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Impact of glucose-6-phosphate dehydrogenase deficiency on dengue infection in Myanmar children

Glucose-6-phosphate dehydrogenase (G6PD) deficiency may affect the clinical presentation of dengue due to the altered redox state in immune cells. We aimed to determine the association between G6PD deficiency and severity of dengue infection in paediatric patients in Myanmar. A cross-sectional study...

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Autores principales: May, Win Lai, Kyaw, Myat Phone, Blacksell, Stuart D., Pukrittayakamee, Sasithon, Chotivanich, Kesinee, Hanboonkunupakarn, Borimas, Thein, Khin Nyo, Lim, Chae Seung, Thaipadungpanit, Janjira, Althaus, Thomas, Jittamala, Podjanee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6314580/
https://www.ncbi.nlm.nih.gov/pubmed/30601843
http://dx.doi.org/10.1371/journal.pone.0209204
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author May, Win Lai
Kyaw, Myat Phone
Blacksell, Stuart D.
Pukrittayakamee, Sasithon
Chotivanich, Kesinee
Hanboonkunupakarn, Borimas
Thein, Khin Nyo
Lim, Chae Seung
Thaipadungpanit, Janjira
Althaus, Thomas
Jittamala, Podjanee
author_facet May, Win Lai
Kyaw, Myat Phone
Blacksell, Stuart D.
Pukrittayakamee, Sasithon
Chotivanich, Kesinee
Hanboonkunupakarn, Borimas
Thein, Khin Nyo
Lim, Chae Seung
Thaipadungpanit, Janjira
Althaus, Thomas
Jittamala, Podjanee
author_sort May, Win Lai
collection PubMed
description Glucose-6-phosphate dehydrogenase (G6PD) deficiency may affect the clinical presentation of dengue due to the altered redox state in immune cells. We aimed to determine the association between G6PD deficiency and severity of dengue infection in paediatric patients in Myanmar. A cross-sectional study was conducted among paediatric patients aged 2–13 years with dengue in Yankin Children Hospital, Myanmar. One hundred and ninety-six patients positive for dengue infection, as determined via PCR or ELISA, were enrolled. Dengue severity was determined according to the 2009 WHO classification guidelines. Spectrophotometric assays determined G6PD levels. The adjusted median G6PD value of males in the study population was used to define various cut-off points according to the WHO classification guidelines. G6PD genotyping for Mahidol, Kaiping and Mediterranean mutations was performed for 128 out of 196 samples by real-time multiplex PCR. 51 of 196 (26.0%) patients had severe dengue. The prevalence of G6PD phenotype deficiency (< 60% activity) in paediatric patients was 14.8% (29/196), specifically, 13.6% (14/103) in males and 16.2% (15/93) in females. Severe deficiency (< 10% activity) accounted for 7.1% (14/196) of our cohort, occurring 11.7% (12/103) in males and 2.2% (2/93) in females. Among 128 samples genotyped, the G6PD gene mutations were detected in 19.5% (25/128) of patients, with 20.3% (13/ 64) in males and 18.8% (12/64) in females. The G6PD Mahidol mutation was 96.0% (24/25) while the G6PD Kaiping mutation was 4.0% (1/25). Severe dengue was not associated with G6PD enzyme deficiency or presence of the G6PD gene mutation. Thus, no association between G6PD deficiency and dengue severity could be detected. Trial registration: The study was registered following the WHO International Clinical Trials Registry Platform (WHO-ICTRP) on Thai Clinical Trials Registry (TCTR) website, registration number # TCTR20180720001
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spelling pubmed-63145802019-01-11 Impact of glucose-6-phosphate dehydrogenase deficiency on dengue infection in Myanmar children May, Win Lai Kyaw, Myat Phone Blacksell, Stuart D. Pukrittayakamee, Sasithon Chotivanich, Kesinee Hanboonkunupakarn, Borimas Thein, Khin Nyo Lim, Chae Seung Thaipadungpanit, Janjira Althaus, Thomas Jittamala, Podjanee PLoS One Research Article Glucose-6-phosphate dehydrogenase (G6PD) deficiency may affect the clinical presentation of dengue due to the altered redox state in immune cells. We aimed to determine the association between G6PD deficiency and severity of dengue infection in paediatric patients in Myanmar. A cross-sectional study was conducted among paediatric patients aged 2–13 years with dengue in Yankin Children Hospital, Myanmar. One hundred and ninety-six patients positive for dengue infection, as determined via PCR or ELISA, were enrolled. Dengue severity was determined according to the 2009 WHO classification guidelines. Spectrophotometric assays determined G6PD levels. The adjusted median G6PD value of males in the study population was used to define various cut-off points according to the WHO classification guidelines. G6PD genotyping for Mahidol, Kaiping and Mediterranean mutations was performed for 128 out of 196 samples by real-time multiplex PCR. 51 of 196 (26.0%) patients had severe dengue. The prevalence of G6PD phenotype deficiency (< 60% activity) in paediatric patients was 14.8% (29/196), specifically, 13.6% (14/103) in males and 16.2% (15/93) in females. Severe deficiency (< 10% activity) accounted for 7.1% (14/196) of our cohort, occurring 11.7% (12/103) in males and 2.2% (2/93) in females. Among 128 samples genotyped, the G6PD gene mutations were detected in 19.5% (25/128) of patients, with 20.3% (13/ 64) in males and 18.8% (12/64) in females. The G6PD Mahidol mutation was 96.0% (24/25) while the G6PD Kaiping mutation was 4.0% (1/25). Severe dengue was not associated with G6PD enzyme deficiency or presence of the G6PD gene mutation. Thus, no association between G6PD deficiency and dengue severity could be detected. Trial registration: The study was registered following the WHO International Clinical Trials Registry Platform (WHO-ICTRP) on Thai Clinical Trials Registry (TCTR) website, registration number # TCTR20180720001 Public Library of Science 2019-01-02 /pmc/articles/PMC6314580/ /pubmed/30601843 http://dx.doi.org/10.1371/journal.pone.0209204 Text en © 2019 May et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
May, Win Lai
Kyaw, Myat Phone
Blacksell, Stuart D.
Pukrittayakamee, Sasithon
Chotivanich, Kesinee
Hanboonkunupakarn, Borimas
Thein, Khin Nyo
Lim, Chae Seung
Thaipadungpanit, Janjira
Althaus, Thomas
Jittamala, Podjanee
Impact of glucose-6-phosphate dehydrogenase deficiency on dengue infection in Myanmar children
title Impact of glucose-6-phosphate dehydrogenase deficiency on dengue infection in Myanmar children
title_full Impact of glucose-6-phosphate dehydrogenase deficiency on dengue infection in Myanmar children
title_fullStr Impact of glucose-6-phosphate dehydrogenase deficiency on dengue infection in Myanmar children
title_full_unstemmed Impact of glucose-6-phosphate dehydrogenase deficiency on dengue infection in Myanmar children
title_short Impact of glucose-6-phosphate dehydrogenase deficiency on dengue infection in Myanmar children
title_sort impact of glucose-6-phosphate dehydrogenase deficiency on dengue infection in myanmar children
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6314580/
https://www.ncbi.nlm.nih.gov/pubmed/30601843
http://dx.doi.org/10.1371/journal.pone.0209204
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