Construction and next-generation sequencing analysis of a large phage-displayed V(NAR) single-domain antibody library from six naïve nurse sharks

BACKGROUND: Shark new antigen receptor variable domain (V(NAR)) antibodies can bind restricted epitopes that may be inaccessible to conventional antibodies. METHODS: Here, we developed a library construction method based on polymerase chain reaction (PCR)-Extension Assembly and Self-Ligation (named...

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Detalles Bibliográficos
Autores principales: Feng, Mingqian, Bian, Hejiao, Wu, Xiaolin, Fu, Tianyun, Fu, Ying, Hong, Jessica, Fleming, Bryan D, Flajnik, Martin F, Ho, Mitchell
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6312525/
https://www.ncbi.nlm.nih.gov/pubmed/30627698
http://dx.doi.org/10.1093/abt/tby011

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