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Characterization and Antimicrobial Susceptibility of Lactococcus lactis Isolated from Endodontic Infections in Ouagadougou, Burkina Faso
Background: This study aimed to characterize and test the antimicrobial susceptibility of Lactococcus lactis isolated in endodontic infections in Burkina Faso. Material and methods: This was a prospective study conducted at the Municipal Oral Health Center of Ouagadougou, Burkina Faso, from June to...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6313549/ https://www.ncbi.nlm.nih.gov/pubmed/30544668 http://dx.doi.org/10.3390/dj6040069 |
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author | Kaboré, Wendpoulomdé Aimé Désiré Dembélé, René Bagré, Touwendsida Serge Konaté, Ali Boisramé, Sylvie Chevalier, Valérie Konsem, Tarcissus Traoré, Alfred S. Barro, Nicolas |
author_facet | Kaboré, Wendpoulomdé Aimé Désiré Dembélé, René Bagré, Touwendsida Serge Konaté, Ali Boisramé, Sylvie Chevalier, Valérie Konsem, Tarcissus Traoré, Alfred S. Barro, Nicolas |
author_sort | Kaboré, Wendpoulomdé Aimé Désiré |
collection | PubMed |
description | Background: This study aimed to characterize and test the antimicrobial susceptibility of Lactococcus lactis isolated in endodontic infections in Burkina Faso. Material and methods: This was a prospective study conducted at the Municipal Oral Health Center of Ouagadougou, Burkina Faso, from June to October 2014. Clinical data were collected using a questionnaire form. The method of streaking on selective medium was used to isolate bacteria. Identification was made using the API 20 Strep gallery. Antibiotic susceptibility was performed by the diffusion method on solid medium. Results: One hundred and twenty-five (125) patients were received with a significant proportion from the age group of 19 to 40 years (55.2%). Apical periodontitis accounted for 50.4% and cellulitis for 49.6% of cases. Lactococcus lactis ssp. lactis was identified in five exudate samples. Isolates were 100% resistant to cefixime and metronidazole, 80% to ceftriaxone, cefuroxime, cefotaxime, chloramphenicol and 60% to penicillin G, amoxicillin, amoxicillin clavulanic acid. A multidrug resistance of more than three families of antibiotics was noticed. No strains produced extended spectrum ß-lactamases. Conclusion: Lactococcus lactis is part of endodontic biofilm. The reported strong antibiotic resistance involving endodontic therapy will focus on the effect of the disinfectant solution and the mechanical action of the canal instruments. |
format | Online Article Text |
id | pubmed-6313549 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-63135492019-01-04 Characterization and Antimicrobial Susceptibility of Lactococcus lactis Isolated from Endodontic Infections in Ouagadougou, Burkina Faso Kaboré, Wendpoulomdé Aimé Désiré Dembélé, René Bagré, Touwendsida Serge Konaté, Ali Boisramé, Sylvie Chevalier, Valérie Konsem, Tarcissus Traoré, Alfred S. Barro, Nicolas Dent J (Basel) Article Background: This study aimed to characterize and test the antimicrobial susceptibility of Lactococcus lactis isolated in endodontic infections in Burkina Faso. Material and methods: This was a prospective study conducted at the Municipal Oral Health Center of Ouagadougou, Burkina Faso, from June to October 2014. Clinical data were collected using a questionnaire form. The method of streaking on selective medium was used to isolate bacteria. Identification was made using the API 20 Strep gallery. Antibiotic susceptibility was performed by the diffusion method on solid medium. Results: One hundred and twenty-five (125) patients were received with a significant proportion from the age group of 19 to 40 years (55.2%). Apical periodontitis accounted for 50.4% and cellulitis for 49.6% of cases. Lactococcus lactis ssp. lactis was identified in five exudate samples. Isolates were 100% resistant to cefixime and metronidazole, 80% to ceftriaxone, cefuroxime, cefotaxime, chloramphenicol and 60% to penicillin G, amoxicillin, amoxicillin clavulanic acid. A multidrug resistance of more than three families of antibiotics was noticed. No strains produced extended spectrum ß-lactamases. Conclusion: Lactococcus lactis is part of endodontic biofilm. The reported strong antibiotic resistance involving endodontic therapy will focus on the effect of the disinfectant solution and the mechanical action of the canal instruments. MDPI 2018-12-10 /pmc/articles/PMC6313549/ /pubmed/30544668 http://dx.doi.org/10.3390/dj6040069 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kaboré, Wendpoulomdé Aimé Désiré Dembélé, René Bagré, Touwendsida Serge Konaté, Ali Boisramé, Sylvie Chevalier, Valérie Konsem, Tarcissus Traoré, Alfred S. Barro, Nicolas Characterization and Antimicrobial Susceptibility of Lactococcus lactis Isolated from Endodontic Infections in Ouagadougou, Burkina Faso |
title | Characterization and Antimicrobial Susceptibility of Lactococcus lactis Isolated from Endodontic Infections in Ouagadougou, Burkina Faso |
title_full | Characterization and Antimicrobial Susceptibility of Lactococcus lactis Isolated from Endodontic Infections in Ouagadougou, Burkina Faso |
title_fullStr | Characterization and Antimicrobial Susceptibility of Lactococcus lactis Isolated from Endodontic Infections in Ouagadougou, Burkina Faso |
title_full_unstemmed | Characterization and Antimicrobial Susceptibility of Lactococcus lactis Isolated from Endodontic Infections in Ouagadougou, Burkina Faso |
title_short | Characterization and Antimicrobial Susceptibility of Lactococcus lactis Isolated from Endodontic Infections in Ouagadougou, Burkina Faso |
title_sort | characterization and antimicrobial susceptibility of lactococcus lactis isolated from endodontic infections in ouagadougou, burkina faso |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6313549/ https://www.ncbi.nlm.nih.gov/pubmed/30544668 http://dx.doi.org/10.3390/dj6040069 |
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