Cargando…

Expression pattern and level of ING5 protein in normal and cancer tissues

Inhibitor of growth family 5 (ING5) functions as a type-II tumor suppressor gene and exerts an important role in DNA repair, apoptotic induction, proliferative inhibition, chromatin remodeling and the invasion process. In the present study, immunohistochemistry was performed to characterize the expr...

Descripción completa

Detalles Bibliográficos
Autores principales: Yang, Xue-Feng, Shen, Dao-Fu, Zhao, Shuang, Ren, Tian-Ren, Gao, Yang, Shi, Shuai, Wu, Ji-Cheng, Sun, Hong-Zhi, Zheng, Hua-Chuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6313139/
https://www.ncbi.nlm.nih.gov/pubmed/30655738
http://dx.doi.org/10.3892/ol.2018.9581
_version_ 1783383889142611968
author Yang, Xue-Feng
Shen, Dao-Fu
Zhao, Shuang
Ren, Tian-Ren
Gao, Yang
Shi, Shuai
Wu, Ji-Cheng
Sun, Hong-Zhi
Zheng, Hua-Chuan
author_facet Yang, Xue-Feng
Shen, Dao-Fu
Zhao, Shuang
Ren, Tian-Ren
Gao, Yang
Shi, Shuai
Wu, Ji-Cheng
Sun, Hong-Zhi
Zheng, Hua-Chuan
author_sort Yang, Xue-Feng
collection PubMed
description Inhibitor of growth family 5 (ING5) functions as a type-II tumor suppressor gene and exerts an important role in DNA repair, apoptotic induction, proliferative inhibition, chromatin remodeling and the invasion process. In the present study, immunohistochemistry was performed to characterize the expression profile of ING5 protein on a tissue microarray containing mouse and human normal tissues, and human cancer tissues, including hepatocellular (n=62), renal clear cell (n=62), pancreatic (n=62), esophageal squamous cell (n=45), cervical squamous cell (n=31), breast (n=144), gastric (n=196), colorectal (n=96), endometrial (n=96) and lung carcinoma (n=192). In the mouse tissues, ING5 expression was detected in the cytoplasm of neurons, the nephric tubule and glomerulus, alveolar epithelium, gastrointestinal glands, squamous epithelium of the skin and skeletal muscles. By contrast, ING5 was localized to the cell nucleus in breast tissues. In human tissues, ING5 protein was primarily localized in the cytoplasm. However, ING5 was detected in the cytoplasm and nucleus in various types of normal tissues, including the tongue, stomach, intestine, lung and breast. In total, ING5 expression was detected in 400/986 cancer tissues (40.6%). In the majority of cases, ING5 expression was observed to be restricted to the cytoplasm. However, ING5 was also detected in the nucleus in a number of cancer tissues, including gastric, colorectal and lung carcinoma. Notably, ING5 was more frequently expressed in breast (79.9%), colorectal (56.3%) and endometrial carcinoma (50.0%). The incidence of ING5 expression in hepatocellular carcinoma (14.5%) and pancreatic carcinoma (22.6%) was low. These findings indicate that ING5 may be involved in cell regeneration and be associated with colorectal carcinogenesis.
format Online
Article
Text
id pubmed-6313139
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher D.A. Spandidos
record_format MEDLINE/PubMed
spelling pubmed-63131392019-01-17 Expression pattern and level of ING5 protein in normal and cancer tissues Yang, Xue-Feng Shen, Dao-Fu Zhao, Shuang Ren, Tian-Ren Gao, Yang Shi, Shuai Wu, Ji-Cheng Sun, Hong-Zhi Zheng, Hua-Chuan Oncol Lett Articles Inhibitor of growth family 5 (ING5) functions as a type-II tumor suppressor gene and exerts an important role in DNA repair, apoptotic induction, proliferative inhibition, chromatin remodeling and the invasion process. In the present study, immunohistochemistry was performed to characterize the expression profile of ING5 protein on a tissue microarray containing mouse and human normal tissues, and human cancer tissues, including hepatocellular (n=62), renal clear cell (n=62), pancreatic (n=62), esophageal squamous cell (n=45), cervical squamous cell (n=31), breast (n=144), gastric (n=196), colorectal (n=96), endometrial (n=96) and lung carcinoma (n=192). In the mouse tissues, ING5 expression was detected in the cytoplasm of neurons, the nephric tubule and glomerulus, alveolar epithelium, gastrointestinal glands, squamous epithelium of the skin and skeletal muscles. By contrast, ING5 was localized to the cell nucleus in breast tissues. In human tissues, ING5 protein was primarily localized in the cytoplasm. However, ING5 was detected in the cytoplasm and nucleus in various types of normal tissues, including the tongue, stomach, intestine, lung and breast. In total, ING5 expression was detected in 400/986 cancer tissues (40.6%). In the majority of cases, ING5 expression was observed to be restricted to the cytoplasm. However, ING5 was also detected in the nucleus in a number of cancer tissues, including gastric, colorectal and lung carcinoma. Notably, ING5 was more frequently expressed in breast (79.9%), colorectal (56.3%) and endometrial carcinoma (50.0%). The incidence of ING5 expression in hepatocellular carcinoma (14.5%) and pancreatic carcinoma (22.6%) was low. These findings indicate that ING5 may be involved in cell regeneration and be associated with colorectal carcinogenesis. D.A. Spandidos 2019-01 2018-10-16 /pmc/articles/PMC6313139/ /pubmed/30655738 http://dx.doi.org/10.3892/ol.2018.9581 Text en Copyright: © Yang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Yang, Xue-Feng
Shen, Dao-Fu
Zhao, Shuang
Ren, Tian-Ren
Gao, Yang
Shi, Shuai
Wu, Ji-Cheng
Sun, Hong-Zhi
Zheng, Hua-Chuan
Expression pattern and level of ING5 protein in normal and cancer tissues
title Expression pattern and level of ING5 protein in normal and cancer tissues
title_full Expression pattern and level of ING5 protein in normal and cancer tissues
title_fullStr Expression pattern and level of ING5 protein in normal and cancer tissues
title_full_unstemmed Expression pattern and level of ING5 protein in normal and cancer tissues
title_short Expression pattern and level of ING5 protein in normal and cancer tissues
title_sort expression pattern and level of ing5 protein in normal and cancer tissues
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6313139/
https://www.ncbi.nlm.nih.gov/pubmed/30655738
http://dx.doi.org/10.3892/ol.2018.9581
work_keys_str_mv AT yangxuefeng expressionpatternandlevelofing5proteininnormalandcancertissues
AT shendaofu expressionpatternandlevelofing5proteininnormalandcancertissues
AT zhaoshuang expressionpatternandlevelofing5proteininnormalandcancertissues
AT rentianren expressionpatternandlevelofing5proteininnormalandcancertissues
AT gaoyang expressionpatternandlevelofing5proteininnormalandcancertissues
AT shishuai expressionpatternandlevelofing5proteininnormalandcancertissues
AT wujicheng expressionpatternandlevelofing5proteininnormalandcancertissues
AT sunhongzhi expressionpatternandlevelofing5proteininnormalandcancertissues
AT zhenghuachuan expressionpatternandlevelofing5proteininnormalandcancertissues