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Potential therapeutic effect of pomegranate seed oil on ovarian ischemia/reperfusion injury in rats

OBJECTIVE(S): The aim of this study is to determine the therapeutic effects of pomegranate seed oil, which is a powerful antioxidant and anti-inflammatory agent, on ovarian-ischemia and reperfusion injury in rats. MATERIALS AND METHODS: Fifty-six female albino Wistar rats were divided into 7 equal g...

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Autores principales: Yayla, Muhammed, Cetin, Damla, Adali, Yasemen, Kilicle, Pinar Aksu, Toktay, Erdem
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6312678/
https://www.ncbi.nlm.nih.gov/pubmed/30627370
http://dx.doi.org/10.22038/ijbms.2018.30149.7268
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author Yayla, Muhammed
Cetin, Damla
Adali, Yasemen
Kilicle, Pinar Aksu
Toktay, Erdem
author_facet Yayla, Muhammed
Cetin, Damla
Adali, Yasemen
Kilicle, Pinar Aksu
Toktay, Erdem
author_sort Yayla, Muhammed
collection PubMed
description OBJECTIVE(S): The aim of this study is to determine the therapeutic effects of pomegranate seed oil, which is a powerful antioxidant and anti-inflammatory agent, on ovarian-ischemia and reperfusion injury in rats. MATERIALS AND METHODS: Fifty-six female albino Wistar rats were divided into 7 equal groups. Group 1; Sham Operation, Group 2; Ischemia, Group 3; Ischemia + Reperfusion, Group 4; Ischemia + Pomegranate 0,32 ml / kg (IP), Group 5; Ischemia + Pomegranate 0.64 ml / kg, Group 6; Ischemia + Pomegranate 0,32 ml / kg + reperfusion, Group 7; Ischemia + Pomegranate 0,64 ml / kg + reperfusion. Three hours after ischemia and 3 hours after reperfusion, the study was terminated. RESULTS: While NADPH oxidase activity, MDA and TNF-α levels were significantly increased, SOD activity and GSH levels were reduced in ischemia and I/R groups. Low dose pomegranate seed oil application reduced significantly oxidative stress and NADPH oxidase activity in both ischemic and ischemic/reperfusion groups. At the same time, low-dose pomegranate seed oil extract reduced TNF-α levels and significantly increased antioxidant activity. CONCLUSION: PSO demonstrated an important therapeutic effect in the treatment of ovarian ischemia and reperfusion injury.
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spelling pubmed-63126782019-01-09 Potential therapeutic effect of pomegranate seed oil on ovarian ischemia/reperfusion injury in rats Yayla, Muhammed Cetin, Damla Adali, Yasemen Kilicle, Pinar Aksu Toktay, Erdem Iran J Basic Med Sci Original Article OBJECTIVE(S): The aim of this study is to determine the therapeutic effects of pomegranate seed oil, which is a powerful antioxidant and anti-inflammatory agent, on ovarian-ischemia and reperfusion injury in rats. MATERIALS AND METHODS: Fifty-six female albino Wistar rats were divided into 7 equal groups. Group 1; Sham Operation, Group 2; Ischemia, Group 3; Ischemia + Reperfusion, Group 4; Ischemia + Pomegranate 0,32 ml / kg (IP), Group 5; Ischemia + Pomegranate 0.64 ml / kg, Group 6; Ischemia + Pomegranate 0,32 ml / kg + reperfusion, Group 7; Ischemia + Pomegranate 0,64 ml / kg + reperfusion. Three hours after ischemia and 3 hours after reperfusion, the study was terminated. RESULTS: While NADPH oxidase activity, MDA and TNF-α levels were significantly increased, SOD activity and GSH levels were reduced in ischemia and I/R groups. Low dose pomegranate seed oil application reduced significantly oxidative stress and NADPH oxidase activity in both ischemic and ischemic/reperfusion groups. At the same time, low-dose pomegranate seed oil extract reduced TNF-α levels and significantly increased antioxidant activity. CONCLUSION: PSO demonstrated an important therapeutic effect in the treatment of ovarian ischemia and reperfusion injury. Mashhad University of Medical Sciences 2018-12 /pmc/articles/PMC6312678/ /pubmed/30627370 http://dx.doi.org/10.22038/ijbms.2018.30149.7268 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Yayla, Muhammed
Cetin, Damla
Adali, Yasemen
Kilicle, Pinar Aksu
Toktay, Erdem
Potential therapeutic effect of pomegranate seed oil on ovarian ischemia/reperfusion injury in rats
title Potential therapeutic effect of pomegranate seed oil on ovarian ischemia/reperfusion injury in rats
title_full Potential therapeutic effect of pomegranate seed oil on ovarian ischemia/reperfusion injury in rats
title_fullStr Potential therapeutic effect of pomegranate seed oil on ovarian ischemia/reperfusion injury in rats
title_full_unstemmed Potential therapeutic effect of pomegranate seed oil on ovarian ischemia/reperfusion injury in rats
title_short Potential therapeutic effect of pomegranate seed oil on ovarian ischemia/reperfusion injury in rats
title_sort potential therapeutic effect of pomegranate seed oil on ovarian ischemia/reperfusion injury in rats
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6312678/
https://www.ncbi.nlm.nih.gov/pubmed/30627370
http://dx.doi.org/10.22038/ijbms.2018.30149.7268
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