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Developmental Stage and Shape of Embryo Determine the Efficacy of Embryo Rescue in Introgressing Orange/Yellow Color and Anthocyanin Genes of Brassica Species

Vegetables in Brassica are some of the world’s most commonly cultivated plants and have a wide range of consumable plant organs. Improvement of this group of vegetables is limited at the species level due to limited genetic variability. Interspecies hybridization could be a powerful alternate tool f...

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Autores principales: Pen, Sreyvatey, Nath, Ujjal Kumar, Song, Samnang, Goswami, Gayatri, Lee, Ji-Hee, Jung, Hee-Jeong, Kim, Hoy-Taek, Park, Jong-In, Nou, Ill-Sup
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6313868/
https://www.ncbi.nlm.nih.gov/pubmed/30428562
http://dx.doi.org/10.3390/plants7040099
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author Pen, Sreyvatey
Nath, Ujjal Kumar
Song, Samnang
Goswami, Gayatri
Lee, Ji-Hee
Jung, Hee-Jeong
Kim, Hoy-Taek
Park, Jong-In
Nou, Ill-Sup
author_facet Pen, Sreyvatey
Nath, Ujjal Kumar
Song, Samnang
Goswami, Gayatri
Lee, Ji-Hee
Jung, Hee-Jeong
Kim, Hoy-Taek
Park, Jong-In
Nou, Ill-Sup
author_sort Pen, Sreyvatey
collection PubMed
description Vegetables in Brassica are some of the world’s most commonly cultivated plants and have a wide range of consumable plant organs. Improvement of this group of vegetables is limited at the species level due to limited genetic variability. Interspecies hybridization could be a powerful alternate tool for broadening the genetic variability of target traits. Embryo rescue technique is necessarily practiced in interspecies hybridization for protecting embryos from premature abortion. However, its success depends on the age of ovaries, shape of embryos, and the effect of female genotype. In this study, we carried out a wide range of interspecies crossing for introgressing target traits (orange/yellow color in cabbage and anthocyanin in Chinese cabbage) and optimizing the appropriate age of ovaries, the shape of embryo, and the suitable genotypes of such crosses. We observed that 15 DAP (days after pollination) was the best for embryo rescue in the diploid-diploid (Brassica rapa × B. oleracea) crosses, while 20 DAP was optimum for amphidiploid-diploid (B. napus/B. juncea × B. rapa) crosses. Cotyledonary shape of embryos and genotypes of amphidiploid species were the best for successful plant regeneration in interspecies crosses. We successfully selected plants with desired orange/yellow inner leaves for cabbage and higher anthocyanin in Chinese cabbage. The results of this study have the potential to be applied for the efficient production of interspecific hybrids and to develop Brassica vegetables with new traits, which could have potential for the enrichment of the human diet.
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spelling pubmed-63138682019-01-07 Developmental Stage and Shape of Embryo Determine the Efficacy of Embryo Rescue in Introgressing Orange/Yellow Color and Anthocyanin Genes of Brassica Species Pen, Sreyvatey Nath, Ujjal Kumar Song, Samnang Goswami, Gayatri Lee, Ji-Hee Jung, Hee-Jeong Kim, Hoy-Taek Park, Jong-In Nou, Ill-Sup Plants (Basel) Article Vegetables in Brassica are some of the world’s most commonly cultivated plants and have a wide range of consumable plant organs. Improvement of this group of vegetables is limited at the species level due to limited genetic variability. Interspecies hybridization could be a powerful alternate tool for broadening the genetic variability of target traits. Embryo rescue technique is necessarily practiced in interspecies hybridization for protecting embryos from premature abortion. However, its success depends on the age of ovaries, shape of embryos, and the effect of female genotype. In this study, we carried out a wide range of interspecies crossing for introgressing target traits (orange/yellow color in cabbage and anthocyanin in Chinese cabbage) and optimizing the appropriate age of ovaries, the shape of embryo, and the suitable genotypes of such crosses. We observed that 15 DAP (days after pollination) was the best for embryo rescue in the diploid-diploid (Brassica rapa × B. oleracea) crosses, while 20 DAP was optimum for amphidiploid-diploid (B. napus/B. juncea × B. rapa) crosses. Cotyledonary shape of embryos and genotypes of amphidiploid species were the best for successful plant regeneration in interspecies crosses. We successfully selected plants with desired orange/yellow inner leaves for cabbage and higher anthocyanin in Chinese cabbage. The results of this study have the potential to be applied for the efficient production of interspecific hybrids and to develop Brassica vegetables with new traits, which could have potential for the enrichment of the human diet. MDPI 2018-11-13 /pmc/articles/PMC6313868/ /pubmed/30428562 http://dx.doi.org/10.3390/plants7040099 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pen, Sreyvatey
Nath, Ujjal Kumar
Song, Samnang
Goswami, Gayatri
Lee, Ji-Hee
Jung, Hee-Jeong
Kim, Hoy-Taek
Park, Jong-In
Nou, Ill-Sup
Developmental Stage and Shape of Embryo Determine the Efficacy of Embryo Rescue in Introgressing Orange/Yellow Color and Anthocyanin Genes of Brassica Species
title Developmental Stage and Shape of Embryo Determine the Efficacy of Embryo Rescue in Introgressing Orange/Yellow Color and Anthocyanin Genes of Brassica Species
title_full Developmental Stage and Shape of Embryo Determine the Efficacy of Embryo Rescue in Introgressing Orange/Yellow Color and Anthocyanin Genes of Brassica Species
title_fullStr Developmental Stage and Shape of Embryo Determine the Efficacy of Embryo Rescue in Introgressing Orange/Yellow Color and Anthocyanin Genes of Brassica Species
title_full_unstemmed Developmental Stage and Shape of Embryo Determine the Efficacy of Embryo Rescue in Introgressing Orange/Yellow Color and Anthocyanin Genes of Brassica Species
title_short Developmental Stage and Shape of Embryo Determine the Efficacy of Embryo Rescue in Introgressing Orange/Yellow Color and Anthocyanin Genes of Brassica Species
title_sort developmental stage and shape of embryo determine the efficacy of embryo rescue in introgressing orange/yellow color and anthocyanin genes of brassica species
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6313868/
https://www.ncbi.nlm.nih.gov/pubmed/30428562
http://dx.doi.org/10.3390/plants7040099
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