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qPCR detection of Sturgeon chub (Macrhybopsis gelida) DNA in environmental samples
The Sturgeon chub (Macrhybopsis gelida) is a cyprinid fish native to the Missouri and Mississippi River basins of the U.S. Suspected long-term declines in the size of its distribution have prompted a review of its conservation status by the U.S. Fish and Wildlife Service, a process which depends on...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6312230/ https://www.ncbi.nlm.nih.gov/pubmed/30596729 http://dx.doi.org/10.1371/journal.pone.0209601 |
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author | Mason, Daniel H. Dysthe, Joseph C. Franklin, Thomas W. Skorupski, Joseph A. Young, Michael K. McKelvey, Kevin S. Schwartz, Michael K. |
author_facet | Mason, Daniel H. Dysthe, Joseph C. Franklin, Thomas W. Skorupski, Joseph A. Young, Michael K. McKelvey, Kevin S. Schwartz, Michael K. |
author_sort | Mason, Daniel H. |
collection | PubMed |
description | The Sturgeon chub (Macrhybopsis gelida) is a cyprinid fish native to the Missouri and Mississippi River basins of the U.S. Suspected long-term declines in the size of its distribution have prompted a review of its conservation status by the U.S. Fish and Wildlife Service, a process which depends on reliable methods to delineate the distribution and status of extant populations. To facilitate monitoring of Sturgeon chub populations, we developed a quantitative PCR assay to detect Sturgeon chub DNA in environmental samples. The assay consistently detected Sturgeon chub DNA in concentrations as low as 2 copies per reaction, and did not amplify DNA from non-target fish species that are sympatric in the upper Missouri River basin. Field tests of this assay with environmental samples successfully detected Sturgeon chub from sites known to be occupied. This assay offers an extremely sensitive methodology that can be applied to determine the range of Sturgeon chub, regardless of variation in habitat characteristics. |
format | Online Article Text |
id | pubmed-6312230 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-63122302019-01-08 qPCR detection of Sturgeon chub (Macrhybopsis gelida) DNA in environmental samples Mason, Daniel H. Dysthe, Joseph C. Franklin, Thomas W. Skorupski, Joseph A. Young, Michael K. McKelvey, Kevin S. Schwartz, Michael K. PLoS One Research Article The Sturgeon chub (Macrhybopsis gelida) is a cyprinid fish native to the Missouri and Mississippi River basins of the U.S. Suspected long-term declines in the size of its distribution have prompted a review of its conservation status by the U.S. Fish and Wildlife Service, a process which depends on reliable methods to delineate the distribution and status of extant populations. To facilitate monitoring of Sturgeon chub populations, we developed a quantitative PCR assay to detect Sturgeon chub DNA in environmental samples. The assay consistently detected Sturgeon chub DNA in concentrations as low as 2 copies per reaction, and did not amplify DNA from non-target fish species that are sympatric in the upper Missouri River basin. Field tests of this assay with environmental samples successfully detected Sturgeon chub from sites known to be occupied. This assay offers an extremely sensitive methodology that can be applied to determine the range of Sturgeon chub, regardless of variation in habitat characteristics. Public Library of Science 2018-12-31 /pmc/articles/PMC6312230/ /pubmed/30596729 http://dx.doi.org/10.1371/journal.pone.0209601 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication. |
spellingShingle | Research Article Mason, Daniel H. Dysthe, Joseph C. Franklin, Thomas W. Skorupski, Joseph A. Young, Michael K. McKelvey, Kevin S. Schwartz, Michael K. qPCR detection of Sturgeon chub (Macrhybopsis gelida) DNA in environmental samples |
title | qPCR detection of Sturgeon chub (Macrhybopsis gelida) DNA in environmental samples |
title_full | qPCR detection of Sturgeon chub (Macrhybopsis gelida) DNA in environmental samples |
title_fullStr | qPCR detection of Sturgeon chub (Macrhybopsis gelida) DNA in environmental samples |
title_full_unstemmed | qPCR detection of Sturgeon chub (Macrhybopsis gelida) DNA in environmental samples |
title_short | qPCR detection of Sturgeon chub (Macrhybopsis gelida) DNA in environmental samples |
title_sort | qpcr detection of sturgeon chub (macrhybopsis gelida) dna in environmental samples |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6312230/ https://www.ncbi.nlm.nih.gov/pubmed/30596729 http://dx.doi.org/10.1371/journal.pone.0209601 |
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