Cargando…
High-resolution magic angle spinning NMR studies for metabolic characterization of Arabidopsis thaliana mutants with enhanced growth characteristics
Developing smart crops which yield more biomass to meet the increasing demand for plant biomass has been an active area of research in last few decades. We investigated metabolic alterations in two Arabidopsis thaliana mutants with enhanced growth characteristics that were previously obtained from a...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6312362/ https://www.ncbi.nlm.nih.gov/pubmed/30596736 http://dx.doi.org/10.1371/journal.pone.0209695 |
_version_ | 1783383770752090112 |
---|---|
author | Augustijn, Dieuwertje van Tol, Niels van der Zaal, Bert J. de Groot, Huub J. M. Alia, A. |
author_facet | Augustijn, Dieuwertje van Tol, Niels van der Zaal, Bert J. de Groot, Huub J. M. Alia, A. |
author_sort | Augustijn, Dieuwertje |
collection | PubMed |
description | Developing smart crops which yield more biomass to meet the increasing demand for plant biomass has been an active area of research in last few decades. We investigated metabolic alterations in two Arabidopsis thaliana mutants with enhanced growth characteristics that were previously obtained from a collection of plant lines expressing artificial transcription factors. The metabolic profiles were obtained directly from intact Arabidopsis leaves using high-resolution magic angle spinning (HR-MAS) NMR. Multivariate analysis showed significant alteration of metabolite levels between the mutants and the wild-type Col-0. Interestingly, most of the metabolites that were reduced in the faster-growing mutants are generally involved in the defence against stress. These results suggest a growth-defence trade-off in the phenotypically engineered mutants. Our results further corroborate the idea that plant growth can be enhanced by suppressing defence pathways. |
format | Online Article Text |
id | pubmed-6312362 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-63123622019-01-08 High-resolution magic angle spinning NMR studies for metabolic characterization of Arabidopsis thaliana mutants with enhanced growth characteristics Augustijn, Dieuwertje van Tol, Niels van der Zaal, Bert J. de Groot, Huub J. M. Alia, A. PLoS One Research Article Developing smart crops which yield more biomass to meet the increasing demand for plant biomass has been an active area of research in last few decades. We investigated metabolic alterations in two Arabidopsis thaliana mutants with enhanced growth characteristics that were previously obtained from a collection of plant lines expressing artificial transcription factors. The metabolic profiles were obtained directly from intact Arabidopsis leaves using high-resolution magic angle spinning (HR-MAS) NMR. Multivariate analysis showed significant alteration of metabolite levels between the mutants and the wild-type Col-0. Interestingly, most of the metabolites that were reduced in the faster-growing mutants are generally involved in the defence against stress. These results suggest a growth-defence trade-off in the phenotypically engineered mutants. Our results further corroborate the idea that plant growth can be enhanced by suppressing defence pathways. Public Library of Science 2018-12-31 /pmc/articles/PMC6312362/ /pubmed/30596736 http://dx.doi.org/10.1371/journal.pone.0209695 Text en © 2018 Augustijn et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Augustijn, Dieuwertje van Tol, Niels van der Zaal, Bert J. de Groot, Huub J. M. Alia, A. High-resolution magic angle spinning NMR studies for metabolic characterization of Arabidopsis thaliana mutants with enhanced growth characteristics |
title | High-resolution magic angle spinning NMR studies for metabolic characterization of Arabidopsis thaliana mutants with enhanced growth characteristics |
title_full | High-resolution magic angle spinning NMR studies for metabolic characterization of Arabidopsis thaliana mutants with enhanced growth characteristics |
title_fullStr | High-resolution magic angle spinning NMR studies for metabolic characterization of Arabidopsis thaliana mutants with enhanced growth characteristics |
title_full_unstemmed | High-resolution magic angle spinning NMR studies for metabolic characterization of Arabidopsis thaliana mutants with enhanced growth characteristics |
title_short | High-resolution magic angle spinning NMR studies for metabolic characterization of Arabidopsis thaliana mutants with enhanced growth characteristics |
title_sort | high-resolution magic angle spinning nmr studies for metabolic characterization of arabidopsis thaliana mutants with enhanced growth characteristics |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6312362/ https://www.ncbi.nlm.nih.gov/pubmed/30596736 http://dx.doi.org/10.1371/journal.pone.0209695 |
work_keys_str_mv | AT augustijndieuwertje highresolutionmagicanglespinningnmrstudiesformetaboliccharacterizationofarabidopsisthalianamutantswithenhancedgrowthcharacteristics AT vantolniels highresolutionmagicanglespinningnmrstudiesformetaboliccharacterizationofarabidopsisthalianamutantswithenhancedgrowthcharacteristics AT vanderzaalbertj highresolutionmagicanglespinningnmrstudiesformetaboliccharacterizationofarabidopsisthalianamutantswithenhancedgrowthcharacteristics AT degroothuubjm highresolutionmagicanglespinningnmrstudiesformetaboliccharacterizationofarabidopsisthalianamutantswithenhancedgrowthcharacteristics AT aliaa highresolutionmagicanglespinningnmrstudiesformetaboliccharacterizationofarabidopsisthalianamutantswithenhancedgrowthcharacteristics |