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Genotype-specific suppression of multiple defense pathways in apple root during infection by Pythium ultimum
The genotype-specific defense activation in the roots of perennial tree crops to soilborne necrotrophic pathogens remains largely unknown. A recent phenotyping study indicated that the apple rootstock genotypes B.9 and G.935 have contrasting resistance responses to infection by Pythium ultimum. In t...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6312547/ https://www.ncbi.nlm.nih.gov/pubmed/30603095 http://dx.doi.org/10.1038/s41438-018-0087-1 |
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author | Zhu, Yanmin Shao, Jonathan Zhou, Zhe Davis, Robert E. |
author_facet | Zhu, Yanmin Shao, Jonathan Zhou, Zhe Davis, Robert E. |
author_sort | Zhu, Yanmin |
collection | PubMed |
description | The genotype-specific defense activation in the roots of perennial tree crops to soilborne necrotrophic pathogens remains largely unknown. A recent phenotyping study indicated that the apple rootstock genotypes B.9 and G.935 have contrasting resistance responses to infection by Pythium ultimum. In the current study, a comparative transcriptome analysis by Illumina Solexa HiSeq 3000 platform was carried out to identify the global transcriptional regulation networks between the susceptible B.9 and the resistant G.935 to P. ultimum infection. Thirty-six libraries were sequenced to cover three timepoints after pathogen inoculation, with three biological replicates for each sample. The transcriptomes in the roots of the susceptible genotype B.9 were reflected by overrepresented differentially expressed genes (DEGs) with downregulated patterns and systematic suppression of cellular processes at 48 h post inoculation (hpi). In contrast, DEGs with annotated functions, such as kinase receptors, MAPK signaling, JA biosynthesis enzymes, transcription factors, and transporters, were readily induced at 24 hpi and continued up-regulation at 48 hpi in G.935 roots. The earlier and stronger defense activation is likely associated with an effective inhibition of necrosis progression in G.935 roots. Lack of effector-triggered immunity or existence of a susceptibility gene could contribute to the severely disturbed transcriptome and susceptibility in B.9 roots. The identified DEGs constitute a valuable resource for hypothesis-driven studies to elucidate the resistance/tolerance mechanisms in apple roots and validating their potential association with resistance traits. |
format | Online Article Text |
id | pubmed-6312547 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-63125472019-01-02 Genotype-specific suppression of multiple defense pathways in apple root during infection by Pythium ultimum Zhu, Yanmin Shao, Jonathan Zhou, Zhe Davis, Robert E. Hortic Res Article The genotype-specific defense activation in the roots of perennial tree crops to soilborne necrotrophic pathogens remains largely unknown. A recent phenotyping study indicated that the apple rootstock genotypes B.9 and G.935 have contrasting resistance responses to infection by Pythium ultimum. In the current study, a comparative transcriptome analysis by Illumina Solexa HiSeq 3000 platform was carried out to identify the global transcriptional regulation networks between the susceptible B.9 and the resistant G.935 to P. ultimum infection. Thirty-six libraries were sequenced to cover three timepoints after pathogen inoculation, with three biological replicates for each sample. The transcriptomes in the roots of the susceptible genotype B.9 were reflected by overrepresented differentially expressed genes (DEGs) with downregulated patterns and systematic suppression of cellular processes at 48 h post inoculation (hpi). In contrast, DEGs with annotated functions, such as kinase receptors, MAPK signaling, JA biosynthesis enzymes, transcription factors, and transporters, were readily induced at 24 hpi and continued up-regulation at 48 hpi in G.935 roots. The earlier and stronger defense activation is likely associated with an effective inhibition of necrosis progression in G.935 roots. Lack of effector-triggered immunity or existence of a susceptibility gene could contribute to the severely disturbed transcriptome and susceptibility in B.9 roots. The identified DEGs constitute a valuable resource for hypothesis-driven studies to elucidate the resistance/tolerance mechanisms in apple roots and validating their potential association with resistance traits. Nature Publishing Group UK 2019-01-01 /pmc/articles/PMC6312547/ /pubmed/30603095 http://dx.doi.org/10.1038/s41438-018-0087-1 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Zhu, Yanmin Shao, Jonathan Zhou, Zhe Davis, Robert E. Genotype-specific suppression of multiple defense pathways in apple root during infection by Pythium ultimum |
title | Genotype-specific suppression of multiple defense pathways in apple root during infection by Pythium ultimum |
title_full | Genotype-specific suppression of multiple defense pathways in apple root during infection by Pythium ultimum |
title_fullStr | Genotype-specific suppression of multiple defense pathways in apple root during infection by Pythium ultimum |
title_full_unstemmed | Genotype-specific suppression of multiple defense pathways in apple root during infection by Pythium ultimum |
title_short | Genotype-specific suppression of multiple defense pathways in apple root during infection by Pythium ultimum |
title_sort | genotype-specific suppression of multiple defense pathways in apple root during infection by pythium ultimum |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6312547/ https://www.ncbi.nlm.nih.gov/pubmed/30603095 http://dx.doi.org/10.1038/s41438-018-0087-1 |
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