Imaging cellular ultrastructures using expansion microscopy (U-ExM)

The attribution of a protein to an ultrastructural element by optical microscopy represents a major challenge in biology. Here, we report a method of near-native expansion microscopy (U-ExM), enabling the visualization of preserved ultrastructures of macromolecules by optical microscopy. Combined wi...

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Detalles Bibliográficos
Autores principales: Gambarotto, D., Zwettler, F. U., Le Guennec, M., Schmidt-Cernohorska, M., Fortun, D., Borgers, S., Heine, J., Schloetel, J. G., Reuss, M., Unser, M., Boyden, E. S., Sauer, M., Hamel, V., Guichard, P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6314451/
https://www.ncbi.nlm.nih.gov/pubmed/30559430
http://dx.doi.org/10.1038/s41592-018-0238-1
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author Gambarotto, D.
Zwettler, F. U.
Le Guennec, M.
Schmidt-Cernohorska, M.
Fortun, D.
Borgers, S.
Heine, J.
Schloetel, J. G.
Reuss, M.
Unser, M.
Boyden, E. S.
Sauer, M.
Hamel, V.
Guichard, P.
author_facet Gambarotto, D.
Zwettler, F. U.
Le Guennec, M.
Schmidt-Cernohorska, M.
Fortun, D.
Borgers, S.
Heine, J.
Schloetel, J. G.
Reuss, M.
Unser, M.
Boyden, E. S.
Sauer, M.
Hamel, V.
Guichard, P.
author_sort Gambarotto, D.
collection PubMed
description The attribution of a protein to an ultrastructural element by optical microscopy represents a major challenge in biology. Here, we report a method of near-native expansion microscopy (U-ExM), enabling the visualization of preserved ultrastructures of macromolecules by optical microscopy. Combined with super-resolution, U-ExM unveiled the centriolar chirality, only visualizable by electron microscopy. We demonstrate the general applicability of U-ExM by imaging different cellular structures including microtubules and mitochondria in cellulo.
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spelling pubmed-63144512019-06-17 Imaging cellular ultrastructures using expansion microscopy (U-ExM) Gambarotto, D. Zwettler, F. U. Le Guennec, M. Schmidt-Cernohorska, M. Fortun, D. Borgers, S. Heine, J. Schloetel, J. G. Reuss, M. Unser, M. Boyden, E. S. Sauer, M. Hamel, V. Guichard, P. Nat Methods Article The attribution of a protein to an ultrastructural element by optical microscopy represents a major challenge in biology. Here, we report a method of near-native expansion microscopy (U-ExM), enabling the visualization of preserved ultrastructures of macromolecules by optical microscopy. Combined with super-resolution, U-ExM unveiled the centriolar chirality, only visualizable by electron microscopy. We demonstrate the general applicability of U-ExM by imaging different cellular structures including microtubules and mitochondria in cellulo. 2018-12-17 2019-01 /pmc/articles/PMC6314451/ /pubmed/30559430 http://dx.doi.org/10.1038/s41592-018-0238-1 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Gambarotto, D.
Zwettler, F. U.
Le Guennec, M.
Schmidt-Cernohorska, M.
Fortun, D.
Borgers, S.
Heine, J.
Schloetel, J. G.
Reuss, M.
Unser, M.
Boyden, E. S.
Sauer, M.
Hamel, V.
Guichard, P.
Imaging cellular ultrastructures using expansion microscopy (U-ExM)
title Imaging cellular ultrastructures using expansion microscopy (U-ExM)
title_full Imaging cellular ultrastructures using expansion microscopy (U-ExM)
title_fullStr Imaging cellular ultrastructures using expansion microscopy (U-ExM)
title_full_unstemmed Imaging cellular ultrastructures using expansion microscopy (U-ExM)
title_short Imaging cellular ultrastructures using expansion microscopy (U-ExM)
title_sort imaging cellular ultrastructures using expansion microscopy (u-exm)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6314451/
https://www.ncbi.nlm.nih.gov/pubmed/30559430
http://dx.doi.org/10.1038/s41592-018-0238-1
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