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Cannabidiol Affects Extracellular Vesicle Release, miR21 and miR126, and Reduces Prohibitin Protein in Glioblastoma Multiforme Cells()()

Glioblastoma multiforme (GBM) is the most common and aggressive form of primary malignant brain tumor in adults, with poor prognosis. Extracellular vesicles (EVs) are key-mediators for cellular communication through transfer of proteins and genetic material. Cancers, such as GBM, use EV release for...

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Autores principales: Kosgodage, Uchini S., Uysal-Onganer, Pinar, MacLatchy, Amy, Mould, Rhys, Nunn, Alistair V., Guy, Geoffrey W., Kraev, Igor, Chatterton, Nicholas P., Thomas, E. Louise, Inal, Jameel M., Bell, Jimmy D., Lange, Sigrun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Neoplasia Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6314156/
https://www.ncbi.nlm.nih.gov/pubmed/30597288
http://dx.doi.org/10.1016/j.tranon.2018.12.004
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author Kosgodage, Uchini S.
Uysal-Onganer, Pinar
MacLatchy, Amy
Mould, Rhys
Nunn, Alistair V.
Guy, Geoffrey W.
Kraev, Igor
Chatterton, Nicholas P.
Thomas, E. Louise
Inal, Jameel M.
Bell, Jimmy D.
Lange, Sigrun
author_facet Kosgodage, Uchini S.
Uysal-Onganer, Pinar
MacLatchy, Amy
Mould, Rhys
Nunn, Alistair V.
Guy, Geoffrey W.
Kraev, Igor
Chatterton, Nicholas P.
Thomas, E. Louise
Inal, Jameel M.
Bell, Jimmy D.
Lange, Sigrun
author_sort Kosgodage, Uchini S.
collection PubMed
description Glioblastoma multiforme (GBM) is the most common and aggressive form of primary malignant brain tumor in adults, with poor prognosis. Extracellular vesicles (EVs) are key-mediators for cellular communication through transfer of proteins and genetic material. Cancers, such as GBM, use EV release for drug-efflux, pro-oncogenic signaling, invasion and immunosuppression; thus the modulation of EV release and cargo is of considerable clinical relevance. As EV-inhibitors have been shown to increase sensitivity of cancer cells to chemotherapy, and we recently showed that cannabidiol (CBD) is such an EV-modulator, we investigated whether CBD affects EV profile in GBM cells in the presence and absence of temozolomide (TMZ). Compared to controls, CBD-treated cells released EVs containing lower levels of pro-oncogenic miR21 and increased levels of anti-oncogenic miR126; these effects were greater than with TMZ alone. In addition, prohibitin (PHB), a multifunctional protein with mitochondrial protective properties and chemoresistant functions, was reduced in GBM cells following 1 h CBD treatment. This data suggests that CBD may, via modulation of EVs and PHB, act as an adjunct to enhance treatment efficacy in GBM, supporting evidence for efficacy of cannabinoids in GBM.
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spelling pubmed-63141562019-01-08 Cannabidiol Affects Extracellular Vesicle Release, miR21 and miR126, and Reduces Prohibitin Protein in Glioblastoma Multiforme Cells()() Kosgodage, Uchini S. Uysal-Onganer, Pinar MacLatchy, Amy Mould, Rhys Nunn, Alistair V. Guy, Geoffrey W. Kraev, Igor Chatterton, Nicholas P. Thomas, E. Louise Inal, Jameel M. Bell, Jimmy D. Lange, Sigrun Transl Oncol Original article Glioblastoma multiforme (GBM) is the most common and aggressive form of primary malignant brain tumor in adults, with poor prognosis. Extracellular vesicles (EVs) are key-mediators for cellular communication through transfer of proteins and genetic material. Cancers, such as GBM, use EV release for drug-efflux, pro-oncogenic signaling, invasion and immunosuppression; thus the modulation of EV release and cargo is of considerable clinical relevance. As EV-inhibitors have been shown to increase sensitivity of cancer cells to chemotherapy, and we recently showed that cannabidiol (CBD) is such an EV-modulator, we investigated whether CBD affects EV profile in GBM cells in the presence and absence of temozolomide (TMZ). Compared to controls, CBD-treated cells released EVs containing lower levels of pro-oncogenic miR21 and increased levels of anti-oncogenic miR126; these effects were greater than with TMZ alone. In addition, prohibitin (PHB), a multifunctional protein with mitochondrial protective properties and chemoresistant functions, was reduced in GBM cells following 1 h CBD treatment. This data suggests that CBD may, via modulation of EVs and PHB, act as an adjunct to enhance treatment efficacy in GBM, supporting evidence for efficacy of cannabinoids in GBM. Neoplasia Press 2018-12-28 /pmc/articles/PMC6314156/ /pubmed/30597288 http://dx.doi.org/10.1016/j.tranon.2018.12.004 Text en © 2018 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original article
Kosgodage, Uchini S.
Uysal-Onganer, Pinar
MacLatchy, Amy
Mould, Rhys
Nunn, Alistair V.
Guy, Geoffrey W.
Kraev, Igor
Chatterton, Nicholas P.
Thomas, E. Louise
Inal, Jameel M.
Bell, Jimmy D.
Lange, Sigrun
Cannabidiol Affects Extracellular Vesicle Release, miR21 and miR126, and Reduces Prohibitin Protein in Glioblastoma Multiforme Cells()()
title Cannabidiol Affects Extracellular Vesicle Release, miR21 and miR126, and Reduces Prohibitin Protein in Glioblastoma Multiforme Cells()()
title_full Cannabidiol Affects Extracellular Vesicle Release, miR21 and miR126, and Reduces Prohibitin Protein in Glioblastoma Multiforme Cells()()
title_fullStr Cannabidiol Affects Extracellular Vesicle Release, miR21 and miR126, and Reduces Prohibitin Protein in Glioblastoma Multiforme Cells()()
title_full_unstemmed Cannabidiol Affects Extracellular Vesicle Release, miR21 and miR126, and Reduces Prohibitin Protein in Glioblastoma Multiforme Cells()()
title_short Cannabidiol Affects Extracellular Vesicle Release, miR21 and miR126, and Reduces Prohibitin Protein in Glioblastoma Multiforme Cells()()
title_sort cannabidiol affects extracellular vesicle release, mir21 and mir126, and reduces prohibitin protein in glioblastoma multiforme cells()()
topic Original article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6314156/
https://www.ncbi.nlm.nih.gov/pubmed/30597288
http://dx.doi.org/10.1016/j.tranon.2018.12.004
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