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Can stable isotope markers be used to distinguish wild and mass-reared Anastrepha fraterculus flies?
The availability of accurate techniques to discriminate between marked laboratory-reared flies and unmarked wild flies captured in monitoring traps is essential for programs that integrate the Sterile Insect Technique (SIT) to manage fruit flies. In this study, the feasibility of using a stable isot...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6312238/ https://www.ncbi.nlm.nih.gov/pubmed/30596760 http://dx.doi.org/10.1371/journal.pone.0209921 |
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author | Botteon, Victor Costa, Maria de Lourdes Zamboni Kovaleski, Adalecio Martinelli, Luiz Antonio Mastrangelo, Thiago |
author_facet | Botteon, Victor Costa, Maria de Lourdes Zamboni Kovaleski, Adalecio Martinelli, Luiz Antonio Mastrangelo, Thiago |
author_sort | Botteon, Victor |
collection | PubMed |
description | The availability of accurate techniques to discriminate between marked laboratory-reared flies and unmarked wild flies captured in monitoring traps is essential for programs that integrate the Sterile Insect Technique (SIT) to manage fruit flies. In this study, the feasibility of using a stable isotope marking technique for the South American fruit fly, Anastrepha fraterculus (Wiedemann), was assessed. Wild flies were collected from apple orchards, which are a target of a SIT project in southern Brazil. To verify if adult flies could be labelled by the stable isotopes from larval diets, larvae were reared on two different C(4)-based diets and fruits in laboratory. To evaluate the influence of the two most common attractants applied to capture A. fraterculus (grape juice and CeraTrap(TM)) and the most common preservation method in fruit fly collections (ethanol), laboratory-reared flies were immersed in McPhail traps containing the respective treatments for two periods of time. Samples were analyzed in an elemental analyzer coupled to a Continuous Flow Isotope Ratio Mass Spectrometer (CF-IRMS) at CENA/USP. The δ(13)C signatures of flies reared on artificial diets differed significantly from the δ(13)C of flies whose larvae were reared on fruits and from wild flies. In contrast, the δ(15)N values were less conclusive and the technique could not rely solely on them. In all cases considered, the δ(13)C and δ(15)N signatures from males did not differ from females. Despite the alterations caused by the attractants tested and ethanol, laboratory-flies could be distinguished from the wild ones based on δ(13)C signatures. This is the first comprehensive study to demonstrate that it is possible to distinguish wild A. fraterculus from flies reared on larval diets containing C(4) sugar. The first experimentally derived trophic discrimination factors were also obtained for this species. Thus, intrinsic isotope labelling can serve as a backup to conventional dye marking. |
format | Online Article Text |
id | pubmed-6312238 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-63122382019-01-08 Can stable isotope markers be used to distinguish wild and mass-reared Anastrepha fraterculus flies? Botteon, Victor Costa, Maria de Lourdes Zamboni Kovaleski, Adalecio Martinelli, Luiz Antonio Mastrangelo, Thiago PLoS One Research Article The availability of accurate techniques to discriminate between marked laboratory-reared flies and unmarked wild flies captured in monitoring traps is essential for programs that integrate the Sterile Insect Technique (SIT) to manage fruit flies. In this study, the feasibility of using a stable isotope marking technique for the South American fruit fly, Anastrepha fraterculus (Wiedemann), was assessed. Wild flies were collected from apple orchards, which are a target of a SIT project in southern Brazil. To verify if adult flies could be labelled by the stable isotopes from larval diets, larvae were reared on two different C(4)-based diets and fruits in laboratory. To evaluate the influence of the two most common attractants applied to capture A. fraterculus (grape juice and CeraTrap(TM)) and the most common preservation method in fruit fly collections (ethanol), laboratory-reared flies were immersed in McPhail traps containing the respective treatments for two periods of time. Samples were analyzed in an elemental analyzer coupled to a Continuous Flow Isotope Ratio Mass Spectrometer (CF-IRMS) at CENA/USP. The δ(13)C signatures of flies reared on artificial diets differed significantly from the δ(13)C of flies whose larvae were reared on fruits and from wild flies. In contrast, the δ(15)N values were less conclusive and the technique could not rely solely on them. In all cases considered, the δ(13)C and δ(15)N signatures from males did not differ from females. Despite the alterations caused by the attractants tested and ethanol, laboratory-flies could be distinguished from the wild ones based on δ(13)C signatures. This is the first comprehensive study to demonstrate that it is possible to distinguish wild A. fraterculus from flies reared on larval diets containing C(4) sugar. The first experimentally derived trophic discrimination factors were also obtained for this species. Thus, intrinsic isotope labelling can serve as a backup to conventional dye marking. Public Library of Science 2018-12-31 /pmc/articles/PMC6312238/ /pubmed/30596760 http://dx.doi.org/10.1371/journal.pone.0209921 Text en © 2018 Botteon et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Botteon, Victor Costa, Maria de Lourdes Zamboni Kovaleski, Adalecio Martinelli, Luiz Antonio Mastrangelo, Thiago Can stable isotope markers be used to distinguish wild and mass-reared Anastrepha fraterculus flies? |
title | Can stable isotope markers be used to distinguish wild and mass-reared Anastrepha fraterculus flies? |
title_full | Can stable isotope markers be used to distinguish wild and mass-reared Anastrepha fraterculus flies? |
title_fullStr | Can stable isotope markers be used to distinguish wild and mass-reared Anastrepha fraterculus flies? |
title_full_unstemmed | Can stable isotope markers be used to distinguish wild and mass-reared Anastrepha fraterculus flies? |
title_short | Can stable isotope markers be used to distinguish wild and mass-reared Anastrepha fraterculus flies? |
title_sort | can stable isotope markers be used to distinguish wild and mass-reared anastrepha fraterculus flies? |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6312238/ https://www.ncbi.nlm.nih.gov/pubmed/30596760 http://dx.doi.org/10.1371/journal.pone.0209921 |
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