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Total HLA Class I Antigen Loss with the Downregulation of Antigen-Processing Machinery Components in Two Newly Established Sarcomatoid Hepatocellular Carcinoma Cell Lines
Limited information is currently available concerning HLA class I antigen abnormalities in sarcomatoid hepatocellular carcinoma (sHCC). Here, we have analyzed the growth characteristics and HLA class I antigen status of four sHCC cell lines (sHCC29, sHCC63, sHCC74, and SAR-HCV); the first three were...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Hindawi
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6311956/ https://www.ncbi.nlm.nih.gov/pubmed/30648121 http://dx.doi.org/10.1155/2018/8363265 |
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author | Lei, Wei-Yi Hsiung, Shih-Chieh Wen, Shao-Hsuan Hsieh, Chin-Hsuan Chen, Chien-Lin Wallace, Christopher Glenn Chang, Chien-Chung Liao, Shuen-Kuei |
author_facet | Lei, Wei-Yi Hsiung, Shih-Chieh Wen, Shao-Hsuan Hsieh, Chin-Hsuan Chen, Chien-Lin Wallace, Christopher Glenn Chang, Chien-Chung Liao, Shuen-Kuei |
author_sort | Lei, Wei-Yi |
collection | PubMed |
description | Limited information is currently available concerning HLA class I antigen abnormalities in sarcomatoid hepatocellular carcinoma (sHCC). Here, we have analyzed the growth characteristics and HLA class I antigen status of four sHCC cell lines (sHCC29, sHCC63, sHCC74, and SAR-HCV); the first three were newly established in this study. Among the four, sHCC29 showed the highest growth rate in vitro and tumorigenicity in NOD-SCID mice. Unlike sHCC74 and SAR-HCV, both sHCC29 and sHCC63 had no detectable surface HLA class I antigen expression, alongside undetected intracellular β (2)-microglobulin (β (2)m) and marked HLA class I heavy chain and selective antigen-processing machinery (APM) component downregulation. The loss of β (2)m in sHCC29 and sHCC63 was caused by a >49 kb deletion across the B2M locus, while their downregulation of APM components was transcriptional, reversible by IFN-γ only in several components. β (2)m was also undetected in the primary HCC lesions of the patients involved, indicating its in vivo relevance. We report for the first time HLA class I antigen loss with underlying B2M gene deficiency and APM defects in 50% (2 of 4) of the sHCC cell lines tested. These findings may have implications for a proper design of T cell immunotherapy for the treatment of sHCC patients. |
format | Online Article Text |
id | pubmed-6311956 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-63119562019-01-15 Total HLA Class I Antigen Loss with the Downregulation of Antigen-Processing Machinery Components in Two Newly Established Sarcomatoid Hepatocellular Carcinoma Cell Lines Lei, Wei-Yi Hsiung, Shih-Chieh Wen, Shao-Hsuan Hsieh, Chin-Hsuan Chen, Chien-Lin Wallace, Christopher Glenn Chang, Chien-Chung Liao, Shuen-Kuei J Immunol Res Research Article Limited information is currently available concerning HLA class I antigen abnormalities in sarcomatoid hepatocellular carcinoma (sHCC). Here, we have analyzed the growth characteristics and HLA class I antigen status of four sHCC cell lines (sHCC29, sHCC63, sHCC74, and SAR-HCV); the first three were newly established in this study. Among the four, sHCC29 showed the highest growth rate in vitro and tumorigenicity in NOD-SCID mice. Unlike sHCC74 and SAR-HCV, both sHCC29 and sHCC63 had no detectable surface HLA class I antigen expression, alongside undetected intracellular β (2)-microglobulin (β (2)m) and marked HLA class I heavy chain and selective antigen-processing machinery (APM) component downregulation. The loss of β (2)m in sHCC29 and sHCC63 was caused by a >49 kb deletion across the B2M locus, while their downregulation of APM components was transcriptional, reversible by IFN-γ only in several components. β (2)m was also undetected in the primary HCC lesions of the patients involved, indicating its in vivo relevance. We report for the first time HLA class I antigen loss with underlying B2M gene deficiency and APM defects in 50% (2 of 4) of the sHCC cell lines tested. These findings may have implications for a proper design of T cell immunotherapy for the treatment of sHCC patients. Hindawi 2018-12-16 /pmc/articles/PMC6311956/ /pubmed/30648121 http://dx.doi.org/10.1155/2018/8363265 Text en Copyright © 2018 Wei-Yi Lei et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Lei, Wei-Yi Hsiung, Shih-Chieh Wen, Shao-Hsuan Hsieh, Chin-Hsuan Chen, Chien-Lin Wallace, Christopher Glenn Chang, Chien-Chung Liao, Shuen-Kuei Total HLA Class I Antigen Loss with the Downregulation of Antigen-Processing Machinery Components in Two Newly Established Sarcomatoid Hepatocellular Carcinoma Cell Lines |
title | Total HLA Class I Antigen Loss with the Downregulation of Antigen-Processing Machinery Components in Two Newly Established Sarcomatoid Hepatocellular Carcinoma Cell Lines |
title_full | Total HLA Class I Antigen Loss with the Downregulation of Antigen-Processing Machinery Components in Two Newly Established Sarcomatoid Hepatocellular Carcinoma Cell Lines |
title_fullStr | Total HLA Class I Antigen Loss with the Downregulation of Antigen-Processing Machinery Components in Two Newly Established Sarcomatoid Hepatocellular Carcinoma Cell Lines |
title_full_unstemmed | Total HLA Class I Antigen Loss with the Downregulation of Antigen-Processing Machinery Components in Two Newly Established Sarcomatoid Hepatocellular Carcinoma Cell Lines |
title_short | Total HLA Class I Antigen Loss with the Downregulation of Antigen-Processing Machinery Components in Two Newly Established Sarcomatoid Hepatocellular Carcinoma Cell Lines |
title_sort | total hla class i antigen loss with the downregulation of antigen-processing machinery components in two newly established sarcomatoid hepatocellular carcinoma cell lines |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6311956/ https://www.ncbi.nlm.nih.gov/pubmed/30648121 http://dx.doi.org/10.1155/2018/8363265 |
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