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Abundant Small Genetic Alterations after Upland Cotton Domestication

Domestication has long been recognized as the most direct and effective way to intentionally influence morphological and physiological phenotypes in plants and animals. Consequently, understanding how small genetic alterations contribute to domestication is of considerable importance. In this study,...

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Autores principales: Bao, Ying, Zhang, Xia, Xu, Xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6312614/
https://www.ncbi.nlm.nih.gov/pubmed/30631774
http://dx.doi.org/10.1155/2018/9254302
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author Bao, Ying
Zhang, Xia
Xu, Xin
author_facet Bao, Ying
Zhang, Xia
Xu, Xin
author_sort Bao, Ying
collection PubMed
description Domestication has long been recognized as the most direct and effective way to intentionally influence morphological and physiological phenotypes in plants and animals. Consequently, understanding how small genetic alterations contribute to domestication is of considerable importance. In this study, we resequenced the genome of the wild upland cotton variety Gossypium hirsutum var. yucatanense, the putative wild ancestor of cultivated upland cotton, and then compared single nucleotide polymorphism (SNP) and short insertion and deletion (InDel) variations of the genome with the cultivated accession (TM-1) of G. hirsutum. We found approximately 6.6 million SNPs and 0.7 million InDels between the two genomes. Most of the small genetic variations were anchored in the noncoding regions. With regard to potential coding genes, we found 24,035 genes with nonsynonymous SNPs. Interestingly, 2603 genes in domesticated cotton are found that have changed the positions of stop codons or shifted reading frames from that in G. hirsutum var. yucatanense. This suggests that domestication may have been selected for mutations that restored gene function or that wild cotton has undergone a number of gene inactivation events since its divergence from cultivated cotton. The former scenario seems most likely due to the intense selective pressure applied during the domestication process. These results demonstrate that, within a relatively short period of time, the cotton genome has been readjusted through small genetic changes. The current study provides useful clues for seeking interesting genes for cotton improvement.
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spelling pubmed-63126142019-01-10 Abundant Small Genetic Alterations after Upland Cotton Domestication Bao, Ying Zhang, Xia Xu, Xin Biomed Res Int Research Article Domestication has long been recognized as the most direct and effective way to intentionally influence morphological and physiological phenotypes in plants and animals. Consequently, understanding how small genetic alterations contribute to domestication is of considerable importance. In this study, we resequenced the genome of the wild upland cotton variety Gossypium hirsutum var. yucatanense, the putative wild ancestor of cultivated upland cotton, and then compared single nucleotide polymorphism (SNP) and short insertion and deletion (InDel) variations of the genome with the cultivated accession (TM-1) of G. hirsutum. We found approximately 6.6 million SNPs and 0.7 million InDels between the two genomes. Most of the small genetic variations were anchored in the noncoding regions. With regard to potential coding genes, we found 24,035 genes with nonsynonymous SNPs. Interestingly, 2603 genes in domesticated cotton are found that have changed the positions of stop codons or shifted reading frames from that in G. hirsutum var. yucatanense. This suggests that domestication may have been selected for mutations that restored gene function or that wild cotton has undergone a number of gene inactivation events since its divergence from cultivated cotton. The former scenario seems most likely due to the intense selective pressure applied during the domestication process. These results demonstrate that, within a relatively short period of time, the cotton genome has been readjusted through small genetic changes. The current study provides useful clues for seeking interesting genes for cotton improvement. Hindawi 2018-12-18 /pmc/articles/PMC6312614/ /pubmed/30631774 http://dx.doi.org/10.1155/2018/9254302 Text en Copyright © 2018 Ying Bao et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Bao, Ying
Zhang, Xia
Xu, Xin
Abundant Small Genetic Alterations after Upland Cotton Domestication
title Abundant Small Genetic Alterations after Upland Cotton Domestication
title_full Abundant Small Genetic Alterations after Upland Cotton Domestication
title_fullStr Abundant Small Genetic Alterations after Upland Cotton Domestication
title_full_unstemmed Abundant Small Genetic Alterations after Upland Cotton Domestication
title_short Abundant Small Genetic Alterations after Upland Cotton Domestication
title_sort abundant small genetic alterations after upland cotton domestication
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6312614/
https://www.ncbi.nlm.nih.gov/pubmed/30631774
http://dx.doi.org/10.1155/2018/9254302
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