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Genome‐wide analysis of long non‐coding RNAs in esophageal squamous cell carcinoma reveals their potential role in invasion and metastasis

BACKGROUND: A high lymphatic metastasis rate and strong local invasive ability are the key characteristics of esophageal squamous cell carcinoma (ESCC) that affect patient survival, and long non‐coding RNAs (lncRNAs) may play a crucial role. We performed genome‐wide analysis of lncRNAs to identify n...

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Autores principales: Wang, Jihua, Sun, Dianshui, Wu, Kai, Liu, Jie, Zhao, Miaoqing, Li, Xiaomei, Xu, Ying, Li, Baosheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons Australia, Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6312850/
https://www.ncbi.nlm.nih.gov/pubmed/30390388
http://dx.doi.org/10.1111/1759-7714.12904
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author Wang, Jihua
Sun, Dianshui
Wu, Kai
Liu, Jie
Zhao, Miaoqing
Li, Xiaomei
Xu, Ying
Li, Baosheng
author_facet Wang, Jihua
Sun, Dianshui
Wu, Kai
Liu, Jie
Zhao, Miaoqing
Li, Xiaomei
Xu, Ying
Li, Baosheng
author_sort Wang, Jihua
collection PubMed
description BACKGROUND: A high lymphatic metastasis rate and strong local invasive ability are the key characteristics of esophageal squamous cell carcinoma (ESCC) that affect patient survival, and long non‐coding RNAs (lncRNAs) may play a crucial role. We performed genome‐wide analysis of lncRNAs to identify novel biomarkers associated with local invasion and lymphatic metastasis in ESCC. METHODS: Six pairs of ESCC tumor and para‐tumor tissues were subjected to microarray analysis to identify differentially expressed lncRNAs, and 25 pairs of tissues samples were used to verify the effectiveness of screened lncRNAs using quantitative reverse transcription PCR. The correlations between verified lncRNAs and clinicopathological characteristics were analyzed to confirm specific lncRNAs associated with the local invasion and lymphatic metastasis of ESCC, and gene co‐expression analysis was used to predict potential mechanisms. RESULTS: Microarray analysis identified 1850 lncRNAs with significant differential expression in ESCC. Of 22 lncRNAs selected for quantitative reverse transcription PCR verification, four were significantly upregulated and one was significantly downregulated in ESCC cancer compared to para‐cancer tissues. ENST00000508406.1 was significantly associated with T, N, and tumor node metastasis stages, and NR_037652.1 was significantly associated with N stage. Moreover, 49 lncRNA‐messenger RNA pairs were significantly associated with the two dysregulated lncRNAs and possibly involved in the regulation of local invasion and lymphatic metastasis of ESCC. CONCLUSION: The present genome‐wide analysis identified two novel and tumor‐specific lncRNAs for predicting ESCC local invasion and lymphatic metastasis, providing insight into the potential underlying mechanism, which warrants further investigation.
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spelling pubmed-63128502019-01-07 Genome‐wide analysis of long non‐coding RNAs in esophageal squamous cell carcinoma reveals their potential role in invasion and metastasis Wang, Jihua Sun, Dianshui Wu, Kai Liu, Jie Zhao, Miaoqing Li, Xiaomei Xu, Ying Li, Baosheng Thorac Cancer Original Articles BACKGROUND: A high lymphatic metastasis rate and strong local invasive ability are the key characteristics of esophageal squamous cell carcinoma (ESCC) that affect patient survival, and long non‐coding RNAs (lncRNAs) may play a crucial role. We performed genome‐wide analysis of lncRNAs to identify novel biomarkers associated with local invasion and lymphatic metastasis in ESCC. METHODS: Six pairs of ESCC tumor and para‐tumor tissues were subjected to microarray analysis to identify differentially expressed lncRNAs, and 25 pairs of tissues samples were used to verify the effectiveness of screened lncRNAs using quantitative reverse transcription PCR. The correlations between verified lncRNAs and clinicopathological characteristics were analyzed to confirm specific lncRNAs associated with the local invasion and lymphatic metastasis of ESCC, and gene co‐expression analysis was used to predict potential mechanisms. RESULTS: Microarray analysis identified 1850 lncRNAs with significant differential expression in ESCC. Of 22 lncRNAs selected for quantitative reverse transcription PCR verification, four were significantly upregulated and one was significantly downregulated in ESCC cancer compared to para‐cancer tissues. ENST00000508406.1 was significantly associated with T, N, and tumor node metastasis stages, and NR_037652.1 was significantly associated with N stage. Moreover, 49 lncRNA‐messenger RNA pairs were significantly associated with the two dysregulated lncRNAs and possibly involved in the regulation of local invasion and lymphatic metastasis of ESCC. CONCLUSION: The present genome‐wide analysis identified two novel and tumor‐specific lncRNAs for predicting ESCC local invasion and lymphatic metastasis, providing insight into the potential underlying mechanism, which warrants further investigation. John Wiley & Sons Australia, Ltd 2018-11-03 2019-01 /pmc/articles/PMC6312850/ /pubmed/30390388 http://dx.doi.org/10.1111/1759-7714.12904 Text en © 2018 The Authors. Thoracic Cancer published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Original Articles
Wang, Jihua
Sun, Dianshui
Wu, Kai
Liu, Jie
Zhao, Miaoqing
Li, Xiaomei
Xu, Ying
Li, Baosheng
Genome‐wide analysis of long non‐coding RNAs in esophageal squamous cell carcinoma reveals their potential role in invasion and metastasis
title Genome‐wide analysis of long non‐coding RNAs in esophageal squamous cell carcinoma reveals their potential role in invasion and metastasis
title_full Genome‐wide analysis of long non‐coding RNAs in esophageal squamous cell carcinoma reveals their potential role in invasion and metastasis
title_fullStr Genome‐wide analysis of long non‐coding RNAs in esophageal squamous cell carcinoma reveals their potential role in invasion and metastasis
title_full_unstemmed Genome‐wide analysis of long non‐coding RNAs in esophageal squamous cell carcinoma reveals their potential role in invasion and metastasis
title_short Genome‐wide analysis of long non‐coding RNAs in esophageal squamous cell carcinoma reveals their potential role in invasion and metastasis
title_sort genome‐wide analysis of long non‐coding rnas in esophageal squamous cell carcinoma reveals their potential role in invasion and metastasis
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6312850/
https://www.ncbi.nlm.nih.gov/pubmed/30390388
http://dx.doi.org/10.1111/1759-7714.12904
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