullyaqueousenvironmentandinlivingcells
AT xianing turnonfluorescentprobeforsensitivedetectionofcysteineinafullyaqueousenvironmentandinlivingcells
AT yangmengnan turnonfluorescentprobeforsensitivedetectionofcysteineinafullyaqueousenvironmentandinlivingcells
AT liulin turnonfluorescentprobeforsensitivedetectionofcysteineinafullyaqueousenvironmentandinlivingcells

Ejemplares similares