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Susceptibility of Multidrug-Resistant Bacteria, Isolated from Water and Plants in Nigeria, to Ceragenins
The continuous emergence of multidrug resistant pathogens is a major global health concern. Although antimicrobial peptides (AMPs) have shown promise as a possible means of combatting multidrug resistant strains without readily engendering resistance, costs of production and targeting by proteases l...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6313750/ https://www.ncbi.nlm.nih.gov/pubmed/30563216 http://dx.doi.org/10.3390/ijerph15122758 |
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author | Hashemi, Marjan M. Mmuoegbulam, Augusta O. Holden, Brett S. Coburn, Jordan Wilson, John Taylor, Maddison F. Reiley, Joseph Baradaran, Darius Stenquist, Tania Deng, Shenglou Savage, Paul B. |
author_facet | Hashemi, Marjan M. Mmuoegbulam, Augusta O. Holden, Brett S. Coburn, Jordan Wilson, John Taylor, Maddison F. Reiley, Joseph Baradaran, Darius Stenquist, Tania Deng, Shenglou Savage, Paul B. |
author_sort | Hashemi, Marjan M. |
collection | PubMed |
description | The continuous emergence of multidrug resistant pathogens is a major global health concern. Although antimicrobial peptides (AMPs) have shown promise as a possible means of combatting multidrug resistant strains without readily engendering resistance, costs of production and targeting by proteases limit their utility. Ceragenins are non-peptide AMP mimics that overcome these shortcomings while retaining broad-spectrum antimicrobial activity. To further characterize the antibacterial activities of ceragenins, their activities against a collection of environmental isolates of bacteria were determined. These isolates were isolated in Nigeria from plants and water. Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) of selected ceragenins and currently available antimicrobials against these isolates were measured to determine resistance patterns. Using scanning electron microscopy (SEM), we examined the morphological changes in bacterial membranes following treatment with ceragenins. Finally, we investigated the effectiveness of ceragenins in inhibiting biofilm formation and destroying established biofilms. We found that, despite high resistance to many currently available antimicrobials, including colistin, environmental isolates in planktonic and biofilm forms remain susceptible to ceragenins. Additionally, SEM and confocal images of ceragenin-treated cells confirmed the effective antibacterial and antibiofilm activity of ceragenins. |
format | Online Article Text |
id | pubmed-6313750 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-63137502019-06-17 Susceptibility of Multidrug-Resistant Bacteria, Isolated from Water and Plants in Nigeria, to Ceragenins Hashemi, Marjan M. Mmuoegbulam, Augusta O. Holden, Brett S. Coburn, Jordan Wilson, John Taylor, Maddison F. Reiley, Joseph Baradaran, Darius Stenquist, Tania Deng, Shenglou Savage, Paul B. Int J Environ Res Public Health Article The continuous emergence of multidrug resistant pathogens is a major global health concern. Although antimicrobial peptides (AMPs) have shown promise as a possible means of combatting multidrug resistant strains without readily engendering resistance, costs of production and targeting by proteases limit their utility. Ceragenins are non-peptide AMP mimics that overcome these shortcomings while retaining broad-spectrum antimicrobial activity. To further characterize the antibacterial activities of ceragenins, their activities against a collection of environmental isolates of bacteria were determined. These isolates were isolated in Nigeria from plants and water. Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) of selected ceragenins and currently available antimicrobials against these isolates were measured to determine resistance patterns. Using scanning electron microscopy (SEM), we examined the morphological changes in bacterial membranes following treatment with ceragenins. Finally, we investigated the effectiveness of ceragenins in inhibiting biofilm formation and destroying established biofilms. We found that, despite high resistance to many currently available antimicrobials, including colistin, environmental isolates in planktonic and biofilm forms remain susceptible to ceragenins. Additionally, SEM and confocal images of ceragenin-treated cells confirmed the effective antibacterial and antibiofilm activity of ceragenins. MDPI 2018-12-06 2018-12 /pmc/articles/PMC6313750/ /pubmed/30563216 http://dx.doi.org/10.3390/ijerph15122758 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hashemi, Marjan M. Mmuoegbulam, Augusta O. Holden, Brett S. Coburn, Jordan Wilson, John Taylor, Maddison F. Reiley, Joseph Baradaran, Darius Stenquist, Tania Deng, Shenglou Savage, Paul B. Susceptibility of Multidrug-Resistant Bacteria, Isolated from Water and Plants in Nigeria, to Ceragenins |
title | Susceptibility of Multidrug-Resistant Bacteria, Isolated from Water and Plants in Nigeria, to Ceragenins |
title_full | Susceptibility of Multidrug-Resistant Bacteria, Isolated from Water and Plants in Nigeria, to Ceragenins |
title_fullStr | Susceptibility of Multidrug-Resistant Bacteria, Isolated from Water and Plants in Nigeria, to Ceragenins |
title_full_unstemmed | Susceptibility of Multidrug-Resistant Bacteria, Isolated from Water and Plants in Nigeria, to Ceragenins |
title_short | Susceptibility of Multidrug-Resistant Bacteria, Isolated from Water and Plants in Nigeria, to Ceragenins |
title_sort | susceptibility of multidrug-resistant bacteria, isolated from water and plants in nigeria, to ceragenins |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6313750/ https://www.ncbi.nlm.nih.gov/pubmed/30563216 http://dx.doi.org/10.3390/ijerph15122758 |
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