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MicroRNA-525 enhances chondrosarcoma malignancy by targeting F-spondin 1
Increasing evidence has suggested that microRNAs (miRNAs; miRs) are extensively involved in the progression of chondrosarcoma (CHS). However, few studies have investigated the functional role of miR-525 in CHS tissues and cells. In the present study, it was discovered that miR-525 levels were decrea...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6313007/ https://www.ncbi.nlm.nih.gov/pubmed/30655830 http://dx.doi.org/10.3892/ol.2018.9711 |
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author | Liu, Bo Song, Xiandong Yan, Zhaowei Yang, Hao Shi, Yingchao Wu, Jintao |
author_facet | Liu, Bo Song, Xiandong Yan, Zhaowei Yang, Hao Shi, Yingchao Wu, Jintao |
author_sort | Liu, Bo |
collection | PubMed |
description | Increasing evidence has suggested that microRNAs (miRNAs; miRs) are extensively involved in the progression of chondrosarcoma (CHS). However, few studies have investigated the functional role of miR-525 in CHS tissues and cells. In the present study, it was discovered that miR-525 levels were decreased in CHS tissues and cells. Dual luciferase assays indicated that F-spondin 1 (SPON1) is a target gene of microRNA (miR)-525. In addition, miR-525 overexpression suppressed SW1353 cell migration and invasion and enhanced SW1353 cell apoptosis. Increased SPON1 expression levels were identified in CHS tissues and cell lines. Furthermore, miR-525 overexpression significantly suppressed the activation of focal adhesion kinase (FAK)/Src/phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K)/protein kinase B (Akt) signaling in CHS cells; this suppression led to SPON1 silencing. In comparison, the SPON1 knockdown-mediated inactivation of FAK/Src/PI3K/Akt signaling was inhibited by inhibiting miR-525. In summary, the present study revealed that decreased miR-525 levels could enhance CHS malignancy as decreased miR-525 binding to the 3′ untranslated region of SPON1 activates FAK/Src/PI3K/Akt signaling. |
format | Online Article Text |
id | pubmed-6313007 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-63130072019-01-17 MicroRNA-525 enhances chondrosarcoma malignancy by targeting F-spondin 1 Liu, Bo Song, Xiandong Yan, Zhaowei Yang, Hao Shi, Yingchao Wu, Jintao Oncol Lett Articles Increasing evidence has suggested that microRNAs (miRNAs; miRs) are extensively involved in the progression of chondrosarcoma (CHS). However, few studies have investigated the functional role of miR-525 in CHS tissues and cells. In the present study, it was discovered that miR-525 levels were decreased in CHS tissues and cells. Dual luciferase assays indicated that F-spondin 1 (SPON1) is a target gene of microRNA (miR)-525. In addition, miR-525 overexpression suppressed SW1353 cell migration and invasion and enhanced SW1353 cell apoptosis. Increased SPON1 expression levels were identified in CHS tissues and cell lines. Furthermore, miR-525 overexpression significantly suppressed the activation of focal adhesion kinase (FAK)/Src/phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K)/protein kinase B (Akt) signaling in CHS cells; this suppression led to SPON1 silencing. In comparison, the SPON1 knockdown-mediated inactivation of FAK/Src/PI3K/Akt signaling was inhibited by inhibiting miR-525. In summary, the present study revealed that decreased miR-525 levels could enhance CHS malignancy as decreased miR-525 binding to the 3′ untranslated region of SPON1 activates FAK/Src/PI3K/Akt signaling. D.A. Spandidos 2019-01 2018-11-15 /pmc/articles/PMC6313007/ /pubmed/30655830 http://dx.doi.org/10.3892/ol.2018.9711 Text en Copyright: © Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Liu, Bo Song, Xiandong Yan, Zhaowei Yang, Hao Shi, Yingchao Wu, Jintao MicroRNA-525 enhances chondrosarcoma malignancy by targeting F-spondin 1 |
title | MicroRNA-525 enhances chondrosarcoma malignancy by targeting F-spondin 1 |
title_full | MicroRNA-525 enhances chondrosarcoma malignancy by targeting F-spondin 1 |
title_fullStr | MicroRNA-525 enhances chondrosarcoma malignancy by targeting F-spondin 1 |
title_full_unstemmed | MicroRNA-525 enhances chondrosarcoma malignancy by targeting F-spondin 1 |
title_short | MicroRNA-525 enhances chondrosarcoma malignancy by targeting F-spondin 1 |
title_sort | microrna-525 enhances chondrosarcoma malignancy by targeting f-spondin 1 |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6313007/ https://www.ncbi.nlm.nih.gov/pubmed/30655830 http://dx.doi.org/10.3892/ol.2018.9711 |
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