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Antimicrobial Resistance in Class 1 Integron-Positive Shiga Toxin-Producing Escherichia coli Isolated from Cattle, Pigs, Food and Farm Environment

The aim of this study was to investigate the presence of class 1 integrons in a collection of Shiga toxin-producing Escherichia coli (STEC) from different origins and to characterize pheno- and genotypically the antimicrobial resistance associated to them. A collection of 649 isolates were screened...

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Autores principales: Colello, Rocío, Krüger, Alejandra, Conza, José Di, Rossen, John W. A., Friedrich, Alexander W., Gutkind, Gabriel, Etcheverría, Analía I., Padola, Nora L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6313391/
https://www.ncbi.nlm.nih.gov/pubmed/30274159
http://dx.doi.org/10.3390/microorganisms6040099
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author Colello, Rocío
Krüger, Alejandra
Conza, José Di
Rossen, John W. A.
Friedrich, Alexander W.
Gutkind, Gabriel
Etcheverría, Analía I.
Padola, Nora L.
author_facet Colello, Rocío
Krüger, Alejandra
Conza, José Di
Rossen, John W. A.
Friedrich, Alexander W.
Gutkind, Gabriel
Etcheverría, Analía I.
Padola, Nora L.
author_sort Colello, Rocío
collection PubMed
description The aim of this study was to investigate the presence of class 1 integrons in a collection of Shiga toxin-producing Escherichia coli (STEC) from different origins and to characterize pheno- and genotypically the antimicrobial resistance associated to them. A collection of 649 isolates were screened for the class 1 integrase gene (intI1) by Polymerase chain reaction The variable region of class 1 integrons was amplified and sequenced. Positive strains were evaluated for the presence of antimicrobial resistance genes with microarray and for antimicrobial susceptibility by the disk diffusion method. Seven out of 649 STEC strains some to serogroups, O26, O103 and O130 isolated from cattle, chicken burger, farm environment and pigs were identified as positive for intl1. Different arrangements of gene cassettes were detected in the variable region of class 1 integron: dfrA16, aadA23 and dfrA1-aadA1. In almost all strains, phenotypic resistance to streptomycin, tetracycline, trimethoprim/sulfamethoxazole, and sulfisoxazole was observed. Microarray analyses showed that most of the isolates carried four or more antimicrobial resistance markers and STEC strains were categorized as Multridrug-resistant. Although antimicrobials are not usually used in the treatment of STEC infections, the presence of Multridrug-resistant in isolates collected from farm and food represents a risk for animal and human health.
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spelling pubmed-63133912019-01-04 Antimicrobial Resistance in Class 1 Integron-Positive Shiga Toxin-Producing Escherichia coli Isolated from Cattle, Pigs, Food and Farm Environment Colello, Rocío Krüger, Alejandra Conza, José Di Rossen, John W. A. Friedrich, Alexander W. Gutkind, Gabriel Etcheverría, Analía I. Padola, Nora L. Microorganisms Brief Report The aim of this study was to investigate the presence of class 1 integrons in a collection of Shiga toxin-producing Escherichia coli (STEC) from different origins and to characterize pheno- and genotypically the antimicrobial resistance associated to them. A collection of 649 isolates were screened for the class 1 integrase gene (intI1) by Polymerase chain reaction The variable region of class 1 integrons was amplified and sequenced. Positive strains were evaluated for the presence of antimicrobial resistance genes with microarray and for antimicrobial susceptibility by the disk diffusion method. Seven out of 649 STEC strains some to serogroups, O26, O103 and O130 isolated from cattle, chicken burger, farm environment and pigs were identified as positive for intl1. Different arrangements of gene cassettes were detected in the variable region of class 1 integron: dfrA16, aadA23 and dfrA1-aadA1. In almost all strains, phenotypic resistance to streptomycin, tetracycline, trimethoprim/sulfamethoxazole, and sulfisoxazole was observed. Microarray analyses showed that most of the isolates carried four or more antimicrobial resistance markers and STEC strains were categorized as Multridrug-resistant. Although antimicrobials are not usually used in the treatment of STEC infections, the presence of Multridrug-resistant in isolates collected from farm and food represents a risk for animal and human health. MDPI 2018-09-28 /pmc/articles/PMC6313391/ /pubmed/30274159 http://dx.doi.org/10.3390/microorganisms6040099 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Colello, Rocío
Krüger, Alejandra
Conza, José Di
Rossen, John W. A.
Friedrich, Alexander W.
Gutkind, Gabriel
Etcheverría, Analía I.
Padola, Nora L.
Antimicrobial Resistance in Class 1 Integron-Positive Shiga Toxin-Producing Escherichia coli Isolated from Cattle, Pigs, Food and Farm Environment
title Antimicrobial Resistance in Class 1 Integron-Positive Shiga Toxin-Producing Escherichia coli Isolated from Cattle, Pigs, Food and Farm Environment
title_full Antimicrobial Resistance in Class 1 Integron-Positive Shiga Toxin-Producing Escherichia coli Isolated from Cattle, Pigs, Food and Farm Environment
title_fullStr Antimicrobial Resistance in Class 1 Integron-Positive Shiga Toxin-Producing Escherichia coli Isolated from Cattle, Pigs, Food and Farm Environment
title_full_unstemmed Antimicrobial Resistance in Class 1 Integron-Positive Shiga Toxin-Producing Escherichia coli Isolated from Cattle, Pigs, Food and Farm Environment
title_short Antimicrobial Resistance in Class 1 Integron-Positive Shiga Toxin-Producing Escherichia coli Isolated from Cattle, Pigs, Food and Farm Environment
title_sort antimicrobial resistance in class 1 integron-positive shiga toxin-producing escherichia coli isolated from cattle, pigs, food and farm environment
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6313391/
https://www.ncbi.nlm.nih.gov/pubmed/30274159
http://dx.doi.org/10.3390/microorganisms6040099
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