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The interaction of CpEBF1 with CpMADSs is involved in cell wall degradation during papaya fruit ripening

Ethylene plays a pivotal role in climacteric fruit ripening; whereas 1-MCP, a non-toxic antagonist of ethylene, prevents ethylene-dependent responses and fruit ripening. In this study, a short-term treatment (1 h) with 400 nL L(−1) 1-MCP delayed the ripening of harvested papaya. However, long-term a...

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Autores principales: Ding, Xiaochun, Zhu, Xiaoyang, Ye, Lanlan, Xiao, Shuangling, Wu, Zhenxian, Chen, Weixin, Li, Xueping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6312555/
https://www.ncbi.nlm.nih.gov/pubmed/30622723
http://dx.doi.org/10.1038/s41438-018-0095-1
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author Ding, Xiaochun
Zhu, Xiaoyang
Ye, Lanlan
Xiao, Shuangling
Wu, Zhenxian
Chen, Weixin
Li, Xueping
author_facet Ding, Xiaochun
Zhu, Xiaoyang
Ye, Lanlan
Xiao, Shuangling
Wu, Zhenxian
Chen, Weixin
Li, Xueping
author_sort Ding, Xiaochun
collection PubMed
description Ethylene plays a pivotal role in climacteric fruit ripening; whereas 1-MCP, a non-toxic antagonist of ethylene, prevents ethylene-dependent responses and fruit ripening. In this study, a short-term treatment (1 h) with 400 nL L(−1) 1-MCP delayed the ripening of harvested papaya. However, long-term application of 1-MCP (400 nL L(−1), 16 h) resulted in abnormal fruit ripening, with the fruits exhibiting normal yellowing without softening, significantly higher cellulose and lignin contents, and intact cell walls (CW). Furthermore, we found that long-term treatment with 1-MCP significantly inhibited the expression of CpEBF1, an EIN3-binding F-box-1 gene. A protein interaction analysis using yeast two-hybrid, BiFC and GST pull-down assays showed that CpEBF1 interacts with the CpMADS1/3 and CpEIL1 proteins. The interaction of CpEBF1 with CpMADS1/3 further activated the activities of CW-degradation gene promoters. Subcellular localization showed that these proteins were localized in the nucleus. Additionally, the expression levels of CpMADS1/3, CpEIL1, and several CW-degradation-related genes were significantly downregulated by long-term 1-MCP treatment. Therefore, we propose that the inhibited expression of CpEBF1 and CpMADS1/3 resulted in the repressed activation of CW-degradation-related genes via their interaction, thereby resulting in fruit softening disorders.
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spelling pubmed-63125552019-01-08 The interaction of CpEBF1 with CpMADSs is involved in cell wall degradation during papaya fruit ripening Ding, Xiaochun Zhu, Xiaoyang Ye, Lanlan Xiao, Shuangling Wu, Zhenxian Chen, Weixin Li, Xueping Hortic Res Article Ethylene plays a pivotal role in climacteric fruit ripening; whereas 1-MCP, a non-toxic antagonist of ethylene, prevents ethylene-dependent responses and fruit ripening. In this study, a short-term treatment (1 h) with 400 nL L(−1) 1-MCP delayed the ripening of harvested papaya. However, long-term application of 1-MCP (400 nL L(−1), 16 h) resulted in abnormal fruit ripening, with the fruits exhibiting normal yellowing without softening, significantly higher cellulose and lignin contents, and intact cell walls (CW). Furthermore, we found that long-term treatment with 1-MCP significantly inhibited the expression of CpEBF1, an EIN3-binding F-box-1 gene. A protein interaction analysis using yeast two-hybrid, BiFC and GST pull-down assays showed that CpEBF1 interacts with the CpMADS1/3 and CpEIL1 proteins. The interaction of CpEBF1 with CpMADS1/3 further activated the activities of CW-degradation gene promoters. Subcellular localization showed that these proteins were localized in the nucleus. Additionally, the expression levels of CpMADS1/3, CpEIL1, and several CW-degradation-related genes were significantly downregulated by long-term 1-MCP treatment. Therefore, we propose that the inhibited expression of CpEBF1 and CpMADS1/3 resulted in the repressed activation of CW-degradation-related genes via their interaction, thereby resulting in fruit softening disorders. Nature Publishing Group UK 2019-01-01 /pmc/articles/PMC6312555/ /pubmed/30622723 http://dx.doi.org/10.1038/s41438-018-0095-1 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Ding, Xiaochun
Zhu, Xiaoyang
Ye, Lanlan
Xiao, Shuangling
Wu, Zhenxian
Chen, Weixin
Li, Xueping
The interaction of CpEBF1 with CpMADSs is involved in cell wall degradation during papaya fruit ripening
title The interaction of CpEBF1 with CpMADSs is involved in cell wall degradation during papaya fruit ripening
title_full The interaction of CpEBF1 with CpMADSs is involved in cell wall degradation during papaya fruit ripening
title_fullStr The interaction of CpEBF1 with CpMADSs is involved in cell wall degradation during papaya fruit ripening
title_full_unstemmed The interaction of CpEBF1 with CpMADSs is involved in cell wall degradation during papaya fruit ripening
title_short The interaction of CpEBF1 with CpMADSs is involved in cell wall degradation during papaya fruit ripening
title_sort interaction of cpebf1 with cpmadss is involved in cell wall degradation during papaya fruit ripening
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6312555/
https://www.ncbi.nlm.nih.gov/pubmed/30622723
http://dx.doi.org/10.1038/s41438-018-0095-1
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