Generating Vegfr3 reporter transgenic mouse expressing membrane-tagged Venus for visualization of VEGFR3 expression in vascular and lymphatic endothelial cells

Vascular endothelial growth factor receptor 3 (Vegfr3) has been widely used as a marker for lymphatic and vascular endothelial cells during mouse embryonic development and in adult mouse, making it valuable for studying angiogenesis and lymphangiogenesis under normal and pathological conditions. Her...

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Autores principales: Watanabe, Chisato, Matsushita, Jun, Azami, Takuya, Tsukiyama-Fujii, Setsuko, Tsukiyama, Tomoyuki, Mizuno, Seiya, Takahashi, Satoru, Ema, Masatsugu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6314617/
https://www.ncbi.nlm.nih.gov/pubmed/30601868
http://dx.doi.org/10.1371/journal.pone.0210060
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author Watanabe, Chisato
Matsushita, Jun
Azami, Takuya
Tsukiyama-Fujii, Setsuko
Tsukiyama, Tomoyuki
Mizuno, Seiya
Takahashi, Satoru
Ema, Masatsugu
author_facet Watanabe, Chisato
Matsushita, Jun
Azami, Takuya
Tsukiyama-Fujii, Setsuko
Tsukiyama, Tomoyuki
Mizuno, Seiya
Takahashi, Satoru
Ema, Masatsugu
author_sort Watanabe, Chisato
collection PubMed
description Vascular endothelial growth factor receptor 3 (Vegfr3) has been widely used as a marker for lymphatic and vascular endothelial cells during mouse embryonic development and in adult mouse, making it valuable for studying angiogenesis and lymphangiogenesis under normal and pathological conditions. Here, we report the generation of a novel transgenic (Tg) mouse that expresses a membrane-localized fluorescent reporter protein, Gap43-Venus, under the control of the Vegfr3 regulatory sequence. Vegfr3-Gap43-Venus BAC Tg recapitulated endogenous Vegfr3 expression in vascular and lymphatic endothelial cells during embryonic development and tumor development. Thus, this Tg mouse line contributes a valuable model to study angiogenesis and lymphangiogenesis in physiological and pathological contexts.
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spelling pubmed-63146172019-01-11 Generating Vegfr3 reporter transgenic mouse expressing membrane-tagged Venus for visualization of VEGFR3 expression in vascular and lymphatic endothelial cells Watanabe, Chisato Matsushita, Jun Azami, Takuya Tsukiyama-Fujii, Setsuko Tsukiyama, Tomoyuki Mizuno, Seiya Takahashi, Satoru Ema, Masatsugu PLoS One Research Article Vascular endothelial growth factor receptor 3 (Vegfr3) has been widely used as a marker for lymphatic and vascular endothelial cells during mouse embryonic development and in adult mouse, making it valuable for studying angiogenesis and lymphangiogenesis under normal and pathological conditions. Here, we report the generation of a novel transgenic (Tg) mouse that expresses a membrane-localized fluorescent reporter protein, Gap43-Venus, under the control of the Vegfr3 regulatory sequence. Vegfr3-Gap43-Venus BAC Tg recapitulated endogenous Vegfr3 expression in vascular and lymphatic endothelial cells during embryonic development and tumor development. Thus, this Tg mouse line contributes a valuable model to study angiogenesis and lymphangiogenesis in physiological and pathological contexts. Public Library of Science 2019-01-02 /pmc/articles/PMC6314617/ /pubmed/30601868 http://dx.doi.org/10.1371/journal.pone.0210060 Text en © 2019 Watanabe et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Watanabe, Chisato
Matsushita, Jun
Azami, Takuya
Tsukiyama-Fujii, Setsuko
Tsukiyama, Tomoyuki
Mizuno, Seiya
Takahashi, Satoru
Ema, Masatsugu
Generating Vegfr3 reporter transgenic mouse expressing membrane-tagged Venus for visualization of VEGFR3 expression in vascular and lymphatic endothelial cells
title Generating Vegfr3 reporter transgenic mouse expressing membrane-tagged Venus for visualization of VEGFR3 expression in vascular and lymphatic endothelial cells
title_full Generating Vegfr3 reporter transgenic mouse expressing membrane-tagged Venus for visualization of VEGFR3 expression in vascular and lymphatic endothelial cells
title_fullStr Generating Vegfr3 reporter transgenic mouse expressing membrane-tagged Venus for visualization of VEGFR3 expression in vascular and lymphatic endothelial cells
title_full_unstemmed Generating Vegfr3 reporter transgenic mouse expressing membrane-tagged Venus for visualization of VEGFR3 expression in vascular and lymphatic endothelial cells
title_short Generating Vegfr3 reporter transgenic mouse expressing membrane-tagged Venus for visualization of VEGFR3 expression in vascular and lymphatic endothelial cells
title_sort generating vegfr3 reporter transgenic mouse expressing membrane-tagged venus for visualization of vegfr3 expression in vascular and lymphatic endothelial cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6314617/
https://www.ncbi.nlm.nih.gov/pubmed/30601868
http://dx.doi.org/10.1371/journal.pone.0210060
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