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Determination of the optimal diabetes duration for bone regeneration experiments in an alloxan-induced diabetic rabbit calvarial defect model

PURPOSE: The purpose of this study was to evaluate the optimal diabetes duration for bone regeneration experiments in an alloxan monohydrate (ALX)–induced diabetic rabbit calvarial defect model by evaluating the association between diabetes duration and bone healing capacity. METHODS: Twenty-four Ne...

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Autores principales: Jeong, Sang-Hun, Jung, Bo Hyun, Yoo, Ki-Yeon, Um, Heung-Sik, Chang, Beom-Seok, Lee, Jae-Kwan, Choi, Won-Youl
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Academy of Periodontology 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6312880/
https://www.ncbi.nlm.nih.gov/pubmed/30619639
http://dx.doi.org/10.5051/jpis.2018.48.6.383
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author Jeong, Sang-Hun
Jung, Bo Hyun
Yoo, Ki-Yeon
Um, Heung-Sik
Chang, Beom-Seok
Lee, Jae-Kwan
Choi, Won-Youl
author_facet Jeong, Sang-Hun
Jung, Bo Hyun
Yoo, Ki-Yeon
Um, Heung-Sik
Chang, Beom-Seok
Lee, Jae-Kwan
Choi, Won-Youl
author_sort Jeong, Sang-Hun
collection PubMed
description PURPOSE: The purpose of this study was to evaluate the optimal diabetes duration for bone regeneration experiments in an alloxan monohydrate (ALX)–induced diabetic rabbit calvarial defect model by evaluating the association between diabetes duration and bone healing capacity. METHODS: Twenty-four New Zealand white rabbits were used. Twenty-two rabbits were injected with 100 mg/kg of ALX to induce experimental diabetes. These rabbits were divided into 4 groups, including a control group and groups with diabetes durations of 1 week (group 1), 2 weeks (group 2), and 4 weeks (group 3). Calvarial defects were created at 1, 2, and 4 weeks after ALX injection and in the control rabbits. Cone-beam computed tomography (CBCT) scanning was performed on the day of surgery and at 2 and 4 weeks after surgery. The rabbits were sacrificed 4 weeks after surgery, followed by histological and immunofluorescence analysis. RESULTS: The diabetic state of all diabetic rabbits was well-maintained throughout the experiment. Reconstructed 3-dimensional CBCT imaging showed more rapid and prominent bone regeneration in the control group than in the experimental groups. Histological staining showed notable bone regeneration in the control group, in contrast to scarce bone formation in the experimental groups. The appearance and immunoreactivity of receptor activator of nuclear factor-kappa B and osteoprotegerin did not show notable differences among the groups. CONCLUSION: ALX administration at 100 mg/kg successfully induced experimental diabetes in rabbits. The effect of diabetes on bone healing was evident when the interval between diabetes induction and the intervention was ≥1 week.
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spelling pubmed-63128802019-01-07 Determination of the optimal diabetes duration for bone regeneration experiments in an alloxan-induced diabetic rabbit calvarial defect model Jeong, Sang-Hun Jung, Bo Hyun Yoo, Ki-Yeon Um, Heung-Sik Chang, Beom-Seok Lee, Jae-Kwan Choi, Won-Youl J Periodontal Implant Sci Research Article PURPOSE: The purpose of this study was to evaluate the optimal diabetes duration for bone regeneration experiments in an alloxan monohydrate (ALX)–induced diabetic rabbit calvarial defect model by evaluating the association between diabetes duration and bone healing capacity. METHODS: Twenty-four New Zealand white rabbits were used. Twenty-two rabbits were injected with 100 mg/kg of ALX to induce experimental diabetes. These rabbits were divided into 4 groups, including a control group and groups with diabetes durations of 1 week (group 1), 2 weeks (group 2), and 4 weeks (group 3). Calvarial defects were created at 1, 2, and 4 weeks after ALX injection and in the control rabbits. Cone-beam computed tomography (CBCT) scanning was performed on the day of surgery and at 2 and 4 weeks after surgery. The rabbits were sacrificed 4 weeks after surgery, followed by histological and immunofluorescence analysis. RESULTS: The diabetic state of all diabetic rabbits was well-maintained throughout the experiment. Reconstructed 3-dimensional CBCT imaging showed more rapid and prominent bone regeneration in the control group than in the experimental groups. Histological staining showed notable bone regeneration in the control group, in contrast to scarce bone formation in the experimental groups. The appearance and immunoreactivity of receptor activator of nuclear factor-kappa B and osteoprotegerin did not show notable differences among the groups. CONCLUSION: ALX administration at 100 mg/kg successfully induced experimental diabetes in rabbits. The effect of diabetes on bone healing was evident when the interval between diabetes induction and the intervention was ≥1 week. Korean Academy of Periodontology 2018-12-27 /pmc/articles/PMC6312880/ /pubmed/30619639 http://dx.doi.org/10.5051/jpis.2018.48.6.383 Text en Copyright © 2018. Korean Academy of Periodontology https://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/).
spellingShingle Research Article
Jeong, Sang-Hun
Jung, Bo Hyun
Yoo, Ki-Yeon
Um, Heung-Sik
Chang, Beom-Seok
Lee, Jae-Kwan
Choi, Won-Youl
Determination of the optimal diabetes duration for bone regeneration experiments in an alloxan-induced diabetic rabbit calvarial defect model
title Determination of the optimal diabetes duration for bone regeneration experiments in an alloxan-induced diabetic rabbit calvarial defect model
title_full Determination of the optimal diabetes duration for bone regeneration experiments in an alloxan-induced diabetic rabbit calvarial defect model
title_fullStr Determination of the optimal diabetes duration for bone regeneration experiments in an alloxan-induced diabetic rabbit calvarial defect model
title_full_unstemmed Determination of the optimal diabetes duration for bone regeneration experiments in an alloxan-induced diabetic rabbit calvarial defect model
title_short Determination of the optimal diabetes duration for bone regeneration experiments in an alloxan-induced diabetic rabbit calvarial defect model
title_sort determination of the optimal diabetes duration for bone regeneration experiments in an alloxan-induced diabetic rabbit calvarial defect model
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6312880/
https://www.ncbi.nlm.nih.gov/pubmed/30619639
http://dx.doi.org/10.5051/jpis.2018.48.6.383
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